Pou1f1 POU class 1 homeobox 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pou1f1provided by RGD
Official Full Name: POU class 1 homeobox 1provided by RGD
Primary source: RGD:3367
See related: EnsemblRapid:ENSRNOG00000000715 AllianceGenome:RGD:3367
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: GHF1; Pit1; GHF1A; PIT1Z
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and lncRNA binding activity. Involved in adenohypophysis development; negative regulation of transcription by RNA polymerase II; and positive regulation of transcription by RNA polymerase II. Located in chromatin. Human ortholog(s) of this gene implicated in panhypopituitarism and pituitary gland disease. Orthologous to human POU1F1 (POU class 1 homeobox 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Spleen (RPKM 2.5), Kidney (RPKM 2.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 11p12

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	11	NC_086029.1 (16763312..16781295)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	11	NC_051346.1 (3316818..3334804)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	11	NC_005110.4 (2645659..2662581)

Chromosome 11 - NC_086029.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Upregulation of Pit-1 protein is associated with Vascular Calcification.
Title: Calcium Overload Accelerates Phosphate-Induced Vascular Calcification Via Pit-1, but not the Calcium-Sensing Receptor.
Down-regulation of Pit-1 reduced prolactin levels during pulse periods.
Title: A Pit-1 Binding Site Adjacent to E-box133 in the Rat PRL Promoter is Necessary for Pulsatile Gene Expression Activity.
Blocking expression of endogenous PIT-1 induced a marked decrease of cell proliferation in somatolactotroph cells.
Title: Dose-dependent dual role of PIT-1 (POU1F1) in somatolactotroph cell proliferation and apoptosis.
Results suggest a sexually dimorphic response of lactotroph cells to the proliferative effects of EGF, with the PKCepsilon/ERK1/2 Pit-1 pathway being involved in this action.
Title: Epidermal growth factor induces a sexually dimorphic proliferative response of lactotroph cells through protein kinase C-ERK1/2-Pit-1 in vitro.
CEBPD and Pit1 interact and attenuate each other's binding to the PRL promoter.
Title: CEBPD suppresses prolactin expression and prolactinoma cell proliferation.
Expression of parathyroid Pit-1 in hyperphosphatemia-induced SHPT of CRF rats was upregulated. Pit-1 may mediate the stimulation to parathyroid gland by hyperphosphatemia.
Title: Overexpression of parathyroid pituitary-specific transcription factor (Pit)-1 in hyperphosphatemia-induced hyperparathyroidism of chronic renal failure rats.
Data suggest phosphorylation of Pit-1 (Pou1f1) at T220 serves as a mechanism to modulate Pit-1 target genes by regulating binding of Pit-1 to monomeric (Pit-1) versus dimeric (Ets-1/Pit-1) sites on DNA
Title: A Pit-1 threonine 220 phosphomimic reduces binding to monomeric DNA sites to inhibit Ras and estrogen stimulation of the prolactin gene promoter.
Activation of Syt1 gene expression is part of a mechanism mediating POU1F-induced differentiation of pituitary cells.
Title: Expression of the synaptotagmin I gene is enhanced by binding of the pituitary-specific transcription factor, POU1F1.
analysis of a Pit-1beta 26-amino acid dominant and independent repressor domain
Title: The 26-amino acid beta-motif of the Pit-1beta transcription factor is a dominant and independent repressor domain.
In the embryonic period, PROP1 coexists transiently with PIT1, which is the anterior pituitary-specific factor and is a target of PROP1, but not any hormones.
Title: PROP1 coexists with SOX2 and induces PIT1-commitment cells.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pit1 (e-PCR), detects polymorphism
- UniSTS:144183

D11Wox18 (e-PCR)
- UniSTS:518730

Pou1f1 (e-PCR), detects polymorphism
- UniSTS:547292

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISS Inferred from Sequence or Structural Similarity more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables lncRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in adenohypophysis development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell fate specification	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within determination of adult lifespan	ISO Inferred from Sequence Orthology more info
acts_upstream_of inositol trisphosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pituitary gland development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of inositol trisphosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of multicellular organism growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of insulin-like growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within somatotropin secreting cell development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within somatotropin secreting cell differentiation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromatin	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: pituitary-specific positive transcription factor 1

Names: GHF-1; POU domain, class 1, transcription factor 1; Pituary specific transcription factor 1 (growth hormone factor 1); growth hormone factor 1; homeodomain protein for Growth Hormone (GH); pit-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013008.3 → NP_037140.2 pituitary-specific positive transcription factor 1

See identical proteins and their annotated locations for NP_037140.2

Status: PROVISIONAL

Source sequence(s)

BC061563

UniProtKB/TrEMBL

Q6P7Q7, Q6P7Q8

Conserved Domains (2) summary

smart00352
Location:124 → 198

POU; Found in Pit-Oct-Unc transcription factors

pfam00046
Location:217 → 270

Homeobox; Homeobox domain