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CILK1 ciliogenesis associated kinase 1 [ Homo sapiens (human) ]

Gene ID: 22858, updated on 5-Mar-2024

Summary

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Official Symbol
CILK1provided by HGNC
Official Full Name
ciliogenesis associated kinase 1provided by HGNC
Primary source
HGNC:HGNC:21219
See related
Ensembl:ENSG00000112144 MIM:612325; AllianceGenome:HGNC:21219
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ECO; ICK; MRK; LCK2; hICK; EJM10
Summary
Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in adrenal (RPKM 9.9), placenta (RPKM 5.9) and 25 other tissues See more
Orthologs
mouse all
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Genomic context

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Location:
6p12.1
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (53001303..53061824, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (52840881..52901428, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (52866101..52926622, complement)

Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_92160 Neighboring gene glutathione S-transferase alpha 4 Neighboring gene ReSE screen-validated silencer GRCh37_chr6:52860003-52860234 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24693 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17287 Neighboring gene RNA component of 7SK nuclear ribonucleoprotein Neighboring gene uncharacterized LOC128092246 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:52908870-52909424 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17288 Neighboring gene uncharacterized LOC128031835 Neighboring gene F-box protein 9 Neighboring gene RNA, 7SL, cytoplasmic 244, pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Anterograde trafficking of ciliary MAP kinase-like ICK/CILK1 by the intraflagellar transport machinery is required for intraciliary retrograde protein trafficking. Nakamura K, et al. J Biol Chem, 2020 Sep 18. PMID 32732286, Free PMC Article
  2. Functional Alterations in Ciliogenesis-Associated Kinase 1 (CILK1) that Result from Mutations Linked to Juvenile Myoclonic Epilepsy. Wang EJ, et al. Cells, 2020 Mar 12. PMID 32178256, Free PMC Article
  3. Ciliogenesis associated kinase 1: targets and functions in various organ systems. Fu Z, et al. FEBS Lett, 2019 Nov. PMID 31506943, Free PMC Article
  4. Intestinal cell kinase, a MAP kinase-related kinase, regulates proliferation and G1 cell cycle progression of intestinal epithelial cells. Fu Z, et al. Am J Physiol Gastrointest Liver Physiol, 2009 Oct. PMID 19696144, Free PMC Article
  5. Activation of a nuclear Cdc2-related kinase within a mitogen-activated protein kinase-like TDY motif by autophosphorylation and cyclin-dependent protein kinase-activating kinase. Fu Z, et al. Mol Cell Biol, 2005 Jul. PMID 15988018, Free PMC Article

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Scaffold Protein KATNIP Enhances CILK1 Control of Primary Cilia.
    Title: The Scaffold Protein KATNIP Enhances CILK1 Control of Primary Cilia.
  2. BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1.
    Title: BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1.
  3. Functional Alterations in Ciliogenesis-Associated Kinase 1 (CILK1) that Result from Mutations Linked to Juvenile Myoclonic Epilepsy.
    Title: Functional Alterations in Ciliogenesis-Associated Kinase 1 (CILK1) that Result from Mutations Linked to Juvenile Myoclonic Epilepsy.
  4. Anterograde trafficking of ciliary MAP kinase-like ICK/CILK1 by the intraflagellar transport machinery is required for intraciliary retrograde protein trafficking.
    Title: Anterograde trafficking of ciliary MAP kinase-like ICK/CILK1 by the intraflagellar transport machinery is required for intraciliary retrograde protein trafficking.
  5. Previously known as intestinal cell kinase because it was cloned from that origin, CILK1 is now recognized as a widely expressed and highly conserved. Mutations in the human CILK1 gene have been associated with ciliopathies. Thus, CILK1 has a fundamental role in the function of the cilium. Several candidate substrates have been proposed for CILK1. [review]
    Title: Ciliogenesis associated kinase 1: targets and functions in various organ systems.
  6. Study demonstrates that human ICK (intestinal cell kinase) that authors herein call CAPK (ciliopathy-associated protein kinase) interacts with human KIF3A and phosphorylates a conserved site Thr672 both in vitro and in vivo. Long, unstructured, non-catalytic carboxyl-terminal domain (CTD) of CAPK is required for this interaction with and phosphorylation of KIF3A.
    Title: Ciliopathy-Associated Protein Kinase ICK Requires Its Non-Catalytic Carboxyl-Terminal Domain for Regulation of Ciliogenesis.
  7. Our data provide evidence that heterozygous variants in ICK caused juvenile myoclonic epilepsy in 7% of the patients included in our analysis. Variant ICK affects cell processes that help explain microdysgenesis and polyspike networks observed on EEG in juvenile myoclonic epilepsy.
    Title: Variant Intestinal-Cell Kinase in Juvenile Myoclonic Epilepsy.
  8. These data identify ICK as an short rib polydactyly syndromes (SRPS)-associated gene and reveal that abnormalities in signalling pathways contribute to defective skeletogenesis
    Title: An inactivating mutation in intestinal cell kinase, ICK, impairs hedgehog signalling and causes short rib-polydactyly syndrome.
  9. findings suggest that increased ICK expression/activity in response to protein deprivation likely provides a novel protective mechanism to limit apoptosis and support compensatory mucosal growth under nutritional stress.
    Title: Intestinal cell kinase is a novel participant in intestinal cell signaling responses to protein malnutrition.
  10. the expressions of ICK/MAK/MOK proteins in the intestinal tract can be differentially and dynamically regulated, implicating a significant functional diversity within this group of protein kinases.
    Title: Distinct expression patterns of ICK/MAK/MOK protein kinases in the intestine implicate functional diversity.
Submit: New GeneRIF Correction See all GeneRIFs (20)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description Tests
Endocrine-cerebro-osteodysplasia syndrome
MedGen: C2675227 OMIM: 612651 GeneReviews: Not available
Compare labs
Epilepsy, juvenile myoclonic, susceptibility to, 10
MedGen: C4693613 OMIM: 617924 GeneReviews: Not available
Compare labs

