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Med25 mediator complex subunit 25 [ Rattus norvegicus (Norway rat) ]

Gene ID: 292889, updated on 10-Oct-2024

Summary

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Official Symbol
Med25provided by RGD
Official Full Name
mediator complex subunit 25provided by RGD
Primary source
RGD:1305137
See related
EnsemblRapid:ENSRNOG00000020363 AllianceGenome:RGD:1305137
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
RGD1305137
Summary
Predicted to enable retinoid X receptor binding activity and transcription coactivator binding activity. Predicted to be involved in several processes, including positive regulation of chromatin binding activity; positive regulation of mediator complex assembly; and regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Predicted to be part of mediator complex and transcription regulator complex. Orthologous to human MED25 (mediator complex subunit 25). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Kidney (RPKM 63.2), Adrenal (RPKM 55.1) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Med25 in Genome Data Viewer
Location:
1q22
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 1 NC_086019.1 (104496447..104513061, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (95359961..95375827, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (100872240..100887864, complement)

Chromosome 1 - NC_086019.1Genomic Context describing neighboring genes Neighboring gene polynucleotide kinase 3'-phosphatase Neighboring gene PTOV1, extended AT-hook containing adaptor protein Neighboring gene fuzzy planar cell polarity protein Neighboring gene adaptor related protein complex 2 subunit alpha 1 Neighboring gene transfer RNA proline (anticodon AGG) 45 Neighboring gene testis-specific serine kinase substrate

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models. Leal A, et al. Neurogenetics, 2009 Oct. PMID 19290556, Free PMC Article
  2. Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25. Nakamura Y, et al. Nat Commun, 2011. PMID 21427722, Free PMC Article
  3. MED25 is distinct from TRAP220/MED1 in cooperating with CBP for retinoid receptor activation. Lee HK, et al. EMBO J, 2007 Aug 8. PMID 17641689, Free PMC Article
  4. The Mediator complex and transcription regulation. Poss ZC, et al. Crit Rev Biochem Mol Biol, 2013 Nov-Dec. PMID 24088064, Free PMC Article
  5. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, et al. Brief Bioinform, 2011 Sep. PMID 21873635, Free PMC Article

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
    Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables nuclear retinoic acid receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables nuclear retinoid X receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables transcription coactivator binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in biological_process ND
No biological Data available
more info
  
involved_in negative regulation of fibroblast proliferation ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of mediator complex assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of core mediator complex ISO
Inferred from Sequence Orthology
more info
  
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
part_of transcription regulator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription regulator complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 25
Names
mediator of RNA polymerase II transcription, subunit 25 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001170426.2 → NP_001163897.1  mediator of RNA polymerase II transcription subunit 25 isoform 3

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001
    UniProtKB/TrEMBL
    A6JAU0
    Related
    ENSRNOP00000094991.1, ENSRNOT00000117952.2
    Conserved Domains (4) summary
    pfam11232
    Location:401 → 546
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:228 → 384
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:664 → 746
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:13 → 225
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

  2. NM_001415793.1 → NP_001402722.1  mediator of RNA polymerase II transcription subunit 25 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001

  3. NM_001415794.1 → NP_001402723.1  mediator of RNA polymerase II transcription subunit 25 isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001
    Related
    ENSRNOP00000027675.7, ENSRNOT00000027675.8

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086019.1 Reference GRCr8

    Range
    104496447..104513061 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063284057.1 → XP_063140127.1  mediator of RNA polymerase II transcription subunit 25 isoform X2

  2. XM_006229010.5 → XP_006229072.3  mediator of RNA polymerase II transcription subunit 25 isoform X3

    UniProtKB/TrEMBL
    A0A8I6B1T5
    Related
    ENSRNOP00000112460.1, ENSRNOT00000159201.1
    Conserved Domains (6) summary
    pfam09770
    Location:612 → 743
    PAT1; Topoisomerase II-associated protein PAT1
    pfam11232
    Location:462 → 571
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:289 → 445
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:683 → 739
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:73 → 287
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
    pfam18658
    Location:754 → 812
    zf-C2H2_12; Zinc-finger C2H2-type

