armi armitage [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: armiprovided by FlyBase
Official Full Name: armitageprovided by FlyBase
Primary source: FLYBASE:FBgn0041164
Locus tag: Dmel_CG11513
See related: AllianceGenome:FB:FBgn0041164
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: APC16; Armi; ARMI; CG11513; Dmel\CG11513
Summary: Enables 5'-3' RNA helicase activity; ATP hydrolysis activity; and piRNA binding activity. Involved in several processes, including dorsal appendage formation; negative regulation of gene expression; and oogenesis. Located in several cellular components, including Yb body; dendrite; and neuronal cell body. Is active in P granule; cytoplasmic side of mitochondrial outer membrane; and cytosol. Is extrinsic component of mitochondrial outer membrane. Is expressed in germarium; gonad; organism; and ovary. Used to study primary ovarian insufficiency. Human ortholog(s) of this gene implicated in spermatogenic failure 73. Orthologous to human MOV10L1 (Mov10 like RNA helicase 1). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: 63E1-63E1; 3-8 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (3461317..3466314)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (3461317..3466303)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Armitage determines Piwi-piRISC processing from precursor formation and quality control to inter-organelle translocation.
Title: Armitage determines Piwi-piRISC processing from precursor formation and quality control to inter-organelle translocation.
These findings shed light on distinct and collaborative requirements of Yb and Armi in transposon-targeting piRNA biogenesis.
Title: Distinct and Collaborative Functions of Yb and Armitage in Transposon-Targeting piRNA Biogenesis.
We propose that Armi shuttles between nuage and mitochondria, feeding precursor piRNAs generated by Ago3 cleavage into the Zucchini-dependent production of Aubergine- and Piwi-bound piRNAs on the mitochondrial surface.
Title: The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria.
A heterologous RNA that lacks complementary piRNAs is processed into piRNAs upon recruitment of several piRNA pathway factors. This processing requires Zuc and the helicase Armitage (Armi).
Title: Zucchini-dependent piRNA processing is triggered by recruitment to the cytoplasmic processing machinery.
We conclude that transcripts bound by Armi and Yb are identified as piRNA precursors, resulting in localization to cytoplasmic processing granules and their subsequent engagement by the resident piRNA biogenesis machinery.
Title: Recruitment of Armitage and Yb to a transcript triggers its phased processing into primary piRNAs in Drosophila ovaries.
roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis
Title: Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila.
Armi is an essential component of the somatic piRNA pathway.
Title: An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila.
Armi mutants support early steps in the RNAi pathway but are defective in the production of active RNA-induced silencing complex (RISC), which mediates target RNA destruction in RNAi. Our results suggest armi is required for RISC maturation
Title: RISC assembly defects in the Drosophila RNAi mutant armitage.
Mutations in Armitage and three components of the RNAi pathway disrupt oskar mRNA translational silencing, polarization of the microtubule cytoskeleton, and posterior localization of oskar mRNA (Armitage)
Title: The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 5'-3' RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-3' RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 5'-3' RNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	NAS Non-traceable Author Statement more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables piRNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal appendage formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived egg chamber formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule organizing center organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA damage checkpoint	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole plasm protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in secondary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in Yb body	IDA Inferred from Direct Assay more info	PubMed
located_in Yb body	IDA Inferred from Direct Assay more info	PubMed
located_in Yb body	IMP Inferred from Mutant Phenotype more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic side of mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: armitage

Names: CG11513-PB; CG11513-PC; CG11513-PD; armi-PB; armi-PC; armi-PD

NP_001014556.1

EC 3.6.4.13

NP_001246603.1

EC 3.6.4.13

NP_647816.2

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

3461317..3466314

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001014556.4 → NP_001014556.1 armitage, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001014556.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q59E56, Q6J5K9, Q9VZP4

UniProtKB/TrEMBL

A8WHG1

Conserved Domains (4) summary

COG1112
Location:840 → 1155

DNA2; Superfamily I DNA and/or RNA helicase [Replication, recombination and repair]

pfam13086
Location:698 → 902

AAA_11; AAA domain

pfam13245
Location:720 → 768

AAA_19; Part of AAA domain

cl22977
Location:909 → 1132

UvrD_C_2; UvrD-like helicase C-terminal domain
NM_139559.4 → NP_647816.2 armitage, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_647816.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q59E56, Q6J5K9, Q9VZP4

UniProtKB/TrEMBL

A8WHG1

Conserved Domains (4) summary

COG1112
Location:840 → 1155

DNA2; Superfamily I DNA and/or RNA helicase [Replication, recombination and repair]

pfam13086
Location:698 → 902

AAA_11; AAA domain

pfam13245
Location:720 → 768

AAA_19; Part of AAA domain

cl22977
Location:909 → 1132

UvrD_C_2; UvrD-like helicase C-terminal domain
NM_001259674.1 → NP_001246603.1 armitage, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246603.1

Status: REVIEWED

UniProtKB/TrEMBL

A8WHG1, M9NDG7

Conserved Domains (4) summary

COG1112
Location:806 → 1121

DNA2; Superfamily I DNA and/or RNA helicase [Replication, recombination and repair]

pfam13086
Location:664 → 868

AAA_11; AAA domain

pfam13245
Location:686 → 734

AAA_19; Part of AAA domain

cl22977
Location:875 → 1098

UvrD_C_2; UvrD-like helicase C-terminal domain