Slc6a2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc6a2provided by MGI
Official Full Name: solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2provided by MGI
Primary source: MGI:MGI:1270850
See related: Ensembl:ENSMUSG00000055368 AllianceGenome:MGI:1270850
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NET; NE-T; Slc6a5
Summary: Enables neurotransmitter transmembrane transporter activity and norepinephrine:sodium symporter activity. Involved in neurotransmitter reuptake and norepinephrine uptake. Acts upstream of or within neurotransmitter transport and response to pain. Predicted to be located in cell surface. Predicted to be active in axon; neuronal cell body membrane; and presynaptic membrane. Is expressed in several structures, including adrenal gland; lip; nervous system; renal pelvis; and sensory organ. Used to study heart conduction disease. Human ortholog(s) of this gene implicated in alcohol dependence; hypertension; and neurocirculatory asthenia. Orthologous to human SLC6A2 (solute carrier family 6 member 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in placenta adult (RPKM 4.2), subcutaneous fat pad adult (RPKM 4.1) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C5; 8 44.99 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (93687100..93728295)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (92960470..93001667)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dual orexin receptor antagonist (DORA-12) treatment affects the overall levels of Net/maoA mRNA expression in the hippocampus.
Title: Dual orexin receptor antagonist (DORA-12) treatment affects the overall levels of Net/maoA mRNA expression in the hippocampus.
Differences in gene expression in the mouse frontal cortex with life-long norepinephrine transporter knock-out were studied using a whole-genome microarray approach
Title: Life-long norepinephrine transporter (NET) knock-out leads to the increase in the NET mRNA in brain regions rich in norepinephrine terminals.
inhibition of p38 MAPK or the manipulation of NET-Thr(30) motif/phosphorylation via a TAT peptide strategy prevents cocaine-induced NET up-regulation, locomotor sensitization, and conditioned place preference
Title: A Role for p38 Mitogen-activated Protein Kinase-mediated Threonine 30-dependent Norepinephrine Transporter Regulation in Cocaine Sensitization and Conditioned Place Preference.
this is the first report which suggests the beneficial effect of modulation of TRPV1 receptors and NET in Chronic cerebral hypoperfusion vascular dementia
Title: Modulation of transient receptor potential vanilloid subtype 1 (TRPV1) and norepinephrine transporters (NET) protect against oxidative stress, cellular injury, and vascular dementia.
daily sympathetic activation induced by mild chronic stress was unable to induce bone loss, unless NET activity was blocked.
Title: Extracellular norepinephrine clearance by the norepinephrine transporter is required for skeletal homeostasis.
NET dysfunction is sufficient to produce a postural orthostatic tachycardia syndrome phenotype.
Title: Norepinephrine transporter variant A457P knock-in mice display key features of human postural orthostatic tachycardia syndrome.
Norepinephrine transporter has a greater role in determining baseline analgesia and other analgesic effects in mice than serotonin transporter.
Title: A greater role for the norepinephrine transporter than the serotonin transporter in murine nociception.
NET expression is essential for development and/or survival of some (5-HT- and calretinin-expressing), but not all (nNOS-expressing), of these neurons.
Title: Dependence of serotonergic and other nonadrenergic enteric neurons on norepinephrine transporter expression.
propose that amplified norepinephrine transporter function in rictor KO mice enhances accumulation of both norepinephrine and dopamine within the noradrenergic neuron
Title: Dysregulation of the norepinephrine transporter sustains cortical hypodopaminergia and schizophrenia-like behaviors in neuronal rictor null mice.
Report a protocol to monitor NET transport rate at the level of the single nerve terminal varicosity using a fluorescent substrate.
Title: Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Targeted (5) 1 citation

General gene information

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Markers

RH141588 (e-PCR)
- UniSTS:223582

Slc6a2 (e-PCR), detects polymorphism
- UniSTS:546022

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin binding	ISO Inferred from Sequence Orthology more info
enables alpha-tubulin binding	IEA Inferred from Electronic Annotation more info
enables alpha-tubulin binding	ISO Inferred from Sequence Orthology more info
enables beta-tubulin binding	IEA Inferred from Electronic Annotation more info
enables beta-tubulin binding	ISO Inferred from Sequence Orthology more info
enables dopamine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables dopamine:sodium symporter activity	IEA Inferred from Electronic Annotation more info
enables dopamine:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monoamine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables monoamine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info	PubMed
enables neurotransmitter transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables neurotransmitter:sodium symporter activity	IEA Inferred from Electronic Annotation more info
enables norepinephrine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables norepinephrine:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables norepinephrine:sodium symporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in amino acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in dopamine uptake	IEA Inferred from Electronic Annotation more info
involved_in dopamine uptake involved in synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within monoamine transport	ISO Inferred from Sequence Orthology more info	PubMed
involved_in neurotransmitter reuptake	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter reuptake	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neurotransmitter transport	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in norepinephrine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in norepinephrine transport	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine uptake	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to pain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in cell surface	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
is_active_in neuronal cell body membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in synaptic vesicle membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sodium-dependent noradrenaline transporter

Names: NE transporter; norepinephrine transporter; solute carrier family 6 member 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009209.3 → NP_033235.3 sodium-dependent noradrenaline transporter

See identical proteins and their annotated locations for NP_033235.3

Status: VALIDATED

Source sequence(s)

AC140217, AJ439987, AK078616, AY188506, CD551617

Consensus CDS

CCDS22525.1

UniProtKB/Swiss-Prot

O55192, Q6QU62

UniProtKB/TrEMBL

Q8R2I2

Related

ENSMUSP00000072709.7, ENSMUST00000072939.8

Conserved Domains (1) summary

cl00456
Location:56 → 615

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

93687100..93728295

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006530793.5 → XP_006530856.2 sodium-dependent noradrenaline transporter isoform X1

UniProtKB/Swiss-Prot

O55192, Q6QU62

UniProtKB/TrEMBL

Q8R2I2

Conserved Domains (1) summary

cl00456
Location:56 → 615

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain
XM_006530794.5 → XP_006530857.2 sodium-dependent noradrenaline transporter isoform X1

UniProtKB/Swiss-Prot

O55192, Q6QU62

UniProtKB/TrEMBL

Q8R2I2

Conserved Domains (1) summary

cl00456
Location:56 → 615

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain