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Micall2 MICAL-like 2 [ Mus musculus (house mouse) ]

Gene ID: 231830, updated on 18-Sep-2024

Summary

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Official Symbol
Micall2provided by MGI
Official Full Name
MICAL-like 2provided by MGI
Primary source
MGI:MGI:2444818
See related
Ensembl:ENSMUSG00000036718 AllianceGenome:MGI:2444818
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
JRAB; MICAL-L2; mFLJ00139; A930021H16Rik
Summary
Enables cytoskeletal protein binding activity and small GTPase binding activity. Involved in several processes, including actin cytoskeleton organization; bicellular tight junction assembly; and substrate adhesion-dependent cell spreading. Acts upstream of or within endocytic recycling. Located in several cellular components, including actin filament bundle; bicellular tight junction; and recycling endosome. Colocalizes with stress fiber. Is expressed in several structures, including chondrocranium; liver lobe; medulla oblongata basal plate; submandibular gland primordium; and vibrissa. Orthologous to human MICALL2 (MICAL like 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in colon adult (RPKM 6.8), stomach adult (RPKM 6.2) and 27 other tissues See more
Orthologs
human all
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Genomic context

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See Micall2 in Genome Data Viewer
Location:
5 G2; 5 78.79 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (139692447..139722088, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (139706692..139736333, complement)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene UNC homeobox Neighboring gene predicted gene, 46926 Neighboring gene STARR-seq mESC enhancer starr_14696 Neighboring gene STARR-seq mESC enhancer starr_14697 Neighboring gene STARR-positive B cell enhancer ABC_E8087 Neighboring gene integrator complex subunit 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:140252978-140253165 Neighboring gene microRNA 7037 Neighboring gene STARR-positive B cell enhancer ABC_E4803 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:140268130-140268313 Neighboring gene v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains. Miyake K, et al. Sci Rep, 2019 Sep 5. PMID 31488862, Free PMC Article
  2. Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development. Sakane A, et al. J Biol Chem, 2012 Dec 14. PMID 23100251, Free PMC Article
  3. Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2. Sakane A, et al. Mol Cell Biol, 2010 Feb. PMID 20008558, Free PMC Article
  4. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Nakatsuji H, et al. Mol Cell Biol, 2008 May. PMID 18332111, Free PMC Article
  5. JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. Terai T, et al. Mol Biol Cell, 2006 May. PMID 16525024, Free PMC Article

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. the LIM domain, independent of the C-terminus, is necessary for the function of open-form JRAB/MICAL-L2
    Title: Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains.
  2. Interaction between JRAB and filamin regulates JRABDeltaCC-induced cell spreading.
    Title: Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.
  3. Rab13 and JRAB regulate reorganization of the actin cytoskeleton throughout epithelial junctional development.
    Title: Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development.
  4. show that Rab13 regulates neurite outgrowth in the rat pheochromocytoma cell line PC12 through an interaction with JRAB/MICAL-L2.
    Title: Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.
  5. These results suggest that the interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
    Title: The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
  6. Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation.
    Title: Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.
  7. JRAB/MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton.
    Title: JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ00139

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables actin filament binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables actinin binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables filamin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables filamin binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables small GTPase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament polymerization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament polymerization ISO
Inferred from Sequence Orthology
more info
  
involved_in bicellular tight junction assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within endocytic recycling IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in endocytic recycling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuron projection development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuron projection development ISO
Inferred from Sequence Orthology
more info
  
involved_in substrate adhesion-dependent cell spreading IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in actin filament bundle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in bicellular tight junction IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell-cell junction IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in neuron projection ISO
Inferred from Sequence Orthology
more info
  
located_in neuron projection ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in recycling endosome IDA
Inferred from Direct Assay
more info
 PubMed 
colocalizes_with stress fiber IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
MICAL-like protein 2
Names
junctional Rab13-binding protein
molecule interacting with CasL-like 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_174850.3 → NP_777275.2  MICAL-like protein 2

    See identical proteins and their annotated locations for NP_777275.2

    Status: VALIDATED

    Source sequence(s)
    AB182579, BY346995, BY700282
    Consensus CDS
    CCDS39353.2
    UniProtKB/Swiss-Prot
    Q3TN34, Q3TPY3, Q3UB65
    UniProtKB/TrEMBL
    Q5DU62
    Related
    ENSMUSP00000039707.11, ENSMUST00000044642.14
    Conserved Domains (3) summary
    cd09444
    Location:188 → 241
    LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins
    pfam00307
    Location:5 → 102
    CH; Calponin homology (CH) domain
    pfam12130
    Location:842 → 978
    DUF3585; Protein of unknown function (DUF3585)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    139692447..139722088 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036165097.1 → XP_036020990.1  MICAL-like protein 2 isoform X1

    UniProtKB/TrEMBL
    E9PZD2, Q80UK4
    Related
    ENSMUSP00000127937.2, ENSMUST00000170773.6
    Conserved Domains (4) summary
    cd09444
    Location:105 → 158
    LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins
    pfam05109
    Location:361 → 695
    Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
    pfam12130
    Location:764 → 890
    DUF3585; Protein of unknown function (DUF3585)
    cl00030
    Location:1 → 24
    CH_SF; calponin homology (CH) domain superfamily

  2. XM_011240974.4 → XP_011239276.1  MICAL-like protein 2 isoform X1

    UniProtKB/TrEMBL
    E9PZD2, Q80UK4
    Conserved Domains (4) summary
    cd09444
    Location:105 → 158
    LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins
    pfam05109
    Location:361 → 695
    Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
    pfam12130
    Location:764 → 890
    DUF3585; Protein of unknown function (DUF3585)
    cl00030
    Location:1 → 24
    CH_SF; calponin homology (CH) domain superfamily
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q3TN34.1 GenPept UniProtKB/Swiss-Prot:Q3TN34

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