isl2a ISL LIM homeobox 2a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: isl2aprovided by ZNC
Official Full Name: ISL LIM homeobox 2aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-980526-562
See related: Ensembl:ENSDARG00000003971 AllianceGenome:ZFIN:ZDB-GENE-980526-562
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: isl2; ISL-2; zfIsl-2; wu:fc29g02
Summary: Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within blood vessel morphogenesis; exocrine pancreas development; and neuron differentiation. Predicted to be active in nucleus. Is expressed in several structures, including ectoderm; gut; liver and biliary system; nervous system; and neural tube. Orthologous to human ISL2 (ISL LIM homeobox 2). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 25

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	25	NC_007136.7 (32746014..32752001, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	25	NC_007136.6 (32338952..32345080, complement)

Chromosome 25 - NC_007136.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The isl2a transcription factor regulates pituitary development in zebrafish.
Title: The isl2a transcription factor regulates pituitary development in zebrafish.
Interaction of transcription factors Islet2 and Nr2f1b to control vascular patterning during zebrafish development.
Title: Interaction of transcription factors Islet2 and Nr2f1b to control vascular patterning during zebrafish development.
differentiation of the CaP motor neuron's stereotypic large inward and outward currents does not have a specific requirement for Islet2a.
Title: Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression.
Isl2a is expressed in the lateral posterior mesoderm prior to angioblast migration. Isl2a-deficient angioblasts show disorganized migration to the midline to form axial vessels. Its expression is negatively regulated by Notch activity. It positively regulates flt4, a VEGF-C receptor repressed by Notch during angiogenesis. Isl2a may be an intermediate between Notch signaling and genetic programs controlling angioblasts.
Title: The LIM-homeodomain transcription factor Islet2a promotes angioblast migration.
Prdm14 regulates caudal rimary axon outgrowth through activation of islet2 expression.
Title: Prdm14 acts upstream of islet2 transcription to regulate axon growth of primary motoneurons in zebrafish.
primary motoneuron subtypes are likely to be specified by factors that act in parallel to or upstream of islet1 and islet2
Title: Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
Results show that PlexinA4, a transmembrane protein known to be a co-receptor for class III semaphorins, acts downstream of Islet2 to promote branching of the peripheral axons of the primary sensory neurons.
Title: PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching.

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General gene information

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Markers

fc29g02.x1 (e-PCR)
- UniSTS:187919

isl3 (e-PCR), detects polymorphism
- UniSTS:186979

ISL2 (e-PCR)
- UniSTS:205452

D1S3693 (e-PCR)
- UniSTS:474271

isl2 (e-PCR), detects polymorphism
- UniSTS:238243

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angioblast cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within exocrine pancreas development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron fate specification	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within neuron fate specification	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuron fate specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within venous blood vessel morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: insulin gene enhancer protein isl-2a

Names: insulin gene enhancer protein isl-2; islet-2; islet-2A; islet2; islet2a

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_130970.1 → NP_571045.1 insulin gene enhancer protein isl-2a

See identical proteins and their annotated locations for NP_571045.1

Status: PROVISIONAL

Source sequence(s)

X88805

UniProtKB/Swiss-Prot

P53406

UniProtKB/TrEMBL

B3DH43

Related

ENSDARP00000020636.7, ENSDART00000012862.9

Conserved Domains (4) summary

cd09366
Location:27 → 81

LIM1_Isl; The first LIM domain of Isl, a member of LHX protein family

cd09374
Location:89 → 143

LIM2_Isl; The second LIM domain of Isl, a member of LHX protein family

smart00389
Location:191 → 247

HOX; Homeodomain

COG5576
Location:161 → 301

COG5576; Homeodomain-containing transcription factor [Transcription]

RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007136.7 Reference GRCz11 Primary Assembly

Range

32746014..32752001 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_021470443.2 → XP_021326118.1 insulin gene enhancer protein isl-2a isoform X2

UniProtKB/TrEMBL

A0A8M9PGP9

Conserved Domains (3) summary

cd09366
Location:27 → 81

LIM1_Isl; The first LIM domain of Isl, a member of LHX protein family

cd09374
Location:89 → 143

LIM2_Isl; The second LIM domain of Isl, a member of LHX protein family

smart00389
Location:192 → 248

HOX; Homeodomain
XM_005163157.5 → XP_005163214.1 insulin gene enhancer protein isl-2a isoform X1

UniProtKB/TrEMBL

A0A8M2BA75

Conserved Domains (3) summary

cd09366
Location:27 → 81

LIM1_Isl; The first LIM domain of Isl, a member of LHX protein family

cd09374
Location:89 → 143

LIM2_Isl; The second LIM domain of Isl, a member of LHX protein family

smart00389
Location:192 → 248

HOX; Homeodomain