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SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Bos taurus (domestic cattle) ]

Gene ID: 353352, updated on 10-Oct-2024

Summary

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Gene symbol
SPAM1
Gene description
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
See related
BGD:BT23575; Ensembl:ENSBTAG00000004640
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Bos taurus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos
Also known as
PH-20
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Genomic context

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See SPAM1 in Genome Data Viewer
Location:
chromosome: 4
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2023_09 current ARS-UCD2.0 (GCF_002263795.3) 4 NC_037331.1 (88202791..88222320)

Chromosome 4 - NC_037331.1Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA U3 Neighboring gene hyaluronidase 4 Neighboring gene uncharacterized LOC132345126 Neighboring gene small nucleolar RNA U3

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_037331.1 Chromosome 4 Reference ARS-UCD2.0 Primary Assembly

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. SPAM1 and PH-20 are two gene products expressed in bovine testis and present in sperm. Saindon AA, et al. Reproduction, 2018 Dec. PMID 30328344
  2. Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein. Maksimenko AV, et al. Biochemistry (Mosc), 2015 Mar. PMID 25761683
  3. Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates. Kakizaki I, et al. Biochem Biophys Res Commun, 2011 Mar 11. PMID 21320470
  4. SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization. Morin G, et al. Biol Reprod, 2010 Feb. PMID 19812301
  5. Development of a fluorescent substrate to measure hyaluronidase activity. Zhang LS, et al. Anal Biochem, 2008 Aug 1. PMID 18492482, Free PMC Article

See all (9) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PH-20 transcripts are found in bovine testis and the protein is present in ejaculated spermatozoa.
    Title: SPAM1 and PH-20 are two gene products expressed in bovine testis and present in sperm.
  2. Bovine testicular hyaluronidase 3D-model demonstrated lysine residue stratification during enzyme modification.
    Title: Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein.
  3. the effect of metal cations on the transglycosylation by bovine testicular hyaluronidase using as substrates metal salts of glycosaminoglycan chains was investigated.
    Title: Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.
  4. results clearly demonstrate that ejaculated bull sperm possess two forms of sperm adhesion molecule 1(SPAM1): one (epididymal) expressed at the surface, and one (testicular) that interacts with the zona pellucida after the acrosome exocytosis.
    Title: SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization.
  5. The hydrolysis Vm values strongly suggest that as the chain length decreases the hyaluronidase increasingly catalyses transglycosylation rather than hydrolysis.
    Title: Chain-length dependence of the kinetics of the hyaluronan hydrolysis catalyzed by bovine testicular hyaluronidase.
  6. The simplicity, sensitivity, and versatility of the fluorescent substrate (termed FRET-HA) suggest that it will have utility in a variety of assay platforms and should be a new tool for assessing hyaluronidase activity.
    Title: Development of a fluorescent substrate to measure hyaluronidase activity.
Submit: New GeneRIF Correction

General gene information

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Homology

Clone Names

  • MGC134590

Gene Ontology Provided by RefSeq

Function Evidence Code Pubs
enables hyalurononglucosaminidase activity IEA
Inferred from Electronic Annotation
more info
 PubMed 
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
 PubMed 
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
Inferred from Electronic Annotation
more info
 PubMed 
involved_in hyaluronan catabolic process IEA
Inferred from Electronic Annotation
more info
 PubMed 
Component Evidence Code Pubs
located_in acrosomal vesicle IEA
Inferred from Electronic Annotation
more info
 PubMed 

General protein information

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Preferred Names
sperm adhesion molecule 1
Names
hyaluronidase, zona pellucida binding
NP_001008413.3
XP_024846268.1
XP_024846269.1
XP_024846271.1
XP_059741399.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001008413.3 → NP_001008413.3  sperm adhesion molecule 1 precursor

    See identical proteins and their annotated locations for NP_001008413.3

    Status: VALIDATED

    Source sequence(s)
    NKLS02000004
    UniProtKB/TrEMBL
    F1MTV1, Q2YDK3
    Related
    ENSBTAP00000006089.3, ENSBTAT00000006089.4
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 371
    Glyco_hydro_56; Hyaluronidase

RefSeqs of Annotated Genomes: GCF_002263795.3-RS_2023_09

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference ARS-UCD2.0 Primary Assembly

Genomic

  1. NC_037331.1 Reference ARS-UCD2.0 Primary Assembly

    Range
    88202791..88222320
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024990500.2 → XP_024846268.1  sperm adhesion molecule 1 isoform X1

    UniProtKB/TrEMBL
    F1MTV1, Q2YDK3
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 371
    Glyco_hydro_56; Hyaluronidase

  2. XM_059885416.1 → XP_059741399.1  sperm adhesion molecule 1 isoform X1

    UniProtKB/TrEMBL
    F1MTV1

  3. XM_024990503.2 → XP_024846271.1  sperm adhesion molecule 1 isoform X2

    UniProtKB/TrEMBL
    Q2YDK3
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 341
    Glyco_hydro_56; Hyaluronidase

  4. XM_024990501.2 → XP_024846269.1  sperm adhesion molecule 1 isoform X1

    UniProtKB/TrEMBL
    F1MTV1, Q2YDK3
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 371
    Glyco_hydro_56; Hyaluronidase
Nucleotide Protein Strain
Heading Accession and Version

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