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Dars2 aspartyl-tRNA synthetase 2 (mitochondrial) [ Mus musculus (house mouse) ]

Gene ID: 226539, updated on 2-Nov-2024

Summary

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Official Symbol
Dars2provided by MGI
Official Full Name
aspartyl-tRNA synthetase 2 (mitochondrial)provided by MGI
Primary source
MGI:MGI:2442510
See related
Ensembl:ENSMUSG00000026709 AllianceGenome:MGI:2442510
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
aspRS; 5830468K18Rik
Summary
Predicted to enable aspartate-tRNA ligase activity; aspartate-tRNA(Asn) ligase activity; and protein homodimerization activity. Predicted to be involved in aspartyl-tRNA aminoacylation and mitochondrial asparaginyl-tRNA aminoacylation. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; midbrain; and telencephalon. Orthologous to human DARS2 (aspartyl-tRNA synthetase 2, mitochondrial). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in liver E14 (RPKM 5.9), CNS E11.5 (RPKM 5.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Dars2 in Genome Data Viewer
Location:
1 H2.1; 1 69.75 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (160868171..160898236, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (161037580..161070668, complement)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA, C/D box 47 Neighboring gene predicted gene, 25789 Neighboring gene growth arrest specific 5 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:163000837-163001115 Neighboring gene STARR-positive B cell enhancer ABC_E11097 Neighboring gene centromere protein L Neighboring gene kelch-like 20 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:163061431-163061751

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Neuronal ablation of mt-AspRS in mice induces immune pathway activation prior to severe and progressive cortical and behavioral disruption. Nemeth CL, et al. Exp Neurol, 2020 Apr. PMID 31887305, Free PMC Article
  2. Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Dogan SA, et al. Cell Metab, 2014 Mar 4. PMID 24606902
  3. Adaptation to mitochondrial stress requires CHOP-directed tuning of ISR. Kaspar S, et al. Sci Adv, 2021 May. PMID 34039602, Free PMC Article
  4. DARS2 is indispensable for Purkinje cell survival and protects against cerebellar ataxia. Rumyantseva A, et al. Hum Mol Genet, 2020 Oct 10. PMID 32766765
  5. DARS2 protects against neuroinflammation and apoptotic neuronal loss, but is dispensable for myelin producing cells. Aradjanski M, et al. Hum Mol Genet, 2017 Nov 1. PMID 28985337

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. DARS2 is indispensable for Purkinje cell survival and protects against cerebellar ataxia.
    Title: DARS2 is indispensable for Purkinje cell survival and protects against cerebellar ataxia.
  2. Neuronal ablation of mt-AspRS in mice induces immune pathway activation prior to severe and progressive cortical and behavioral disruption.
    Title: Neuronal ablation of mt-AspRS in mice induces immune pathway activation prior to severe and progressive cortical and behavioral disruption.
  3. Results now provide the first evidence that loss of DARS2 in neurons leads to strong mitochondrial dysfunction and progressive loss of cells.
    Title: DARS2 protects against neuroinflammation and apoptotic neuronal loss, but is dispensable for myelin producing cells.
  4. DARS2 depletion in heart and skeletal muscle leads to the severe deregulation of mitochondrial protein synthesis followed by a strong respiratory chain deficit in both tissues.
    Title: Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Targeted (3)  1 citation

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC99963

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aspartate-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables aspartate-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables aspartate-tRNA ligase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables aspartate-tRNA(Asn) ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables aspartate-tRNA(Asn) ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in aspartyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitochondrial asparaginyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in mitochondrial asparaginyl-tRNA aminoacylation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in mitochondrial matrix ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial matrix ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in mitochondrial membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
aspartate--tRNA ligase, mitochondrial
NP_766232.1
XP_006496820.1
XP_006496821.1
XP_036019957.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_172644.4 → NP_766232.1  aspartate--tRNA ligase, mitochondrial precursor

    See identical proteins and their annotated locations for NP_766232.1

    Status: VALIDATED

    Source sequence(s)
    AK030952, AK138809, BY781897
    Consensus CDS
    CCDS15413.1
    UniProtKB/Swiss-Prot
    Q5FWV4, Q8BIP0
    Related
    ENSMUSP00000041851.4, ENSMUST00000035430.4
    Conserved Domains (4) summary
    PRK00476
    Location:49 → 636
    aspS; aspartyl-tRNA synthetase; Validated
    cd00777
    Location:186 → 606
    AspRS_core; Asp tRNA synthetase (aspRS) class II core domain. Class II assignment is based upon its structure and the presence of three characteristic sequence motifs. AspRS is a homodimer, which attaches a specific amino acid to the 3' OH group of ribose of the ...
    cd04317
    Location:49 → 182
    EcAspRS_like_N; EcAspRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli aspartyl-tRNA synthetase (AspRS), the human mitochondrial (mt) AspRS-2, the discriminating (D) Thermus thermophilus AspRS-1, and the nondiscriminating (ND) ...
    pfam02938
    Location:360 → 451
    GAD; GAD domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    160868171..160898236 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006496757.3 → XP_006496820.1  aspartate--tRNA ligase, mitochondrial isoform X1

    Conserved Domains (1) summary
    PRK00476
    Location:49 → 615
    aspS; aspartyl-tRNA synthetase; Validated

  2. XM_036164064.1 → XP_036019957.1  aspartate--tRNA ligase, mitochondrial isoform X3

    Conserved Domains (1) summary
    PRK00476
    Location:1 → 483
    aspS; aspartyl-tRNA synthetase; Validated

  3. XM_006496758.4 → XP_006496821.1  aspartate--tRNA ligase, mitochondrial isoform X2

    Conserved Domains (1) summary
    PRK00476
    Location:1 → 504
    aspS; aspartyl-tRNA synthetase; Validated
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8BIP0.1 GenPept UniProtKB/Swiss-Prot:Q8BIP0

Gene LinkOut

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