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ex expanded [ Drosophila melanogaster (fruit fly) ]

Gene ID: 33218, updated on 18-Sep-2024

Summary

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Official Symbol
exprovided by FlyBase
Official Full Name
expandedprovided by FlyBase
Primary source
FLYBASE:FBgn0004583
Locus tag
Dmel_CG4114
See related
AllianceGenome:FB:FBgn0004583
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
1270; brl; CG4114; Dmel\CG4114; Ex; EX; exp; Expanded; Frmd6; l(2)01270; l(2)ey
Summary
Enables DNA-binding transcription factor binding activity and kinase binding activity. Involved in several processes, including border follicle cell migration; negative regulation of imaginal disc growth; and positive regulation of hippo signaling. Located in apicolateral plasma membrane; cytoplasmic side of apical plasma membrane; and subapical part of cell. Part of Kibra-Ex-Mer complex. Colocalizes with cell-cell junction. Is expressed in embryonic/larval central nervous system; gland; imaginal disc; and neuroblast NB5-3. Used to study cancer. Orthologous to several human genes including FRMD1 (FERM domain containing 1). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
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Genomic context

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Location:
21C2-21C2; 2-0.7 cM
Exon count:
6
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (431227..448701)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (431227..448701)

Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Bardet-Biedl syndrome 8 Neighboring gene long non-coding RNA:CR43717 Neighboring gene croquemort Neighboring gene pseudo Neighboring gene shriveled

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Willing to Be Involved in Cancer. Gunn-Moore FJ, et al. Genes (Basel), 2016 Jul 18. PMID 27438856, Free PMC Article
  2. Expanded directly binds conserved regions of Fat to restrain growth via the Hippo pathway. Fulford AD, et al. J Cell Biol, 2023 May 1. PMID 37071483, Free PMC Article
  3. C-terminal-mediated homodimerization of Expanded is critical for its ability to promote Hippo signalling in Drosophila. Wang G, et al. FEBS Lett, 2022 Jul. PMID 35278215
  4. Toxicological effects of ciprofloxacin exposure to Drosophila melanogaster. Liu J, et al. Chemosphere, 2019 Dec. PMID 31549655
  5. AJUBA LIM Proteins Limit Hippo Activity in Proliferating Cells by Sequestering the Hippo Core Kinase Complex in the Cytosol. Jagannathan R, et al. Mol Cell Biol, 2016 Oct 15. PMID 27457617, Free PMC Article

See all (388) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Expanded directly binds conserved regions of Fat to restrain growth via the Hippo pathway.
    Title: Expanded directly binds conserved regions of Fat to restrain growth via the Hippo pathway.
  2. Merlin and expanded integrate cell signaling that regulates cyst stem cell proliferation in the Drosophila testis niche.
    Title: Merlin and expanded integrate cell signaling that regulates cyst stem cell proliferation in the Drosophila testis niche.
  3. Here, the authors show that the Casein Kinase 1 (CKI) family is required for Expanded phosphorylation. CKI expression promotes Expanded phosphorylation and interaction with Slimb/beta-TrCP.
    Title: Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.
  4. study suggests that a complex pattern of ex transcription results from integration of a uniform SWH signal with multiple other inputs, rather than from a pattern of SWH signaling.
    Title: Spatial regulation of expanded transcription in the Drosophila wing imaginal disc.
  5. Merlin and Kibra activate Hippo signaling in parallel to Expanded at a spatially distinct cellular domain, the medial apical cortex.
    Title: Kibra and Merlin Activate the Hippo Pathway Spatially Distinct from and Independent of Expanded.
  6. results show that Zyxin is a functional antagonist of Expanded in growth control
    Title: Zyxin antagonizes the FERM protein expanded to couple F-actin and Yorkie-dependent organ growth.
  7. The Ex-regulatory domain of Crb maps to the juxtamembrane FERM-binding motif (JM), a cytoskeletal interaction domain distinct from the PDZ-binding motif (PBM) through which Crb binds polarity factors.
    Title: Crumbs regulates Salvador/Warts/Hippo signaling in Drosophila via the FERM-domain protein Expanded.
  8. Kibra functions together with Mer and Ex in a protein complex localized to the apical domain of epithelial cells, and this protein complex regulates the Hippo kinase cascade via direct binding to Hpo and Sav.
    Title: Kibra functions as a tumor suppressor protein that regulates Hippo signaling in conjunction with Merlin and Expanded.
  9. Mer and Ex signal through the Hippo pathway in Drosophila.
    Title: The Hippo tumor-suppressor pathway regulates apical-domain size in parallel to tissue growth.
  10. new model whereby Expanded functions downstream of Warts, in concert with 14-3-3 proteins to sequester Yorkie in the cytoplasm, inhibiting growth activity of the Hippo pathway
    Title: The FERM-domain protein Expanded regulates Hippo pathway activity via direct interactions with the transcriptional activator Yorkie.
Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables DNA-binding transcription factor binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein sequestering activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in border follicle cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in compound eye morphogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in compound eye morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in compound eye photoreceptor cell differentiation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in compound eye photoreceptor cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytic recycling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in energy homeostasis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in follicle cell of egg chamber development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of TORC1 signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of cell population proliferation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of cell population proliferation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of imaginal disc growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of imaginal disc growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of organ growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of organ growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of apoptotic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of hippo signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of hippo signaling IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of hippo signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of hippo signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of hippo signaling IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of cell differentiation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of exit from mitosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of signal transduction IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of Kibra-Ex-Mer complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Kibra-Ex-Mer complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in apicolateral plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
colocalizes_with cell-cell junction IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasmic side of apical plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasmic side of apical plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in subapical part of cell IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
expanded
Names
CG4114-PA
CG4114-PB
braille
ex-PA
ex-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033779.5 Reference assembly

    Range
    431227..448701
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_057492.4 → NP_476840.2  expanded, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_476840.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q07436, Q9VPQ0
    UniProtKB/TrEMBL
    M9PC08
    Related
    FBpp0077719
    Conserved Domains (5) summary
    PHA03255
    Location:512 → 681
    PHA03255; BDLF3; Provisional
    smart00295
    Location:27 → 297
    B41; Band 4.1 homologues
    cd13185
    Location:298 → 405
    FERM_C_FRMD1_FRMD6; FERM domain C-lobe of FERM domain containing 1 and 6 proteins
    pfam00373
    Location:132 → 305
    FERM_M; FERM central domain
    pfam09379
    Location:40 → 98
    FERM_N; FERM N-terminal domain

  2. NM_001272894.1 → NP_001259823.1  expanded, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001259823.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q07436, Q9VPQ0
    UniProtKB/TrEMBL
    M9PC08
    Conserved Domains (5) summary
    PHA03255
    Location:512 → 681
    PHA03255; BDLF3; Provisional
    smart00295
    Location:27 → 297
    B41; Band 4.1 homologues
    cd13185
    Location:298 → 405
    FERM_C_FRMD1_FRMD6; FERM domain C-lobe of FERM domain containing 1 and 6 proteins
    pfam00373
    Location:132 → 305
    FERM_M; FERM central domain
    pfam09379
    Location:40 → 98
    FERM_N; FERM N-terminal domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
M9PC08 M9PC08
Q07436.3 Q07436

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