Ndst2 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ndst2provided by MGI
Official Full Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2provided by MGI
Primary source: MGI:MGI:97040
See related: Ensembl:ENSMUSG00000039308 AllianceGenome:MGI:97040
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mndns; NDST-2
Summary: Enables N-acetylglucosamine deacetylase activity; [heparan sulfate]-glucosamine N-sulfotransferase activity; and heparan sulfate N-deacetylase activity. Involved in heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process. Acts upstream of or within mast cell mediated immunity and regulation of angiotensin levels in blood. Located in Golgi apparatus. Is expressed in several structures, including brain; genitourinary system; liver; lung; and spleen. Orthologous to human NDST2 (N-deacetylase and N-sulfotransferase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in ovary adult (RPKM 14.4), limb E14.5 (RPKM 12.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 A3; 14 11.53 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (20773798..20784638, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (20723730..20734570, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

An essential role for Heparin sulfate proteoglycan sulfation via NDST1,2,3 in modulating signal transduction during mouse implantation.
Title: Heparan Sulfate Proteoglycan Sulfation Regulates Uterine Differentiation and Signaling During Embryo Implantation.
Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content. A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length.
Title: NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length.
embryonic stem cells lacking both NDST1 and NDST2, expressing a very low sulfated heparan sulfate, can take the initial step toward differentiation into all three germ layers
Title: Undersulfation of heparan sulfate restricts differentiation potential of mouse embryonic stem cells.
expression of N-deacetylase/N-sulfotransferase (NDST)-2, a key enzyme in mast cell maturation
Title: Mast cell differentiation and activation is closely linked to expression of genes coding for the serglycin proteoglycan core protein and a distinct set of chondroitin sulfate and heparin sulfotransferases.
NDST1 and NDST2 have roles in N-sulfation but not in 6-O-sulfation of heparan sulfate
Title: Heparan sulfate synthesized by mouse embryonic stem cells deficient in NDST1 and NDST2 is 6-O-sulfated but contains no N-sulfate groups.
NDST-2 does not contribute significantly to tissue-specific Heparan Sulfate structures
Title: Heparan sulfate structure in mice with genetically modified heparan sulfate production.
although NDST-2(-/-) mice demonstrate an altered response in interstitial fulid pressure after mast cell activation, there was no similar alteration after neurogenic inflammation
Title: Neurogenic inflammation in mice deficient in heparin-synthesizing enzyme.
analysis of liver NDST1 and NDST2 biosynthesis
Title: Enzymatically active N-deacetylase/N-sulfotransferase-2 is present in liver but does not contribute to heparan sulfate N-sulfation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

General gene information

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Markers

Ndst2 (e-PCR), detects polymorphism
- UniSTS:225834

Ndst2 (e-PCR), detects polymorphism
- UniSTS:532561

GDB:642165 (e-PCR)
- UniSTS:158561

X75885 (e-PCR)
- UniSTS:125847

AA875438 (e-PCR)
- UniSTS:243373

Ndst2 (e-PCR), detects polymorphism
- UniSTS:547521

RH134514 (e-PCR)
- UniSTS:217794

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables N-acetylglucosamine deacetylase activity	IDA Inferred from Direct Assay more info	PubMed
enables [heparan sulfate]-glucosamine N-sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables [heparan sulfate]-glucosamine N-sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables [heparan sulfate]-glucosamine N-sulfotransferase activity	TAS Traceable Author Statement more info	PubMed
enables deacetylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables deacetylase activity	IDA Inferred from Direct Assay more info	PubMed
enables heparan sulfate N-deacetylase activity	IDA Inferred from Direct Assay more info	PubMed
enables sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within glycosaminoglycan biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in heparan sulfate proteoglycan biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within heparan sulfate proteoglycan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heparan sulfate proteoglycan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in heparin biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mast cell mediated immunity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of angiotensin levels in blood	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2

Names: N-HSST 2; N-heparan sulfate sulfotransferase 2; [Heparan sulfate]-glucosamine N-sulfotransferase; glucosaminyl N-deacetylase/N-sulfotransferase 2; glucosaminyl N-deacetylase/N-sulphotransferase 2; heparan N-sulfotransferase 2; heparan glucosaminyl N-deacetylase/N-sulfotransferase 2; heparan sulfate N-deacetylase/N-sulfotransferase 2; mastocytoma N-deacetylase/N-sulfotransferase

NP_034941.2

EC 2.8.2.8

XP_030103542.1

EC 2.8.2.8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010811.2 → NP_034941.2 bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2

See identical proteins and their annotated locations for NP_034941.2

Status: VALIDATED

Source sequence(s)

AC121599, AI173867, AK150100, BC110480

Consensus CDS

CCDS26854.1

UniProtKB/Swiss-Prot

P52850, Q3UDF4, Q549P5

Related

ENSMUSP00000153036.2, ENSMUST00000223679.2

Conserved Domains (2) summary

pfam00685
Location:603 → 852

Sulfotransfer_1; Sulfotransferase domain

pfam12062
Location:26 → 514

HSNSD; heparan sulfate-N-deacetylase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

20773798..20784638 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030247682.1 → XP_030103542.1 bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X1

Conserved Domains (2) summary

pfam00685
Location:603 → 797

Sulfotransfer_1; Sulfotransferase domain

pfam12062
Location:26 → 514

HSNSD; heparan sulfate-N-deacetylase