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Rag2 recombination activating gene 2 [ Mus musculus (house mouse) ]

Gene ID: 19374, updated on 12-Nov-2024

Summary

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Official Symbol
Rag2provided by MGI
Official Full Name
recombination activating gene 2provided by MGI
Primary source
MGI:MGI:97849
See related
Ensembl:ENSMUSG00000032864 AllianceGenome:MGI:97849
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Rag-2
Summary
Enables several functions, including phosphatidylinositol phosphate binding activity; phosphatidylinositol-3,4-bisphosphate binding activity; and zinc ion binding activity. Involved in T cell differentiation in thymus; V(D)J recombination; and pre-B cell allelic exclusion. Acts upstream of or within several processes, including B cell homeostatic proliferation; lymphocyte differentiation; and positive regulation of organ growth. Predicted to be located in nucleoplasm. Predicted to be part of DNA recombinase complex. Is expressed in gut; hemolymphoid system; and liver. Used to study Omenn syndrome and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, Nk cell-positive. Human ortholog(s) of this gene implicated in Omenn syndrome; combined cellular and humoral immune defects with granulomas; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, Nk cell-positive. Orthologous to human RAG2 (recombination activating 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Restricted expression toward thymus adult (RPKM 9.1) See more
Orthologs
human all
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Genomic context

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See Rag2 in Genome Data Viewer
Location:
2 E2; 2 53.87 cM
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (101455057..101462873)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (101624712..101632528)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene, 39886 Neighboring gene STARR-positive B cell enhancer mm9_chr2:101312844-101313145 Neighboring gene STARR-seq mESC enhancer starr_05230 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:101391576-101391907 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:101392526-101392727 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:101403351-101403564 Neighboring gene predicted gene, 39887 Neighboring gene intraflagellar transport associated protein Neighboring gene VISTA enhancer mm1328 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:101448688-101449009 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:101464796-101464979 Neighboring gene recombination activating 1 Neighboring gene predicted gene, 34041

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Rag2 Deficiency Enhances Susceptibility to Systemic Mouse Adenovirus Type 1 Infection. Lee HK, et al. Intervirology, 2022. PMID 34736262, Free PMC Article
  2. Tumor-associated high endothelial venules mediate lymphocyte entry into tumors and predict response to PD-1 plus CTLA-4 combination immunotherapy. Asrir A, et al. Cancer Cell, 2022 Mar 14. PMID 35120598
  3. Pro-B cells propagated in stromal cell-free cultures reconstitute functional B-cell compartments in immunodeficient mice. von Muenchow L, et al. Eur J Immunol, 2017 Feb. PMID 27925658
  4. Analysis of regions of RAG-2 important for V(D)J recombination. Cuomo CA, et al. Nucleic Acids Res, 1994 May 25. PMID 8208604, Free PMC Article
  5. Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch. Kondo E, et al. Int Immunol, 2003 Mar. PMID 12618483

See all (1000) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Natural Killer Cells Do Not Attenuate a Mouse-Adapted SARS-CoV-2-Induced Disease in Rag2[-/-] Mice.
    Title: Natural Killer Cells Do Not Attenuate a Mouse-Adapted SARS-CoV-2-Induced Disease in Rag2(-/-) Mice.
  2. Hyperactive Natural Killer cells in Rag2 knockout mice inhibit the development of acute myeloid leukemia.
    Title: Hyperactive Natural Killer cells in Rag2 knockout mice inhibit the development of acute myeloid leukemia.
  3. Rag2(-/-) accelerates lipofuscin accumulation in the brain: Implications for human stem cell brain transplantation studies.
    Title: Rag2(-/-) accelerates lipofuscin accumulation in the brain: Implications for human stem cell brain transplantation studies.
  4. Rag2 Deficiency Enhances Susceptibility to Systemic Mouse Adenovirus Type 1 Infection.
    Title: Rag2 Deficiency Enhances Susceptibility to Systemic Mouse Adenovirus Type 1 Infection.
  5. PTPN2 links colonic and joint inflammation in experimental autoimmune arthritis.
    Title: PTPN2 links colonic and joint inflammation in experimental autoimmune arthritis.
  6. GITR controls intestinal inflammation by suppressing IL-15-dependent NK cell activity.
    Title: GITR controls intestinal inflammation by suppressing IL-15-dependent NK cell activity.
  7. Loop extrusion mediates physiological Igh locus contraction for RAG scanning.
    Title: Loop extrusion mediates physiological Igh locus contraction for RAG scanning.
  8. Male-Dependent Promotion of Colitis in 129 Rag2(-/-) Mice Co-Infected with Helicobacter pylori and Helicobacter hepaticus.
    Title: Male-Dependent Promotion of Colitis in 129 Rag2(-/-) Mice Co-Infected with Helicobacter pylori and Helicobacter hepaticus.
  9. RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growth.
    Title: RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growth.
  10. Stress hormones or general well-being are not altered in immune-deficient mice lacking either T- and B- lymphocytes or Interferon gamma signaling if kept under specific pathogen free housing conditions.
    Title: Stress hormones or general well-being are not altered in immune-deficient mice lacking either T- and B- lymphocytes or Interferon gamma signaling if kept under specific pathogen free housing conditions.
Submit: New GeneRIF Correction See all GeneRIFs (108)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylated histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-3,4,5-trisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-3,4-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-3,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-4,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within B cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in B cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within B cell homeostatic proliferation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within B cell lineage commitment IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DN2 thymocyte differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA recombination TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within T cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in T cell differentiation in thymus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within T cell lineage commitment IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in V(D)J recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within V(D)J recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in V(D)J recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chromatin organization IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within defense response to bacterium IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mature B cell differentiation involved in immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in mature B cell differentiation involved in immune response ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of T cell differentiation in thymus IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of T cell differentiation in thymus ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of organ growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of organ growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of organ growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within positive regulation of organ growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pre-B cell allelic exclusion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA recombinase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IC
Inferred by Curator
more info
 PubMed 

General protein information

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Preferred Names
V(D)J recombination-activating protein 2
Names
recombinase activating gene 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_009020.4 → NP_033046.1  V(D)J recombination-activating protein 2

    See identical proteins and their annotated locations for NP_033046.1

    Status: VALIDATED

    Source sequence(s)
    AK040375, BB654295
    Consensus CDS
    CCDS16462.1
    UniProtKB/Swiss-Prot
    P21784
    UniProtKB/TrEMBL
    Q542D1, Q80Z95
    Related
    ENSMUSP00000038204.4, ENSMUST00000044031.4
    Conserved Domains (2) summary
    pfam03089
    Location:51 → 388
    RAG2; Recombination activating protein 2
    pfam13341
    Location:414 → 491
    RAG2_PHD; RAG2 PHD domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    101455057..101462873
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017316606.3 → XP_017172095.1  V(D)J recombination-activating protein 2 isoform X1

    UniProtKB/Swiss-Prot
    P21784
    UniProtKB/TrEMBL
    Q542D1, Q80Z95
    Conserved Domains (2) summary
    pfam03089
    Location:51 → 388
    RAG2; Recombination activating protein 2
    pfam13341
    Location:414 → 491
    RAG2_PHD; RAG2 PHD domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P21784.2 GenPept UniProtKB/Swiss-Prot:P21784

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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