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Atp8a1 ATPase phospholipid transporting 8A1 [ Mus musculus (house mouse) ]

Gene ID: 11980, updated on 2-Nov-2024

Summary

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Official Symbol
Atp8a1provided by MGI
Official Full Name
ATPase phospholipid transporting 8A1provided by MGI
Primary source
MGI:MGI:1330848
See related
Ensembl:ENSMUSG00000037685 AllianceGenome:MGI:1330848
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
APLT; Atp3a2; ClassI; B230107D19Rik
Summary
Enables phosphatidylserine flippase activity. Involved in several processes, including learning; regulation of membrane lipid distribution; and synaptic vesicle endocytosis. Located in cytoplasmic vesicle and organelle membrane. Is active in glutamatergic synapse and synaptic vesicle membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human ATP8A1 (ATPase phospholipid transporting 8A1). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Broad expression in lung adult (RPKM 16.4), cortex adult (RPKM 15.1) and 26 other tissues See more
Orthologs
human all
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Genomic context

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See Atp8a1 in Genome Data Viewer
Location:
5 C3.1; 5 36.15 cM
Exon count:
39
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (67775482..68004822, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (67618139..67847484, complement)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA C330024D21 gene Neighboring gene CRISPRi-validated cis-regulatory element for Bend4 and Bendr Neighboring gene STARR-seq mESC enhancer starr_13319 Neighboring gene STARR-seq mESC enhancer starr_13320 Neighboring gene STARR-seq mESC enhancer starr_13321 Neighboring gene shisa family member 3 Neighboring gene predicted gene 15478 Neighboring gene predicted gene, 46914 Neighboring gene STARR-positive B cell enhancer ABC_E1052 Neighboring gene predicted gene, 52816 Neighboring gene predicted gene 15477 Neighboring gene RIKEN cDNA 4930425K10 gene Neighboring gene predicted gene 5108

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Identification of an erythroid ATP-dependent aminophospholipid transporter. Soupene E, et al. Br J Haematol, 2006 May. PMID 16643453
  2. Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II). Paterson JK, et al. Biochemistry, 2006 Apr 25. PMID 16618126
  3. Isolation, sequencing, and functional analysis of the TATA-less murine ATPase II promoter and structural analysis of the ATPase II gene. Sobocki T, et al. Biochim Biophys Acta, 2007 Jan. PMID 17239457, Free PMC Article
  4. Identification and functional expression of four isoforms of ATPase II, the putative aminophospholipid translocase. Effect of isoform variation on the ATPase activity and phospholipid specificity. Ding J, et al. J Biol Chem, 2000 Jul 28. PMID 10801890
  5. ATP8a1, an IFT27 binding partner, is dispensable for spermatogenesis and male fertility. Yap YT, et al. Mol Reprod Dev, 2021 May. PMID 33821543

See all (48) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1.
    Title: Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1.
  2. Atp8a1 deletion increases the proliferative activity of hematopoietic stem cells by impairing PTEN function.
    Title: Atp8a1 deletion increases the proliferative activity of hematopoietic stem cells by impairing PTEN function.
  3. ATP8a1, an IFT27 binding partner, is dispensable for spermatogenesis and male fertility.
    Title: ATP8a1, an IFT27 binding partner, is dispensable for spermatogenesis and male fertility.
  4. ATP8A1 is cleaved by the cysteine protease calpain during apoptosis, and the cleavage is prevented indirectly by caspase inhibition, involving blockage of calcium influx into platelets and subsequent calpain activation.
    Title: Calpain cleaves phospholipid flippase ATP8A1 during apoptosis in platelets.
  5. In a sociability test, the Atp8a1+ mice displayed no preference for an encaged stranger mouse over a novel object, which is a characteristic autistic-like behavior. In sharp contrast, Atp8a1 (-/-) mice displayed a preference for a stranger mouse over the novel object, which is characteristic of neurotypical mouse behavior.
    Title: Aberrant hippocampal Atp8a1 levels are associated with altered synaptic strength, electrical activity, and autistic-like behavior.
  6. The data of this study strongly indicated that Atp8a1 plays a central role in the PM-APLT activity of some mammalian cells.
    Title: Atp8a1 deficiency is associated with phosphatidylserine externalization in hippocampus and delayed hippocampus-dependent learning.
  7. APLT has a role in macrophage-induced nitrosylation/oxidation plays an important role in cell clearance
    Title: Nitrosative stress inhibits the aminophospholipid translocase resulting in phosphatidylserine externalization and macrophage engulfment: implications for the resolution of inflammation.
  8. Such observations suggest tissue-specific differences in transcription initiation complex assembly and regulation of ATPase II gene expression.
    Title: Isolation, sequencing, and functional analysis of the TATA-less murine ATPase II promoter and structural analysis of the ATPase II gene.
  9. ATPase activity of the secretory granule Atp8a1 is activated by phospholipids binding to a specific site
    Title: Lipid specific activation of the murine P4-ATPase Atp8a1 (ATPase II).
  10. role in normal phospholipid distribution in the bilayer, and for normal binding, penetration, and signaling by the zona pellucida
    Title: A novel aminophospholipid transporter exclusively expressed in spermatozoa is required for membrane lipid asymmetry and normal fertilization.
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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (2) 
  • Targeted (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • KIAA4233

