Klrk1 killer cell lectin like receptor K1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Klrk1provided by RGD
Official Full Name: killer cell lectin like receptor K1provided by RGD
Primary source: RGD:3180
See related: EnsemblRapid:ENSRNOG00000061739 AllianceGenome:RGD:3180
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: NKG2D; Nkrp2
Summary: Predicted to enable several functions, including MHC class I protein binding activity; MHC class Ib receptor activity; and identical protein binding activity. Involved in positive regulation of apoptotic process; positive regulation of myeloid dendritic cell activation; and positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target. Located in cell surface. Used to study histiocytoma. Human ortholog(s) of this gene implicated in tropical spastic paraparesis. Orthologous to several human genes including KLRC4-KLRK1 (KLRC4-KLRK1 readthrough). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 177.8), Thymus (RPKM 69.5) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q42

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (164767377..164778453, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (163079887..163092434, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (163392652..163403735, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Blockade of NKG2D significantly attenuated ischemia-reperfusion injury in a cardiac transplantation model.
Title: NKG2D blockade significantly attenuates ischemia-reperfusion injury in a cardiac transplantation model.
Natural killer (NK) cell-activating receptor NKG2D is important for NK-mediated killing of hepatoma McA tumor cells; NK cell cytotoxicity against McA-RH7777 is mediated by both NKp30 and NKG2D.
Title: Distinct roles for the NK cell-activating receptors in mediating interactions with dendritic cells and tumor cells.
the splice forms of NKG2D arise from alternative promoters and that the NKG2D-L promoter is derived from a B1 retrotransposon.
Title: Creation of the two isoforms of rodent NKG2D was driven by a B1 retrotransposon insertion.
In vitro cytotoxic assays reveal that a subset of rat monocytes/macrophages expressing both CD4 and CD8 antigens can kill certain tumor cells in a cell-cell contact-dependent manner through recognition of NKG2D ligands.
Title: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism.
A novel role of Irp94 as a ligand for NKR-P2 on DCs, which in turn executes both innate and adaptive immunity, was demonstrated.
Title: The ischemia-responsive protein 94 (Irp94) activates dendritic cells through NK cell receptor protein-2/NK group 2 member D (NKR-P2/NKG2D) leading to their maturation.
We have reported the involvement of NKR-P2 in rat dendritic cell (DC) activation. We demonstrate the potential of agonistic anti-NKR-P2 mAb (1A6), which mimics the NKR-P2 ligand and induces activation and maturation of DCs.
Title: Cross-linking a mAb to NKR-P2/NKG2D on dendritic cells induces their activation and maturation leading to enhanced anti-tumor immune response.
NKG2D acts as a natural cytotoxicity receptor to stimulate perforin-mediated elimination of ligand-expressing tumor cells.
Title: Cutting edge: tumor rejection mediated by NKG2D receptor-ligand interaction is dependent upon perforin.
NKG2D is required for cytolysis of tumor cells, including autologous tumor cells from patients with ovarian cancer, and in vivo antitumor activity
Title: Silencing human NKG2D, DAP10, and DAP12 reduces cytotoxicity of activated CD8+ T cells and NK cells.

