PolG2 DNA polymerase gamma subunit 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: PolG2provided by FlyBase
Official Full Name: DNA polymerase gamma subunit 2provided by FlyBase
Primary source: FLYBASE:FBgn0004407
Locus tag: Dmel_CG33650
See related: AllianceGenome:FB:FBgn0004407
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BG:DS00941.9; br16; CG33084; CG33650; CG8969; D-pol gamma-beta; Dmel\CG33650; DNA pol-gam.35; DNApol-gamma; DNApol-gamma.35; DNApol-gamma35; DNApolg-35; l(2)34De; l(2)br16; MtPolB; Pol gamma; pol gamma-alpha; pol gamma-beta; pol gamma35; Polg; PolG; POLG; polgamma; Polgamma; Polgamma-35; polgamma-beta; Polgamma-beta
Summary: Enables DNA/RNA hybrid binding activity and single-stranded RNA binding activity. Involved in mitochondrial DNA replication and mitochondrial genome maintenance. Located in mitochondrion. Part of gamma DNA polymerase complex. Used to study autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 4. Human ortholog(s) of this gene implicated in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 4 and mitochondrial DNA depletion syndrome. Orthologous to human POLG2 (DNA polymerase gamma 2, accessory subunit). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 34D1-34D1; 2-49 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (13831482..13833105, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (13831482..13833105, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Created an mtDNA mutator strain expressing a proofreading-deficient form of the mitochondrial DNA polymerase gamma. Mutator flies have a dramatically increased somatic mtDNA mutation frequency that correlates with the dosage of the proofreading-deficient polymerase. Mutator flies also exhibit mitochondrial dysfunction, shortened lifespan, a progressive locomotor deficit, and loss of dopaminergic neurons.
Title: Deleterious mitochondrial DNA point mutations are overrepresented in Drosophila expressing a proofreading-defective DNA polymerase γ.
The stimulatory effect of mtSSB on Pol gamma on these ssDNA templates is not species-specific.
Title: Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase γ by Organization of the Template DNA.
mtSSB uses distinct structural elements to interact functionally with its mtDNA replisome partners and to promote proper mtDNA replication in animal cells
Title: Reduced stimulation of recombinant DNA polymerase γ and mitochondrial DNA (mtDNA) helicase by variants of mitochondrial single-stranded DNA-binding protein (mtSSB) correlates with defects in mtDNA replication in animal cells.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA polymerase processivity factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA/RNA hybrid binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated DNA replication	IPI Inferred from Physical Interaction more info	PubMed
involved_in mitochondrial DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial genome maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of gamma DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed
part_of gamma DNA polymerase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: DNA polymerase gamma subunit 2

Names: CG33650-PA; DNA polymerase -gamma; DNA polymerase gamma; DNA polymerase gamma 35kD; DNA polymerase gamma 35kD subunit; DNA polymerase gamma subunit alpha; PolG2-PA; accessory subunit of mitochondrial DNA polymerase; accessory(beta)subunit; beta subunit; lethal 16 in the black-reduced region; lethal(2)34De; mitochondrial DNA polymerase accessory subunit; mitochondrial gamma polymerase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

13831482..13833105 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001032091.3 → NP_001027262.1 DNA polymerase gamma subunit 2 [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027262.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O02005, Q9NKE7, Q9VJV8

Conserved Domains (1) summary

cd02426
Location:228 → 361

Pol_gamma_b_Cterm; C-terminal domain of mitochondrial DNA polymerase gamma B subunit, which is required for processivity. Polymerase gamma replicates and repairs mitochondrial DNA. The c-terminal domain of its B subunit is strikingly similar to the anticodon-binding domain ...