gig gigas [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: gigprovided by FlyBase
Official Full Name: gigasprovided by FlyBase
Primary source: FLYBASE:FBgn0005198
Locus tag: Dmel_CG6975
See related: AllianceGenome:FB:FBgn0005198
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 6975; C1; CG6975; Dmel\CG6975; dTsc2; dTSC2; FBpp0074588; gig/Tsc2; Gigas; l(3)109; ME 109; tsc2; Tsc2; TsC2; TSC2; tsc2(gig); Tsc2/gig
Summary: Enables GTPase activator activity and protein kinase binding activity. Involved in several processes, including cellular response to chloroquine; negative regulation of developmental growth; and regulation of signal transduction. Located in cytosol. Part of TSC1-TSC2 complex. Is expressed in adult head and medullary zone of lymph gland primary lobe. Used to study tuberous sclerosis 2. Human ortholog(s) of this gene implicated in hepatic angiomyolipoma; lymphangioleiomyomatosis; medulloblastoma; tuberous sclerosis; and tuberous sclerosis 2. Orthologous to human TSC2 (TSC complex subunit 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 76F2-76F2; 3-46 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (20129181..20139010)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (20122281..20132110)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

our data identify the eIF4F complex as an important upstream regulator of TORC1, which acts via TSC2 to inactivate TORC1 upon withdrawal of amino acids
Title: eIF4A inactivates TORC1 in response to amino acid starvation.
Results identified Ser1338 in Drosophila TSC2 as the site for AMPK phosphorylation. Its mutation diminished the function of TSC2 in suppressing mTORC1-dependent cell growth in Drosophila wing epithelium.
Title: Identification of an AMPK phosphorylation site in Drosophila TSC2 (gigas) that regulate cell growth.
hyperactivation of TORC1 following the loss of TSC1/2 is detrimental to stem cell maintenance and multiple lineage differentiation in the Drosophila intestinal stem cell lineage, a mechanism that could be conserved in other stem cell lineages
Title: TSC1/2 regulates intestinal stem cell maintenance and lineage differentiation through Rheb-TORC1-S6K but independently of nutritional status or Notch regulation.
Data indicate that TORC1 activation by PVR involves Tsc1/Tsc2.
Title: Platelet-derived growth factor/vascular endothelial growth factor receptor inactivation by sunitinib results in Tsc1/Tsc2-dependent inhibition of TORC1.
deregulation of TORC1 induces activation of JNK, indicate that multiple cellular stresses are induced and contribute to the synthetic-lethal interactions between RB and TSC1/TSC2 inactivation.
Title: Deregulated G1-S control and energy stress contribute to the synthetic-lethal interactions between inactivation of RB and TSC1 or TSC2.
Tsc2 mutants showed a dramatic decrease in the levels of phosphorylated Akt, and interestingly, Akt mutants phenocopied Tsc2 mutants, leading to the hypothesis that Tsc2 and Akt might work via the same genetic pathway to regulate synapse growth.
Title: Tuberous sclerosis complex regulates Drosophila neuromuscular junction growth via the TORC2/Akt pathway.
study demonstrates that in the Drosophila lymph gland the tumor suppressors TSC and PTEN control blood progenitor proliferation through a common TOR- and 4EBP-dependent pathway
Title: Multifaceted roles of PTEN and TSC orchestrate growth and differentiation of Drosophila blood progenitors.
TSC1/2 and Myc coordinate the growth and division of Drosophila intestinal stem cells.
Title: Tuberous sclerosis complex and Myc coordinate the growth and division of Drosophila intestinal stem cells.
TSC1/2 prevents precocious GSC differentiation by inhibiting TORC1 activity and subsequently differentiation-promoting programs
Title: TSC1/2 tumour suppressor complex maintains Drosophila germline stem cells by preventing differentiation.
Inactivation of TSC2 and Rb synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species scavenger enzyme SOD2 induction
Title: Specific killing of Rb mutant cancer cells by inactivating TSC2.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to chloroquine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval midgut histolysis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lymph gland development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of TOR signaling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of TOR signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of TORC1 signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell size	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of insulin receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of organ growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of TORC2 signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of TORC2 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of small GTPase mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of terminal button organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to nutrient	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of TSC1-TSC2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TSC1-TSC2 complex	IDA Inferred from Direct Assay more info	PubMed
part_of TSC1-TSC2 complex	IEA Inferred from Electronic Annotation more info
part_of TSC1-TSC2 complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: gigas

Names: CG6975-PA; CG6975-PB; gig-PA; gig-PB; lethal(3)109; tuberous sclerosis complex 2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

20129181..20139010

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079453.3 → NP_524177.1 gigas, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524177.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9U9B0, Q9VW83

Related

FBpp0074588

Conserved Domains (4) summary

pfam02145
Location:1617 → 1802

Rap_GAP; Rap/ran-GAP

pfam03542
Location:566 → 882

Tuberin; Tuberin

pfam11864
Location:40 → 467

DUF3384; Domain of unknown function (DUF3384)

sd00044
Location:484 → 515

HEAT; HEAT repeat [structural motif]
NM_001275160.1 → NP_001262089.1 gigas, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262089.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PD17, Q9U9B0

Conserved Domains (4) summary

pfam02145
Location:1614 → 1799

Rap_GAP; Rap/ran-GAP

pfam03542
Location:566 → 882

Tuberin; Tuberin

pfam11864
Location:40 → 467

DUF3384; Domain of unknown function (DUF3384)

sd00044
Location:484 → 515

HEAT; HEAT repeat [structural motif]