  3. XM_006229007.5 → XP_006229069.3  mediator of RNA polymerase II transcription subunit 25 isoform X1

    UniProtKB/TrEMBL
    A0A8I6AJG1, A0A8I6B1T5
    Related
    ENSRNOP00000093019.1, ENSRNOT00000105598.2
    Conserved Domains (6) summary
    pfam09770
    Location:648 → 779
    PAT1; Topoisomerase II-associated protein PAT1
    pfam11232
    Location:462 → 607
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:289 → 445
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:719 → 775
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:73 → 287
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
    pfam18658
    Location:790 → 848
    zf-C2H2_12; Zinc-finger C2H2-type

  4. XM_006229013.3 → XP_006229075.1  mediator of RNA polymerase II transcription subunit 25 isoform X9

    UniProtKB/TrEMBL
    D3ZJW8
    Conserved Domains (6) summary
    pfam09770
    Location:484 → 615
    PAT1; Topoisomerase II-associated protein PAT1
    pfam11232
    Location:298 → 443
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:125 → 281
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:555 → 611
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam18658
    Location:626 → 684
    zf-C2H2_12; Zinc-finger C2H2-type
    cl00057
    Location:1 → 123
    vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

  5. XM_063284073.1 → XP_063140143.1  mediator of RNA polymerase II transcription subunit 25 isoform X9

  6. XM_039104904.2 → XP_038960832.1  mediator of RNA polymerase II transcription subunit 25 isoform X9

    UniProtKB/TrEMBL
    D3ZJW8
    Conserved Domains (6) summary
    pfam09770
    Location:484 → 615
    PAT1; Topoisomerase II-associated protein PAT1
    pfam11232
    Location:298 → 443
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:125 → 281
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:555 → 611
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam18658
    Location:626 → 684
    zf-C2H2_12; Zinc-finger C2H2-type
    cl00057
    Location:1 → 123
    vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

  7. XM_063284064.1 → XP_063140134.1  mediator of RNA polymerase II transcription subunit 25 isoform X7

  8. XM_039104896.2 → XP_038960824.1  mediator of RNA polymerase II transcription subunit 25 isoform X6

    UniProtKB/TrEMBL
    A0A8I6B1T5
    Conserved Domains (4) summary
    pfam11232
    Location:461 → 606
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:289 → 444
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:694 → 782
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:73 → 287
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

  9. XM_006229011.5 → XP_006229073.3  mediator of RNA polymerase II transcription subunit 25 isoform X4

    UniProtKB/TrEMBL
    A0A8I6B1T5
    Conserved Domains (4) summary
    pfam11232
    Location:461 → 606
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:289 → 444
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:718 → 806
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:73 → 287
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

  10. XM_063284065.1 → XP_063140135.1  mediator of RNA polymerase II transcription subunit 25 isoform X8

  11. XM_006229012.5 → XP_006229074.3  mediator of RNA polymerase II transcription subunit 25 isoform X5

    UniProtKB/TrEMBL
    A0A8I6B1T5
    Conserved Domains (4) summary
    pfam11232
    Location:462 → 607
    Med25; Mediator complex subunit 25 PTOV activation and synapsin 2
    pfam11235
    Location:289 → 445
    Med25_SD1; Mediator complex subunit 25 synapsin 1
    pfam11244
    Location:695 → 783
    Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing
    pfam11265
    Location:73 → 287
    Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

  12. XM_063284077.1 → XP_063140147.1  mediator of RNA polymerase II transcription subunit 25 isoform X10

RNA

  1. XR_010064657.1 RNA Sequence

  2. XR_010064656.1 RNA Sequence

Nucleotide Protein Strain
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