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATPase-coupled intramembrane lipid transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylserine flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables phosphatidylserine flippase activity ISO
Inferred from Sequence Orthology
more info
  
enables phosphatidylserine flippase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables phosphatidylserine floppase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in aminophospholipid translocation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in aminophospholipid translocation ISO
Inferred from Sequence Orthology
more info
  
involved_in aminophospholipid translocation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in learning IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phospholipid translocation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of phospholipid translocation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in synaptic vesicle endocytosis EXP
Inferred from Experiment
more info
 PubMed 
involved_in synaptic vesicle endocytosis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in synaptic vesicle endocytosis IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in synaptic vesicle endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi apparatus ISO
Inferred from Sequence Orthology
more info
  
located_in chromaffin granule membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
is_active_in glutamatergic synapse EXP
Inferred from Experiment
more info
 PubMed 
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IEP
Inferred from Expression Pattern
more info
 PubMed 
is_active_in glutamatergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in intracellular membrane-bounded organelle ISO
Inferred from Sequence Orthology
more info
  
located_in organelle membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of phospholipid-translocating ATPase complex ISO
Inferred from Sequence Orthology
more info
  
part_of phospholipid-translocating ATPase complex ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in synaptic vesicle membrane EXP
Inferred from Experiment
more info
 PubMed 
is_active_in synaptic vesicle membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in synaptic vesicle membrane IEP
Inferred from Expression Pattern
more info
 PubMed 
is_active_in synaptic vesicle membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in synaptic vesicle membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in trans-Golgi network IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
phospholipid-transporting ATPase IA
Names
ATPase 8A1, aminophospholipid transporter (APLT), class I
ATPase 8A1, p type
ATPase class I type 8A member 1
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
P4-ATPase flippase complex alpha subunit ATP8A1
chromaffin granule ATPase II
probable phospholipid-transporting ATPase IA
NP_001034088.1
NP_001271274.1
NP_033857.1
XP_006503766.1
XP_011239000.1
XP_017176112.1
XP_017176113.1
XP_036020643.1
XP_036020644.1
XP_036020645.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001038999.2 → NP_001034088.1  phospholipid-transporting ATPase IA isoform a

    See identical proteins and their annotated locations for NP_001034088.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes isoform a.
    Source sequence(s)
    AC123662, AK045367, AK151709, BC094235
    Consensus CDS
    CCDS39104.1
    UniProtKB/Swiss-Prot
    P70704, Q8BR88
    UniProtKB/TrEMBL
    A1L332
    Related
    ENSMUSP00000042215.9, ENSMUST00000037380.15
    Conserved Domains (6) summary
    TIGR01652
    Location:49 → 1086
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase
    pfam00122
    Location:106 → 191
    E1-E2_ATPase; E1-E2 ATPase
    pfam13246
    Location:486 → 581
    Cation_ATPase; Cation transport ATPase (P-type)
    pfam16209
    Location:38 → 100
    PhoLip_ATPase_N; Phospholipid-translocating ATPase N-terminal
    pfam16212
    Location:827 → 1079
    PhoLip_ATPase_C; Phospholipid-translocating P-type ATPase C-terminal
    cl21460
    Location:796 → 825
    HAD_like; Haloacid Dehalogenase-like Hydrolases

