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DCTN3 dynactin subunit 3 [ Homo sapiens (human) ]

Gene ID: 11258, updated on 5-Mar-2024

Summary

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Official Symbol
DCTN3provided by HGNC
Official Full Name
dynactin subunit 3provided by HGNC
Primary source
HGNC:HGNC:2713
See related
Ensembl:ENSG00000137100 MIM:607387; AllianceGenome:HGNC:2713
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DCTN22; DCTN-22
Summary
This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Expression
Ubiquitous expression in brain (RPKM 30.6), bone marrow (RPKM 26.1) and 25 other tissues See more
Orthologs
mouse all
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Genomic context

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Location:
9p13.3
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 9 NC_000009.12 (34613546..34620495, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 9 NC_060933.1 (34632092..34639042, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (34613543..34620492, complement)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene ciliary neurotrophic factor receptor Neighboring gene uncharacterized LOC105376023 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:34570922-34571422 Neighboring gene CNTFR antisense RNA 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:34578559-34579533 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:34611012-34611565 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19850 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28311 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 28312 Neighboring gene ribonuclease P/MRP subunit p25 like Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:34624191-34624739 Neighboring gene AT-rich interaction domain 3C Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19851 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19852 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 19853 Neighboring gene sigma non-opioid intracellular receptor 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Assignment of p22 dynactin light chain (DCTN3) to human chromosome region 9p13 by radiation hybrid mapping. Mills DR, et al. Cytogenet Cell Genet, 2001. PMID 11306820
  2. Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells. Karki S, et al. J Cell Biol, 1998 Aug 24. PMID 9722614, Free PMC Article
  3. Study on the Prognostic Values of Dynactin Genes in Low-Grade Glioma. Su X, et al. Technol Cancer Res Treat, 2021 Jan-Dec. PMID 33896271, Free PMC Article
  4. Alteration of Motor Protein Expression Involved in Bidirectional Transport in Peripheral Blood Mononuclear Cells of Patients with Amyotrophic Lateral Sclerosis. Kuźma-Kozakiewicz M, et al. Neurodegener Dis, 2016. PMID 26954557
  5. A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1. Karki S, et al. J Biol Chem, 2000 Feb 18. PMID 10671518

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study on the Prognostic Values of Dynactin Genes in Low-Grade Glioma.
    Title: Study on the Prognostic Values of Dynactin Genes in Low-Grade Glioma.
  2. Study found that in peripheral blood mononuclear cells the median expression of KIFC3, KIF1B, and KIF5C was much lower than the expression of dynactin subunits DCTN1 and DCTN3, in both sporadic amyotrophic lateral sclerosis and healthy cases
    Title: Alteration of Motor Protein Expression Involved in Bidirectional Transport in Peripheral Blood Mononuclear Cells of Patients with Amyotrophic Lateral Sclerosis.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC111190

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables structural molecule activity TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in cytoskeleton-dependent cytokinesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cytoskeleton-dependent cytokinesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in microtubule-based process IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic cell cycle TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cleavage furrow IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
part_of dynactin complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of dynactin complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in kinetochore IEA
Inferred from Electronic Annotation
more info
  
located_in midbody IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
  
located_in perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
dynactin subunit 3
Names
dynactin 3 (p22)
dynactin complex subunit 22 kDa subunit
dynactin light chain

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001281425.2 → NP_001268354.1  dynactin subunit 3 isoform 3

    See identical proteins and their annotated locations for NP_001268354.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.
    Source sequence(s)
    BI837796, CN257020, DA212054
    Consensus CDS
    CCDS65028.1
    UniProtKB/TrEMBL
    X6RCK5
    Related
    ENSP00000368196.4, ENST00000378916.8
    Conserved Domains (1) summary
    pfam07426
    Location:10 → 142
    Dynactin_p22; Dynactin subunit p22

  2. NM_001281426.2 → NP_001268355.1  dynactin subunit 3 isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a translational frameshift, compared to variant 1. The encoded isoform (4) has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    BC107697, BI596981, CN257020, DA212054
    UniProtKB/TrEMBL
    X6RA56
    Related
    ENSP00000368193.2, ENST00000378913.6
    Conserved Domains (1) summary
    pfam07426
    Location:10 → 137
    Dynactin_p22; Dynactin subunit p22

  3. NM_001281427.2 → NP_001268356.1  dynactin subunit 3 isoform 5

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks an in-frame exon and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter and contains a distinct internal segment, compared to isoform 1.
    Source sequence(s)
    AW953496, CN257020, DA212054
    Consensus CDS
    CCDS65029.1
    UniProtKB/TrEMBL
    X6RCK5, X6RLR1
    Related
    ENSP00000432915.2, ENST00000477738.6
    Conserved Domains (1) summary
    pfam07426
    Location:10 → 160
    Dynactin_p22; Dynactin subunit p22

  4. NM_007234.5 → NP_009165.1  dynactin subunit 3 isoform 1

    See identical proteins and their annotated locations for NP_009165.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    BC107697, CN257020, DA212054
    Consensus CDS
    CCDS6560.1
    UniProtKB/Swiss-Prot
    A6NII7, B2RBM5, O75935, Q5T1I5, Q5T1I7, Q5T8G3, Q9BPU8
    UniProtKB/TrEMBL
    X6RCK5
    Related
    ENSP00000259632.7, ENST00000259632.12
    Conserved Domains (1) summary
    pfam07426
    Location:10 → 170
    Dynactin_p22; Dynactin subunit p22

  5. NM_024348.4 → NP_077324.1  dynactin subunit 3 isoform 2

    See identical proteins and their annotated locations for NP_077324.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a translational frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    BC000319, CN257020, DA212054
    Consensus CDS
    CCDS6561.1
    UniProtKB/Swiss-Prot
    O75935
    Related
    ENSP00000343986.2, ENST00000341694.6
    Conserved Domains (1) summary
    pfam07426
    Location:10 → 155
    Dynactin_p22; Dynactin subunit p22

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p14 Primary Assembly

    Range
    34613546..34620495 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060933.1 Alternate T2T-CHM13v2.0

    Range
    34632092..34639042 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O75935.1 GenPept UniProtKB/Swiss-Prot:O75935

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