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Pknox1 Pbx/knotted 1 homeobox [ Mus musculus (house mouse) ]

Gene ID: 18771, updated on 14-Nov-2024

Summary

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Official Symbol
Pknox1provided by MGI
Official Full Name
Pbx/knotted 1 homeoboxprovided by MGI
Primary source
MGI:MGI:1201409
See related
Ensembl:ENSMUSG00000006705 AllianceGenome:MGI:1201409
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PREP1; D17Wsu76e
Summary
Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Acts upstream of or within several processes, including angiogenesis; hemopoiesis; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of transcription regulator complex. Is expressed in several structures, including alimentary system; branchial arch; genitourinary system; nervous system; and sensory organ. Orthologous to human PKNOX1 (PBX/knotted 1 homeobox 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in thymus adult (RPKM 12.1), testis adult (RPKM 10.4) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Pknox1 in Genome Data Viewer
Location:
17 B1; 17 16.78 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (31783697..31826665)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (31564722..31607693)

Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E3197 Neighboring gene NADH:ubiquinone oxidoreductase core subunit V3 Neighboring gene predicted gene, 50105 Neighboring gene STARR-positive B cell enhancer ABC_E9439 Neighboring gene STARR-seq mESC enhancer starr_42341 Neighboring gene STARR-seq mESC enhancer starr_42343 Neighboring gene cystathionine beta-synthase Neighboring gene STARR-seq mESC enhancer starr_42345 Neighboring gene ribosomal protein S2, pseudogene 8

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging. Cimmino I, et al. FASEB J, 2021 Nov. PMID 34679197
  2. Pknox1/Prep1 regulates mitochondrial oxidative phosphorylation components in skeletal muscle. Kanzleiter T, et al. Mol Cell Biol, 2014 Jan. PMID 24216763, Free PMC Article
  3. Prep1 (pKnox1) regulates mouse embryonic HSC cycling and self-renewal affecting the Stat1-Sca1 IFN-dependent pathway. Modica L, et al. PLoS One, 2014. PMID 25233378, Free PMC Article
  4. ChIP-Seq and RNA-Seq analyses identify components of the Wnt and Fgf signaling pathways as Prep1 target genes in mouse embryonic stem cells. Laurent A, et al. PLoS One, 2015. PMID 25875616, Free PMC Article
  5. Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow. Yoshioka K, et al. PLoS One, 2015. PMID 26285139, Free PMC Article

See all (100) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
    Title: Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
  2. Prep1, complexing with p160, decreases PGC-1alpha and stimulates angiogenesis.
    Title: Prep1 regulates angiogenesis through a PGC-1α-mediated mechanism.
  3. Prep1 DR expands the DNA binding landscape of C/EBPb (CCAAT enhancer binding protein b) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPb.
    Title: Prep1 prevents premature adipogenesis of mesenchymal progenitors.
  4. We conclude that Prep1 is required for normal adult mammary gland development, in particular at its branching morphogenesis step. By binding Snail2, Prep1 protects it from the proteasomal degradation.
    Title: Prep1 (pKnox1) transcription factor contributes to pubertal mammary gland branching morphogenesis.
  5. a mechanism by which the DNA binding-independent activity of Sp2 potentiates genomic loading of Pbx1:Prep1 and Nf-y to composite motifs present in many promoters of highly expressed genes
    Title: Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites.
  6. Prep1 deficiency alters olfactory morpho-functional integrity and olfaction-mediated eating behavior by affecting BDNF-TrkB signaling.
    Title: Prep1 Deficiency Affects Olfactory Perception and Feeding Behavior by Impairing BDNF-TrkB Mediated Neurotrophic Signaling.
  7. Prep1 silencing reduces inflammatory response and increases insulin sensitivity in adipose tissue.
    Title: Prep1 deficiency improves metabolic response in white adipose tissue.
  8. results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes
    Title: Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
  9. However, Prep1 doesn't affect gluconeogenesis in the absence of insulin. Also, we observed that nuclear content of gluconeogenic transcription factor FOXO1 was greatly reduced in Prep1 knockout hepatocytes. These findings suggest that Prep1 may control hepatic insulin sensitivity by targeting FOXO1 nuclear stability.
    Title: The transcription factor Prep1 controls hepatic insulin sensitivity and gluconeogenesis by targeting nuclear localization of FOXO1.
  10. Study found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression
    Title: Transcriptional control of insulin-sensitive glucose carrier Glut4 expression in adipose tissue cells.
Submit: New GeneRIF Correction See all GeneRIFs (36)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Gene trapped (4) 
  • Targeted (7)  1 citation