  2. NM_001284345.1 → NP_001271274.1  phospholipid-transporting ATPase IA isoform c

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an alternate exon and contains a different alternate exon in the 5' coding region, compared to variant 1. It encodes isoform c, which is of the same size but lacks an internal segment, compared to isoform a.
    Source sequence(s)
    AC123662, AK045367, AK141559, AK151709, BC094235
    Consensus CDS
    CCDS80295.1
    UniProtKB/TrEMBL
    A0A0M3HEP7, A1L332
    Related
    ENSMUSP00000072738.8, ENSMUST00000072971.13
    Conserved Domains (6) summary
    TIGR01652
    Location:49 → 1086
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase
    pfam00122
    Location:106 → 191
    E1-E2_ATPase; E1-E2 ATPase
    pfam13246
    Location:486 → 581
    Cation_ATPase; Cation transport ATPase (P-type)
    pfam16209
    Location:38 → 100
    PhoLip_ATPase_N; Phospholipid-translocating ATPase N-terminal
    pfam16212
    Location:827 → 1079
    PhoLip_ATPase_C; Phospholipid-translocating P-type ATPase C-terminal
    cl21460
    Location:796 → 825
    HAD_like; Haloacid Dehalogenase-like Hydrolases

  3. NM_009727.3 → NP_033857.1  phospholipid-transporting ATPase IA isoform b

    See identical proteins and their annotated locations for NP_033857.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains an alternate exon and lacks two other alternate exons in the 5' coding region, compared to variant 1. It encodes isoform b, which is shorter than isoform a.
    Source sequence(s)
    AC123662, AK039919, AK045367, AK141559, AK151709, BC094235
    Consensus CDS
    CCDS39105.1
    UniProtKB/TrEMBL
    A1L332
    Related
    ENSMUSP00000118379.3, ENSMUST00000135930.8
    Conserved Domains (6) summary
    TIGR01652
    Location:49 → 1071
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase
    pfam00122
    Location:106 → 191
    E1-E2_ATPase; E1-E2 ATPase
    pfam13246
    Location:471 → 566
    Cation_ATPase; Cation transport ATPase (P-type)
    pfam16209
    Location:38 → 100
    PhoLip_ATPase_N; Phospholipid-translocating ATPase N-terminal
    pfam16212
    Location:812 → 1064
    PhoLip_ATPase_C; Phospholipid-translocating P-type ATPase C-terminal
    cl21460
    Location:781 → 810
    HAD_like; Haloacid Dehalogenase-like Hydrolases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    67775482..68004822 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503703.5 → XP_006503766.1  phospholipid-transporting ATPase IA isoform X5

    See identical proteins and their annotated locations for XP_006503766.1

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:11 → 922
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  2. XM_036164750.1 → XP_036020643.1  phospholipid-transporting ATPase IA isoform X4

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:49 → 1071
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  3. XM_011240698.4 → XP_011239000.1  phospholipid-transporting ATPase IA isoform X3

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:49 → 1096
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  4. XM_036164751.1 → XP_036020644.1  phospholipid-transporting ATPase IA isoform X6

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:1 → 907
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  5. XM_017320623.3 → XP_017176112.1  phospholipid-transporting ATPase IA isoform X1

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:62 → 1099
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  6. XM_017320624.3 → XP_017176113.1  phospholipid-transporting ATPase IA isoform X2

    UniProtKB/TrEMBL
    A1L332
    Conserved Domains (1) summary
    TIGR01652
    Location:62 → 1084
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

  7. XM_036164752.1 → XP_036020645.1  phospholipid-transporting ATPase IA isoform X7

    UniProtKB/TrEMBL
    Q52KQ7
    Conserved Domains (1) summary
    TIGR01652
    Location:1 → 707
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P70704.2 GenPept UniProtKB/Swiss-Prot:P70704

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