Submit: New GeneRIF Correction

General gene information

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Markers

RH142207 (e-PCR)
- UniSTS:231457

D6H12S2489E (e-PCR), detects polymorphism
- UniSTS:142578

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables MHC class I protein binding	IEA Inferred from Electronic Annotation more info
enables MHC class I protein binding	ISO Inferred from Sequence Orthology more info
enables MHC class Ib receptor activity	IEA Inferred from Electronic Annotation more info
enables MHC class Ib receptor activity	ISO Inferred from Sequence Orthology more info
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adaptive immune response	IEA Inferred from Electronic Annotation more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to lipopolysaccharide	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in defense response to Gram-positive bacterium	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within defense response to Gram-positive bacterium	ISO Inferred from Sequence Orthology more info
involved_in natural killer cell activation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within natural killer cell activation	ISO Inferred from Sequence Orthology more info
involved_in natural killer cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of natural killer cell chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of myeloid dendritic cell activation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of natural killer cell mediated cytotoxicity	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of natural killer cell mediated cytotoxicity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of natural killer cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of natural killer cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of natural killer cell mediated cytotoxicity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of type II interferon production	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of type II interferon production	ISO Inferred from Sequence Orthology more info
involved_in stimulatory C-type lectin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within stimulatory C-type lectin receptor signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISS Inferred from Sequence or Structural Similarity more info
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NKG2-D type II integral membrane protein

Names: NK cell receptor D; NK lectin-like receptor; NKG2-D-activating NK receptor; NKLLR; NKR-P2, ortholog of human NKG2D; killer cell lectin-like receptor subfamily K, member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_133512.2 → NP_598196.1 NKG2-D type II integral membrane protein

See identical proteins and their annotated locations for NP_598196.1

Status: VALIDATED

Source sequence(s)

AF009511, FQ241294

UniProtKB/Swiss-Prot

O70215

UniProtKB/TrEMBL

A0A8L2URA6, A6IM72

Related

ENSRNOP00000071035.2, ENSRNOT00000091890.3

Conserved Domains (2) summary

cd03593
Location:98 → 212

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)

PHA03097
Location:45 → 213

PHA03097; C-type lectin-like protein; Provisional

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

164767377..164778453 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039107081.2 → XP_038963009.1 NKG2-D type II integral membrane protein isoform X1

UniProtKB/TrEMBL

A0A0G2JZG3, A0A8L2URA6, A6IM69

Conserved Domains (1) summary

cd03593
Location:112 → 226

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)
XM_006237077.5 → XP_006237139.1 NKG2-D type II integral membrane protein isoform X3

See identical proteins and their annotated locations for XP_006237139.1

UniProtKB/Swiss-Prot

O70215

UniProtKB/TrEMBL

A0A8L2URA6, A6IM72

Conserved Domains (2) summary

cd03593
Location:98 → 212

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)

PHA03097
Location:45 → 213

PHA03097; C-type lectin-like protein; Provisional
XM_006237075.5 → XP_006237137.1 NKG2-D type II integral membrane protein isoform X2

See identical proteins and their annotated locations for XP_006237137.1

UniProtKB/TrEMBL

A0A8L2URA6, A6IM70

Conserved Domains (2) summary

cd03593
Location:110 → 224

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)

PHA03097
Location:57 → 225

PHA03097; C-type lectin-like protein; Provisional
XM_006237073.4 → XP_006237135.1 NKG2-D type II integral membrane protein isoform X1

See identical proteins and their annotated locations for XP_006237135.1

UniProtKB/TrEMBL

A0A0G2JZG3, A0A8L2URA6, A6IM69

Related

ENSRNOP00000110632.1, ENSRNOT00000146938.1

Conserved Domains (1) summary

cd03593
Location:112 → 226

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)
XM_006237076.5 → XP_006237138.1 NKG2-D type II integral membrane protein isoform X2

See identical proteins and their annotated locations for XP_006237138.1

UniProtKB/TrEMBL

A0A8L2URA6, A6IM70

Conserved Domains (2) summary

cd03593
Location:110 → 224

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)

PHA03097
Location:57 → 225

PHA03097; C-type lectin-like protein; Provisional
XM_006237074.5 → XP_006237136.1 NKG2-D type II integral membrane protein isoform X2

See identical proteins and their annotated locations for XP_006237136.1

UniProtKB/TrEMBL

A0A8L2URA6, A6IM70

Conserved Domains (2) summary

cd03593
Location:110 → 224

CLECT_NK_receptors_like; C-type lectin-like domain (CTLD) of the type found in natural killer cell receptors (NKRs)

PHA03097
Location:57 → 225

PHA03097; C-type lectin-like protein; Provisional