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Usp33 ubiquitin specific peptidase 33 [ Mus musculus (house mouse) ]

Gene ID: 170822, updated on 12-May-2024

Summary

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Official Symbol
Usp33provided by MGI
Official Full Name
ubiquitin specific peptidase 33provided by MGI
Primary source
MGI:MGI:2159711
See related
Ensembl:ENSMUSG00000025437 AllianceGenome:MGI:2159711
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Vdu1; 9830169D19Rik
Summary
Predicted to enable several functions, including cysteine-type peptidase activity; small GTPase binding activity; and zinc ion binding activity. Involved in axon guidance and cell migration. Located in cell body. Is expressed in central nervous system and retina. Orthologous to human USP33 (ubiquitin specific peptidase 33). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in CNS E18 (RPKM 26.3), CNS E14 (RPKM 24.1) and 25 other tissues See more
Orthologs
human all
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Genomic context

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See Usp33 in Genome Data Viewer
Location:
3 H3; 3 77.15 cM
Exon count:
25
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (152051797..152099254)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (152346184..152393617)

Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 31999 Neighboring gene VISTA enhancer mm72 Neighboring gene mitoguardin 1 Neighboring gene zinc finger, ZZ domain containing 3 Neighboring gene predicted gene, 57837 Neighboring gene RIKEN cDNA 3110067G11 gene Neighboring gene STARR-positive B cell enhancer ABC_E4606 Neighboring gene adenylate kinase 5

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Midline crossing and Slit responsiveness of commissural axons require USP33. Yuasa-Kawada J, et al. Nat Neurosci, 2009 Sep. PMID 19684588, Free PMC Article
  2. The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination. Cubillos-Rojas M, et al. Oncotarget, 2016 Aug 30. PMID 27528230, Free PMC Article
  3. Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2. Chan NC, et al. J Biol Chem, 2014 Jul 11. PMID 24855649, Free PMC Article
  4. Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein. Li Z, et al. J Biol Chem, 2002 Feb 15. PMID 11739384
  5. Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration. Yuasa-Kawada J, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706539, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.
    Title: miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.
  2. Knockdown or chemical inhibition of p97 causes robust accumulation of USP33 due to inhibition of its degradation
    Title: Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
  3. Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling
    Title: Midline crossing and Slit responsiveness of commissural axons require USP33.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (4) 
  • Targeted (1) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables G protein-coupled receptor binding ISO
Inferred from Sequence Orthology
more info
  
enables cysteine-type deubiquitinase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables cysteine-type endopeptidase activity ISO
Inferred from Sequence Orthology
more info
  
enables cysteine-type peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables small GTPase binding ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in axon guidance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to starvation ISO
Inferred from Sequence Orthology
more info
  
involved_in centrosome duplication ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of protein binding ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of protein binding ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K48-linked deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K63-linked deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein deubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein stabilization ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of G protein-coupled receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of autophagy ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in centrosome ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in focal adhesion ISO
Inferred from Sequence Orthology
more info
  
located_in neuronal cell body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
ubiquitin carboxyl-terminal hydrolase 33
Names
VHL-interacting deubiquitinating enzyme 1
Vhlh-interacting deubiquitinating enzyme 1
deubiquitinating enzyme 33
pVHL-interacting deubiquitinating enzyme 1
ubiquitin specific protease 33
ubiquitin thioesterase 33
ubiquitin thiolesterase 33
ubiquitin-specific-processing protease 33
NP_001070144.1
NP_001239415.1
NP_001342595.1
NP_573510.2
XP_030108303.1
XP_030108304.1
XP_036018806.1
XP_036018807.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001076676.2 → NP_001070144.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1, resulting in a shorter protein (isoform 2).
    Source sequence(s)
    AA210301, AK082943, AK087467
    Consensus CDS
    CCDS80064.1
    UniProtKB/TrEMBL
    A0A0H2UKB8, Q80TK2
    Related
    ENSMUSP00000026507.8, ENSMUST00000026507.13
    Conserved Domains (6) summary
    smart00695
    Location:799 → 879
    DUSP; Domain in ubiquitin-specific proteases
    cd02674
    Location:441 → 672
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    cl02553
    Location:155 → 287
    Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
    pfam00443
    Location:153 → 671
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:693 → 762
    DUSP; DUSP domain

  2. NM_001252486.1 → NP_001239415.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1, resulting in a shorter protein (isoform 3).
    Source sequence(s)
    AA210301, AK087467, AK143844, BC089315
    Consensus CDS
    CCDS57261.1
    UniProtKB/TrEMBL
    Q80TK2
    Related
    ENSMUSP00000113265.4, ENSMUST00000117492.9
    Conserved Domains (6) summary
    smart00695
    Location:799 → 883
    DUSP; Domain in ubiquitin-specific proteases
    cd02674
    Location:441 → 672
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    cl02553
    Location:155 → 287
    Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
    pfam00443
    Location:153 → 671
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:693 → 762
    DUSP; DUSP domain

  3. NM_001355666.1 → NP_001342595.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AA210301, AC122529
    UniProtKB/TrEMBL
    Q80TK2
    Conserved Domains (3) summary
    pfam00443
    Location:154 → 679
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:701 → 764
    DUSP; DUSP domain

  4. NM_133247.3 → NP_573510.2  ubiquitin carboxyl-terminal hydrolase 33 isoform 1

    See identical proteins and their annotated locations for NP_573510.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AA210301, AK087467, AK143844, BC089315
    Consensus CDS
    CCDS17918.1
    UniProtKB/Swiss-Prot
    Q3UP32, Q5FWK0, Q8K0I3, Q8R5K2, Q99K22
    UniProtKB/TrEMBL
    Q80TK2
    Related
    ENSMUSP00000142708.2, ENSMUST00000197748.5
    Conserved Domains (4) summary
    smart00695
    Location:807 → 891
    DUSP; Domain in ubiquitin-specific proteases
    pfam00443
    Location:154 → 679
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:701 → 770
    DUSP; DUSP domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000069.7 Reference GRCm39 C57BL/6J

    Range
    152051797..152099254
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036162913.1 → XP_036018806.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X2

    UniProtKB/TrEMBL
    Q80TK2
    Conserved Domains (3) summary
    pfam00443
    Location:154 → 679
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:701 → 770
    DUSP; DUSP domain

  2. XM_036162914.1 → XP_036018807.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X3

    UniProtKB/TrEMBL
    Q80TK2
    Conserved Domains (3) summary
    pfam00443
    Location:154 → 671
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:693 → 762
    DUSP; DUSP domain

  3. XM_030252444.2 → XP_030108304.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X4

    UniProtKB/TrEMBL
    Q80TK2
    Conserved Domains (3) summary
    pfam00443
    Location:154 → 671
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:693 → 756
    DUSP; DUSP domain

  4. XM_030252443.2 → XP_030108303.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X1

    UniProtKB/Swiss-Prot
    Q3UP32, Q5FWK0, Q8K0I3, Q8R5K2, Q99K22
    UniProtKB/TrEMBL
    Q80TK2
    Conserved Domains (4) summary
    smart00695
    Location:807 → 891
    DUSP; Domain in ubiquitin-specific proteases
    pfam00443
    Location:154 → 679
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam02148
    Location:30 → 90
    zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
    cl12116
    Location:701 → 770
    DUSP; DUSP domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8R5K2.2 GenPept UniProtKB/Swiss-Prot:Q8R5K2

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