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Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Mus musculus (house mouse) ]

Gene ID: 170768, updated on 9-Nov-2024

Summary

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Official Symbol
Pfkfb3provided by MGI
Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3provided by MGI
Primary source
MGI:MGI:2181202
See related
Ensembl:ENSMUSG00000026773 AllianceGenome:MGI:2181202
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
iPFK-2; uPFK-2; E330010H22Rik
Summary
Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Acts upstream of or within several processes, including glial cell differentiation; glycolytic process; and motor behavior. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; integumental system; nervous system; sensory organ; and skeleton. Used to study extrinsic cardiomyopathy. Orthologous to human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Broad expression in subcutaneous fat pad adult (RPKM 57.0), genital fat pad adult (RPKM 44.9) and 22 other tissues See more
Orthologs
human all
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Genomic context

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See Pfkfb3 in Genome Data Viewer
Location:
2 A1; 2 8.73 cM
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (11476241..11558882, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (11471430..11554071, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene predicted gene, 31990 Neighboring gene STARR-positive B cell enhancer ABC_E5882 Neighboring gene STARR-seq mESC enhancer starr_03667 Neighboring gene predicted gene, 52500 Neighboring gene predicted gene 10851 Neighboring gene STARR-seq mESC enhancer starr_03668 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:11470208-11470361 Neighboring gene STARR-positive B cell enhancer mm9_chr2:11475523-11475823 Neighboring gene STARR-seq mESC enhancer starr_03673 Neighboring gene STARR-positive B cell enhancer ABC_E5883 Neighboring gene RNA binding motif protein 17 Neighboring gene proteasome (prosome, macropain) 26S subunit, ATPase, 6 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. IRF8 and BATF3 interaction enhances the cDC1 specific Pfkfb3 gene expression. Chauhan KS, et al. Cell Immunol, 2022 Jan. PMID 34968772
  2. Pfkfb3 is transcriptionally upregulated in diabetic mouse liver through proliferative signals. Duran J, et al. FEBS J, 2009 Aug. PMID 19645723
  3. Disruption of inducible 6-phosphofructo-2-kinase ameliorates diet-induced adiposity but exacerbates systemic insulin resistance and adipose tissue inflammatory response. Huo Y, et al. J Biol Chem, 2010 Feb 5. PMID 19948719, Free PMC Article
  4. Exercise-induced re-programming of age-related metabolic changes in microglia is accompanied by a reduction in senescent cells. Mela V, et al. Brain Behav Immun, 2020 Jul. PMID 31978523
  5. Inhibition of PFKFB3 suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss. Wang J, et al. J Cell Mol Med, 2020 Feb. PMID 31880389, Free PMC Article

See all (104) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PFKFB3 ameliorates ischemia-induced neuronal damage by reducing reactive oxygen species and inhibiting nuclear translocation of Cdk5.
    Title: PFKFB3 ameliorates ischemia-induced neuronal damage by reducing reactive oxygen species and inhibiting nuclear translocation of Cdk5.
  2. The glycolytic enzyme PFKFB3 drives kidney fibrosis through promoting histone lactylation-mediated NF-kappaB family activation.
    Title: The glycolytic enzyme PFKFB3 drives kidney fibrosis through promoting histone lactylation-mediated NF-κB family activation.
  3. Hypoxia activates macrophage-NLRP3 inflammasome promoting atherosclerosis via PFKFB3-driven glycolysis.
    Title: Hypoxia activates macrophage-NLRP3 inflammasome promoting atherosclerosis via PFKFB3-driven glycolysis.
  4. MiR-106a-5p targets PFKFB3 and improves sepsis through regulating macrophage pyroptosis and inflammatory response.
    Title: MiR-106a-5p targets PFKFB3 and improves sepsis through regulating macrophage pyroptosis and inflammatory response.
  5. Expression of Pfkfb isoenzymes during in vitro differentiation of mouse embryonic stem cells into insulin-producing cells.
    Title: Expression of Pfkfb isoenzymes during in vitro differentiation of mouse embryonic stem cells into insulin-producing cells.
  6. Cardiac-specific PFKFB3 overexpression prevents diabetic cardiomyopathy via enhancing OPA1 stabilization mediated by K6-linked ubiquitination.
    Title: Cardiac-specific PFKFB3 overexpression prevents diabetic cardiomyopathy via enhancing OPA1 stabilization mediated by K6-linked ubiquitination.
  7. PFKFB3-mediated glycolysis in hepatic stellate cells promotes liver regeneration.
    Title: PFKFB3-mediated glycolysis in hepatic stellate cells promotes liver regeneration.
  8. Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
    Title: Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
  9. IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1alpha/PFKFB3 pathway.
    Title: IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1α/PFKFB3 pathway.
  10. PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1alpha Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
    Title: PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1α Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
Submit: New GeneRIF Correction See all GeneRIFs (60)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 2,6-bisphosphate metabolic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within glial cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within glycolytic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within motor behavior IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Names
inducible 6-phosphofructo-2-kinase
NP_001171223.1
NP_001171224.1
NP_001171225.1
NP_001171226.1
NP_001171227.1
NP_001171228.1
NP_001171229.1
NP_573495.2
XP_006497430.1
XP_006497432.1
XP_017171443.1
XP_030103781.1
XP_030103790.1
XP_036014832.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001177752.1 → NP_001171223.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 1

