png-1 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: png-1
Official Full Name: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Primary source: WormBase:WBGene00010160
Locus tag: CELE_F56G4.5
See related: AllianceGenome:WB:WBGene00010160
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity and protein-disulfide reductase (NAD(P)H) activity. Involved in ERAD pathway; cell redox homeostasis; and negative regulation of collateral sprouting. Located in cytoplasm. Is expressed in several structures, including body wall musculature; intestine; pharynx; rectal gland cell; and vulva. Human ortholog(s) of this gene implicated in congenital disorder of deglycosylation 1. Orthologous to human NGLY1 (N-glycanase 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See png-1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 10

Sequence:: Chromosome: I; NC_003279.8 (11380231..11388751)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutations in nucleotide metabolism genes bypass proteasome defects in png-1/NGLY1-deficient Caenorhabditis elegans.
Title: Mutations in nucleotide metabolism genes bypass proteasome defects in png-1/NGLY1-deficient Caenorhabditis elegans.
NGLY1: insights from Caenorhabditis elegans.
Title: NGLY1: insights from Caenorhabditis elegans.
Caenorhabditis elegans PNG-1 exhibits dual enzyme functions, not only as PNGase but also as an oxidoreductase (thioredoxin).
Title: Dual enzymatic properties of the cytoplasmic peptide: N-glycanase in C. elegans.
Authors show a developmental role for a PNGase and Rad-23 in the regulation of neuronal branching during organ innervation.
Title: The N-glycanase png-1 acts to limit axon branching during organ formation in Caenorhabditis elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F56G4.5 (e-PCR)
- UniSTS:322185

Y53H1C.a (e-PCR)
- UniSTS:324092

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IEA Inferred from Electronic Annotation more info
enables protein-disulfide reductase (NAD(P)H) activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-disulfide reductase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell redox homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycoprotein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycoprotein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycoprotein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of collateral sprouting	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

NP_492913.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

11380231..11388751

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_060512.5 → NP_492913.1 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_492913.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9NBD6, Q9TW67

Conserved Domains (4) summary

COG1305
Location:235 → 312

YebA; Transglutaminase-like enzyme, putative cysteine protease [Posttranslational modification, protein turnover, chaperones]

cd02947
Location:20 → 103

TRX_family; TRX family; composed of two groups: Group I, which includes proteins that exclusively encode a TRX domain; and Group II, which are composed of fusion proteins of TRX and additional domains. Group I TRX is a small ancient protein that alter the redox ...

pfam01841
Location:204 → 296

Transglut_core; Transglutaminase-like superfamily

cl10509
Location:407 → 604

PAW; PNGase C-terminal domain, mannose-binding module PAW