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific double-stranded DNA binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within T cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in angiogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within angiogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within camera-type eye development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within erythrocyte differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within hemopoiesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
part_of transcription regulator complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
homeobox protein PKNOX1
Names
PBX/knotted homeobox 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001411456.1 → NP_001398385.1  homeobox protein PKNOX1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/Swiss-Prot
    O70477, Q7TT01

  2. NM_001411457.1 → NP_001398386.1  homeobox protein PKNOX1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/Swiss-Prot
    O70477, Q7TT01

  3. NM_001411458.1 → NP_001398387.1  homeobox protein PKNOX1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/Swiss-Prot
    O70477, Q7TT01

  4. NM_001411459.1 → NP_001398388.1  homeobox protein PKNOX1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/Swiss-Prot
    O70477, Q7TT01

  5. NM_001411460.1 → NP_001398389.1  homeobox protein PKNOX1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/TrEMBL
    A0A494B9U0
    Related
    ENSMUSP00000157748.2, ENSMUST00000237248.2

  6. NM_001411461.1 → NP_001398390.1  homeobox protein PKNOX1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/TrEMBL
    A0A494B9U0

  7. NM_001411462.1 → NP_001398391.1  homeobox protein PKNOX1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/TrEMBL
    A0A494B9U0

  8. NM_001411463.1 → NP_001398392.1  homeobox protein PKNOX1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/TrEMBL
    A0A494B9U0

  9. NM_001411464.1 → NP_001398393.1  homeobox protein PKNOX1 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575

  10. NM_001411465.1 → NP_001398394.1  homeobox protein PKNOX1 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575

  11. NM_001411466.1 → NP_001398395.1  homeobox protein PKNOX1 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575

  12. NM_001411467.1 → NP_001398396.1  homeobox protein PKNOX1 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575

  13. NM_001411468.1 → NP_001398397.1  homeobox protein PKNOX1 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    Related
    ENSMUSP00000157491.2, ENSMUST00000237038.2

  14. NM_001411469.1 → NP_001398398.1  homeobox protein PKNOX1 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575

  15. NM_001411864.1 → NP_001398793.1  homeobox protein PKNOX1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC166172, AC166575
    UniProtKB/Swiss-Prot
    O70477, Q7TT01
    Related
    ENSMUSP00000134852.2, ENSMUST00000175806.9

  16. NM_016670.4 → NP_057879.2  homeobox protein PKNOX1 isoform 1

    See identical proteins and their annotated locations for NP_057879.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the functional protein.
    Source sequence(s)
    AC166172, AC166575
    Consensus CDS
    CCDS28608.1
    UniProtKB/Swiss-Prot
    O70477, Q7TT01
    Related
    ENSMUSP00000094966.4, ENSMUST00000097352.11
    Conserved Domains (2) summary
    pfam05920
    Location:277 → 316
    Homeobox_KN; Homeobox KN domain
    pfam16493
    Location:80 → 165
    Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000083.7 Reference GRCm39 C57BL/6J

    Range
    31783697..31826665
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036160407.1 → XP_036016300.1  homeobox protein PKNOX1 isoform X4

    Conserved Domains (2) summary
    pfam05920
    Location:274 → 313
    Homeobox_KN; Homeobox KN domain
    pfam16493
    Location:80 → 165
    Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NR_027493.1: Suppressed sequence

    Description
    NR_027493.1: This RefSeq was removed because it is now thought that this transcript does encode a protein.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O70477.3 GenPept UniProtKB/Swiss-Prot:O70477

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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