    See identical proteins and their annotated locations for NP_001171223.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) lacks 4 exons in the 3' coding region, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034675
    Consensus CDS
    CCDS50498.1
    UniProtKB/TrEMBL
    A2AUP3, A7UAK4, V9GWY6
    Related
    ENSMUSP00000138893.3, ENSMUST00000183869.8
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  2. NM_001177753.1 → NP_001171224.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2

    See identical proteins and their annotated locations for NP_001171224.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034676
    Consensus CDS
    CCDS50501.1
    UniProtKB/TrEMBL
    A2AUP4, A7UAK5
    Related
    ENSMUSP00000142079.2, ENSMUST00000191668.6
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  3. NM_001177754.1 → NP_001171225.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 3

    See identical proteins and their annotated locations for NP_001171225.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (3) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034677
    Consensus CDS
    CCDS79731.1
    UniProtKB/TrEMBL
    A2AUP3, A2AUP5, A7UAK6
    Related
    ENSMUSP00000050926.8, ENSMUST00000049849.12
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  4. NM_001177755.1 → NP_001171226.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 4

    See identical proteins and their annotated locations for NP_001171226.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034678
    Consensus CDS
    CCDS79732.1
    UniProtKB/TrEMBL
    A2AUP4, Q3U3S6
    Related
    ENSMUSP00000137130.3, ENSMUST00000179584.8
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  5. NM_001177756.1 → NP_001171227.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 5

    See identical proteins and their annotated locations for NP_001171227.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) lacks one alternate exon in the 3' coding region, compared to variant 2. The resulting isoform (5) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034679
    Consensus CDS
    CCDS50500.1
    UniProtKB/TrEMBL
    A2AUP4, A7UAK8
    Related
    ENSMUSP00000126305.3, ENSMUST00000170196.9
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  6. NM_001177757.1 → NP_001171228.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 7

    See identical proteins and their annotated locations for NP_001171228.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7) lacks an alternate in-frame exon in the 3' coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778
    Consensus CDS
    CCDS50499.1
    UniProtKB/TrEMBL
    A2AUP3, Q7TS91
    Related
    ENSMUSP00000028114.7, ENSMUST00000028114.13
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  7. NM_001177758.1 → NP_001171229.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 8

    Status: VALIDATED

    Description
    Transcript Variant: This variant (8) represents use of an alternate promoter and 5' UTR, uses a distinct translation start codon, and lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (8) has a distinct N-terminus, lacks an internal segment, and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    AK143330, AL831794, BC052400, BP764506, EU034678
    Consensus CDS
    CCDS79733.1
    UniProtKB/TrEMBL
    A0A0A6YY64, Q9ESY2
    Related
    ENSMUSP00000142296.2, ENSMUST00000192949.6
    Conserved Domains (2) summary
    pfam00300
    Location:229 → 412
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:4 → 226
    6PF2K; 6-phosphofructo-2-kinase

  8. NM_133232.3 → NP_573495.2  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 6

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (6) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778
    Consensus CDS
    CCDS15683.1
    UniProtKB/TrEMBL
    A2AUP1, Q9ESY2
    Related
    ENSMUSP00000110495.3, ENSMUST00000114846.9
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    11476241..11558882 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036158939.1 → XP_036014832.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X4

    UniProtKB/TrEMBL
    A2AUP3
    Conserved Domains (2) summary
    pfam00300
    Location:229 → 415
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:4 → 226
    6PF2K; 6-phosphofructo-2-kinase

  2. XM_006497367.1 → XP_006497430.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X2

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:7 → 226
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:229 → 412
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  3. XM_030247921.2 → XP_030103781.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X1

    UniProtKB/TrEMBL
    A2AUP3
    Conserved Domains (2) summary
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  4. XM_006497369.4 → XP_006497432.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X5

    See identical proteins and their annotated locations for XP_006497432.1

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:1 → 207
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:210 → 393
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  5. XM_030247930.1 → XP_030103790.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X3

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:9 → 228
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:231 → 414
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  6. XM_017315954.3 → XP_017171443.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X6

    UniProtKB/TrEMBL
    Q8BVM1, Q9ESY2
    Conserved Domains (2) summary
    pfam01591
    Location:9 → 228
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:231 → 414
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_172976.1: Suppressed sequence

    Description
    NM_172976.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.
Nucleotide Protein Strain
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