NOS3 nitric oxide synthase 3 [Sus scrofa (pig)] - Gene

Summary

Official Symbol: NOS3provided by VGNC
Official Full Name: nitric oxide synthase 3provided by VGNC
Primary source: VGNC:VGNC:109472
See related: Ensembl:ENSSSCG00000016450
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Sus scrofa
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus
Also known as: NOS; cNOS; eNOS; EC-NOS; NOSIII
Expression: Ubiquitous expression in subcutaneous adipose (RPKM 14.9), lung (RPKM 9.6) and 8 other tissues See more
Orthologs: human mouse all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: chromosome: 18

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
106	current	Sscrofa11.1 (GCF_000003025.6)	18	NC_010460.4 (6209218..6228912, complement)
105	previous assembly	Sscrofa10.2 (GCF_000003025.5)	18	NC_010460.3 (6579253..6602572, complement)

Chromosome 18 - NC_010460.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Transcriptome data used for gene annotation Transcriptome data used for gene annotation
Description: Nine kinds of tissues and a mixture of multiple tissues were sequenced for genome annotation
BioProject: PRJNA311523
Publication: PMID 27646534
Analysis date: Thu Dec 14 17:10:49 2017

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eNOS protein and nitric oxide content in endothelial cells of conduit arteries is increased compared with the microcirculation endothelial cells.
Title: Endothelial nitric oxide synthase protein distribution and nitric oxide production in endothelial cells along the coronary vascular tree.
NOS3 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest.
Title: Comparison of endothelial nitric oxide synthase and endothelin-1 levels in kidneys removed from living pigs after cardiac arrest and brain death.
Rapid atrial pacing increases ADMA and down-regulates eNOS expression in an ADMA-independent manner.
Title: The impact of rapid atrial pacing on ADMA and endothelial NOS.
Icariin and icariside II may increase the eNOS expression through activating EGF-EGFR pathway in porcine aortic endothelial cells.
Title: [Effects of icariin and icariside II on eNOS expression and NOS activity in porcine aorta endothelial cells].
Data suggest that pig sperm contain bNOS, iNOS, and eNOS; up-regulation of NOS by leptin during acrosome reaction and inhibition of acrosome reaction by inhibitors of nitric oxide synthases suggests these enzymes are involved in acrosome reaction.
Title: Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm.
Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine.
Title: Periodic acceleration (pGz) acutely increases endothelial and neuronal nitric oxide synthase expression in endomyocardium of normal swine.
Data suggest that angiotensin II regulates nNOS and eNOS expression and NOS activity in afferent arterioles of the developing kidney via angiotensin 1 and 2 receptors.
Title: Angiotensin II regulates NOS expression in afferent arterioles of the developing porcine kidney.
Endothelial nitric oxide synthase mRNA expression was elevated in gestational day 50 intrauterine growth retardation placenta and areola compared to gd50 control.
Title: The effect of intrauterine growth retardation on the expression of developmental factors in porcine placenta subsequent to the initiation of placentation.
Oligonol prevented the impairment of eNOS activity induced by high glucose through reversing altered eNOS phosphorylation status.
Title: Beneficial effect of the oligomerized polyphenol oligonol on high glucose-induced changes in eNOS phosphorylation and dephosphorylation in endothelial cells.
Exercise training significantly increased total eNOS and phosphorylated levels of eNOS (pSer(1179)) in collateral-dependent arteries of experimental minipigs.
Title: Effects of exercise training on cellular mechanisms of endothelial nitric oxide synthase regulation in coronary arteries after chronic occlusion.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

MARC_6739-6740:982537599:1 (e-PCR)
- UniSTS:231255

NOS3 (e-PCR)
- UniSTS:503696

Gene Ontology Provided by RefSeq

Function	Evidence Code	Pubs
enables FMN binding	IEA Inferred from Electronic Annotation more info	PubMed
enables NADP binding	IEA Inferred from Electronic Annotation more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info	PubMed
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info	PubMed
enables nitric-oxide synthase activity	IEA Inferred from Electronic Annotation more info	PubMed

Process	Evidence Code	Pubs
involved_in nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: nitric oxide synthase 3

Names: NOS type III; constitutive NOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase, endothelial

NP_999460.1

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_214295.2 → NP_999460.1 nitric oxide synthase 3

See identical proteins and their annotated locations for NP_999460.1

Status: PROVISIONAL

Source sequence(s)

U59924

UniProtKB/Swiss-Prot

Q28969

UniProtKB/TrEMBL

Q06WL0

Related

ENSSSCP00000048632.2, ENSSSCT00000050205.3

Conserved Domains (4) summary

COG0369
Location:499 → 1161

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:70 → 482

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:524 → 700

Flavodoxin_1; Flavodoxin

cl06868
Location:764 → 1166

FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...