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • KIAA0936, MGC46090

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cilium assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cilium assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in intraciliary anterograde transport ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in intraciliary retrograde transport ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in intraciliary transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intraciliary transport ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in signal transduction TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in ciliary basal body ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in ciliary base ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in ciliary tip IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in cilium IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cilium ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in fibrillar center IDA
Inferred from Direct Assay
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase ICK
Names
MAK-related kinase
iciliogenesis associated kinase 1
intestinal cell (MAK-like) kinase
intestinal cell kinase
laryngeal cancer kinase 2
serine/threonine protein kinase
NP_001362326.1
NP_001362327.1
NP_001362328.1
NP_001362329.1
NP_001362330.1
NP_001362331.1
NP_055735.1
NP_057597.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_012159.2 RefSeqGene

    Range
    4979..65500
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001375397.1 → NP_001362326.1  serine/threonine-protein kinase ICK isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS93933.1
    UniProtKB/TrEMBL
    A0A7I2PIU1, B3KQG4
    Related
    ENSP00000263043.8, ENST00000350082.10
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  2. NM_001375398.1 → NP_001362327.1  serine/threonine-protein kinase ICK isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  3. NM_001375399.1 → NP_001362328.1  serine/threonine-protein kinase ICK isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  4. NM_001375400.1 → NP_001362329.1  serine/threonine-protein kinase ICK isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  5. NM_001375401.1 → NP_001362330.1  serine/threonine-protein kinase ICK isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  6. NM_001375402.1 → NP_001362331.1  serine/threonine-protein kinase ICK isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  7. NM_014920.5 → NP_055735.1  serine/threonine-protein kinase ICK isoform 1

    See identical proteins and their annotated locations for NP_055735.1

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Related
    ENSP00000501692.1, ENST00000676107.1
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

  8. NM_016513.5 → NP_057597.2  serine/threonine-protein kinase ICK isoform 1

    See identical proteins and their annotated locations for NP_057597.2

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581
    Consensus CDS
    CCDS4949.1
    UniProtKB/Swiss-Prot
    A7MD41, O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, Q9UPZ9
    UniProtKB/TrEMBL
    B3KQG4
    Related
    ENSP00000349458.3, ENST00000356971.3
    Conserved Domains (1) summary
    cd07830
    Location:4 → 284
    STKc_MAK_like; Catalytic domain of Male germ cell-Associated Kinase-like Serine/Threonine Kinases

RNA

  1. NR_164684.1 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL031178, AL162581

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

    Range
    53001303..53061824 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060930.1 Alternate T2T-CHM13v2.0

    Range
    52840881..52901428 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_173041.1: Suppressed sequence

    Description
    NM_173041.1: This RefSeq record was removed by NCBI staff. Contact info@ncbi.nlm.nih.gov for further information.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9UPZ9.1 GenPept UniProtKB/Swiss-Prot:Q9UPZ9

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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