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Eprs1 glutamyl-prolyl-tRNA synthetase 1 [ Rattus norvegicus (Norway rat) ]

Gene ID: 289352, updated on 10-Oct-2024

Summary

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Official Symbol
Eprs1provided by RGD
Official Full Name
glutamyl-prolyl-tRNA synthetase 1provided by RGD
Primary source
RGD:1310952
See related
EnsemblRapid:ENSRNOG00000002393 AllianceGenome:RGD:1310952
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Eprs
Summary
Predicted to enable several functions, including RNA stem-loop binding activity; proline-tRNA ligase activity; and protein homodimerization activity. Predicted to be involved in several processes, including cellular response to insulin stimulus; prolyl-tRNA aminoacylation; and regulation of long-chain fatty acid import into cell. Predicted to act upstream of or within cellular response to interferon-gamma. Predicted to be located in cytosol and plasma membrane. Predicted to be part of GAIT complex; aminoacyl-tRNA synthetase multienzyme complex; and ribonucleoprotein complex. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Liver (RPKM 829.2), Kidney (RPKM 361.3) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Eprs1 in Genome Data Viewer
Location:
13q26
Exon count:
35
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (99431955..99503510)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (96901548..96971966)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (103300911..103371651)

Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC134481540 Neighboring gene isoleucyl-tRNA synthetase 2, mitochondrial Neighboring gene microRNA 215 Neighboring gene 3'(2'), 5'-bisphosphate nucleotidase 1 Neighboring gene uncharacterized LOC100911719 Neighboring gene solute carrier family 30, member 10 Neighboring gene lysophospholipase-like 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Conformational changes in human prolyl-tRNA synthetase upon binding of the substrates proline and ATP and the inhibitor halofuginone. Son J, et al. Acta Crystallogr D Biol Crystallogr, 2013 Oct. PMID 24100331
  2. ATP-directed capture of bioactive herbal-based medicine on human tRNA synthetase. Zhou H, et al. Nature, 2013 Feb 7. PMID 23263184, Free PMC Article
  3. EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice. Arif A, et al. Nature, 2017 Feb 16. PMID 28178239, Free PMC Article
  4. Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation. Sampath P, et al. Cell, 2004 Oct 15. PMID 15479637
  5. Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages. Arif A, et al. Mol Cell Biol, 2012 Dec. PMID 23071094, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase binding ISO
Inferred from Sequence Orthology
more info
  
enables RNA stem-loop binding ISO
Inferred from Sequence Orthology
more info
  
enables glutamate-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables proline-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables proline-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in cellular response to insulin stimulus ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to type II interferon ISO
Inferred from Sequence Orthology
more info
  
involved_in glutamyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of translation ISO
Inferred from Sequence Orthology
more info
  
involved_in prolyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in prolyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of long-chain fatty acid import into cell ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of GAIT complex ISO
Inferred from Sequence Orthology
more info
  
part_of aminoacyl-tRNA synthetase multienzyme complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of aminoacyl-tRNA synthetase multienzyme complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
part_of ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
bifunctional glutamate/proline--tRNA ligase
Names
bifunctional aminoacyl-tRNA synthetase
proline--tRNA ligase-like
NP_001019409.1
XP_006250487.1
XP_006250488.1
XP_063128251.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001024238.1 → NP_001019409.1  bifunctional glutamate/proline--tRNA ligase isoform 1

    See identical proteins and their annotated locations for NP_001019409.1

    Status: PROVISIONAL

    Source sequence(s)
    AY383705
    UniProtKB/TrEMBL
    A0A8I5ZY25, Q6TXE9
    Related
    ENSRNOP00000033323.4, ENSRNOT00000038264.6
    Conserved Domains (9) summary
    cd10309
    Location:92 → 175
    GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
    PLN02907
    Location:30 → 685
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:993 → 1486
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00778
    Location:997 → 1260
    ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
    cd00862
    Location:1266 → 1486
    ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
    cd00936
    Location:800 → 849
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    pfam00458
    Location:727 → 779
    WHEP-TRS; WHEP-TRS domain
    pfam03950
    Location:478 → 655
    tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain
    cl00015
    Location:215 → 476
    nt_trans; nucleotidyl transferase superfamily

  2. NM_001429565.1 → NP_001416494.1  bifunctional glutamate/proline--tRNA ligase isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013
    UniProtKB/TrEMBL
    A0A8I6GJ92

  3. NM_001429566.1 → NP_001416495.1  bifunctional glutamate/proline--tRNA ligase isoform 3

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086031.1 Reference GRCr8

    Range
    99431955..99503510
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006250425.5 → XP_006250487.1  bifunctional glutamate/proline--tRNA ligase isoform X1

    Related
    ENSRNOP00000090936.1, ENSRNOT00000116204.2
    Conserved Domains (9) summary
    cd10309
    Location:74 → 157
    GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
    PLN02907
    Location:6 → 718
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:1026 → 1519
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00778
    Location:1030 → 1293
    ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
    cd00862
    Location:1299 → 1519
    ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
    cd00936
    Location:833 → 882
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    pfam00458
    Location:760 → 812
    WHEP-TRS; WHEP-TRS domain
    pfam00749
    Location:197 → 498
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
    pfam03950
    Location:511 → 688
    tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

  2. XM_006250426.5 → XP_006250488.1  bifunctional glutamate/proline--tRNA ligase isoform X2

    Related
    ENSRNOP00000071057.2, ENSRNOT00000085214.3
    Conserved Domains (9) summary
    cd10309
    Location:74 → 157
    GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
    PLN02907
    Location:6 → 711
    PLN02907; glutamate-tRNA ligase
    PRK08661
    Location:1019 → 1512
    PRK08661; prolyl-tRNA synthetase; Provisional
    cd00778
    Location:1023 → 1286
    ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
    cd00862
    Location:1292 → 1512
    ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
    cd00936
    Location:826 → 875
    WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
    pfam00458
    Location:753 → 805
    WHEP-TRS; WHEP-TRS domain
    pfam00749
    Location:197 → 502
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
    pfam03950
    Location:504 → 681
    tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

  3. XM_063272181.1 → XP_063128251.1  bifunctional glutamate/proline--tRNA ligase isoform X3

Nucleotide Protein Strain
Heading Accession and Version

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