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EIF5 eukaryotic translation initiation factor 5 [ Homo sapiens (human) ]

Gene ID: 1983, updated on 5-Mar-2024

Summary

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Official Symbol
EIF5provided by HGNC
Official Full Name
eukaryotic translation initiation factor 5provided by HGNC
Primary source
HGNC:HGNC:3299
See related
Ensembl:ENSG00000100664 MIM:601710; AllianceGenome:HGNC:3299
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
EIF-5; EIF-5A
Summary
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]
Expression
Ubiquitous expression in bone marrow (RPKM 60.5), thyroid (RPKM 44.5) and 25 other tissues See more
Orthologs
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Genomic context

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See EIF5 in Genome Data Viewer
Location:
14q32.32
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 14 NC_000014.9 (103334237..103345025)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 14 NC_060938.1 (97570092..97580884)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 14 NC_000014.8 (103800574..103811362)

Chromosome 14 - NC_000014.9Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105370686 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr14:103768183-103768683 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr14:103777212-103778188 Neighboring gene CRISPRi-validated cis-regulatory element chr14.2493 Neighboring gene uncharacterized LOC105378183 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9099 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9100 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9101 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6148 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6149 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6150 Neighboring gene EIF5 divergent transcript Neighboring gene small nucleolar RNA, H/ACA box 28 Neighboring gene Sharpr-MPRA regulatory region 8941 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr14:103827642-103828841 Neighboring gene high mobility group box 3 pseudogene 26 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr14:103838386-103839043 Neighboring gene ribosomal protein SA pseudogene 5

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5. Bieniossek C, et al. J Mol Biol, 2006 Jul 7. PMID 16781736
  2. Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors. Conte MR, et al. Biochemistry, 2006 Apr 11. PMID 16584190
  3. Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). Si K, et al. J Biol Chem, 1996 Jul 12. PMID 8663286
  4. A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer. Humphries MP, et al. Clin Cancer Res, 2017 May 15. PMID 27986751
  5. Sliding of a 43S ribosomal complex from the recognized AUG codon triggered by a delay in eIF2-bound GTP hydrolysis. Terenin IM, et al. Nucleic Acids Res, 2016 Feb 29. PMID 26717981, Free PMC Article

See all (106) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Roles of HDAC2, eIF5, and eIF6 in Lung Cancer Tumorigenesis.
    Title: Roles of HDAC2, eIF5, and eIF6 in Lung Cancer Tumorigenesis.
  2. Translational initiation pathway inhibition could be of clinical utility in male breast cancer patients overexpressing eIF4E and eIF5. With mTOR inhibitors that target this pathway now in the clinic, these biomarkers may represent new targets for therapeutic intervention, although further independent validation is required
    Title: A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer.
  3. Data show that eIF5-mimic protein (5MP) represses non-AUG translation by competing with translation initiation factor 5 (eIF5) for the Met-tRNAi-binding factor eIF2.
    Title: Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.
  4. The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF.
    Title: Defect in the GTPase activating protein (GAP) function of eIF5 causes repression of GCN4 translation.
  5. overexpression of eIF5 and 5MP induces translation of ATF4
    Title: Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.
  6. it is eIF5-induced GTP hydrolysis and Pi release that irreversibly trap the 48S complex, and this complex is further stabilized by eIF5B and 60S joining.
    Title: Sliding of a 43S ribosomal complex from the recognized AUG codon triggered by a delay in eIF2-bound GTP hydrolysis.
  7. The N-terminal tail of eIF1A mediates the interaction with eIF5 and eIF1.
    Title: The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes.
  8. Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex
    Title: Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex.
  9. This study provides mechanistic insight into the role of eIF5-carboxyl terminal domain's dynamic interplay with eIF1 and eIF2beta.
    Title: The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β.
  10. miR-5787 represses cell growth, in part, by targeting eIF5.
    Title: The hsa-miR-5787 represses cellular growth by targeting eukaryotic translation initiation factor 5 (eIF5) in fibroblasts.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Seventy-five genetic loci influencing the human red blood cell.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag Cellular biotinylated eukaryotic translation initiation factor 5 (EIF5) protein is incorporated into HIV-1 Gag virus-like particles PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables GDP-dissociation inhibitor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activator activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding HDA PubMed 
enables cadherin binding HDA PubMed 
enables eukaryotic initiation factor eIF2 binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables translation initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in formation of cytoplasmic translation initiation complex IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of translational initiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ribosome assembly IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
NOT located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
  
located_in synapse IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
eukaryotic translation initiation factor 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001969.5 → NP_001960.2  eukaryotic translation initiation factor 5

    See identical proteins and their annotated locations for NP_001960.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AL138976
    Consensus CDS
    CCDS9980.1
    UniProtKB/Swiss-Prot
    P55010, Q53XB3, Q9H5N2, Q9UG48
    UniProtKB/TrEMBL
    Q6IBU0
    Related
    ENSP00000216554.3, ENST00000216554.8
    Conserved Domains (2) summary
    cd11561
    Location:233 → 385
    W2_eIF5; C-terminal W2 domain of eukaryotic translation initiation factor 5
    pfam01873
    Location:9 → 128
    eIF-5_eIF-2B; Domain found in IF2B/IF5

  2. NM_183004.5 → NP_892116.2  eukaryotic translation initiation factor 5

    See identical proteins and their annotated locations for NP_892116.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AL138976
    Consensus CDS
    CCDS9980.1
    UniProtKB/Swiss-Prot
    P55010, Q53XB3, Q9H5N2, Q9UG48
    UniProtKB/TrEMBL
    Q6IBU0
    Related
    ENSP00000376477.2, ENST00000392715.6
    Conserved Domains (2) summary
    cd11561
    Location:233 → 385
    W2_eIF5; C-terminal W2 domain of eukaryotic translation initiation factor 5
    pfam01873
    Location:9 → 128
    eIF-5_eIF-2B; Domain found in IF2B/IF5

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000014.9 Reference GRCh38.p14 Primary Assembly

    Range
    103334237..103345025
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060938.1 Alternate T2T-CHM13v2.0

    Range
    97570092..97580884
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P55010.2 GenPept UniProtKB/Swiss-Prot:P55010

Gene LinkOut

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