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MSLN mesothelin [ Homo sapiens (human) ]

Gene ID: 10232, updated on 23-Mar-2024

Summary

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Official Symbol
MSLNprovided by HGNC
Official Full Name
mesothelinprovided by HGNC
Primary source
HGNC:HGNC:7371
See related
Ensembl:ENSG00000102854 MIM:601051; AllianceGenome:HGNC:7371
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MPF; SMRP
Summary
This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
Expression
Biased expression in lung (RPKM 16.4), stomach (RPKM 4.7) and 7 other tissues See more
Orthologs
mouse all
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Genomic context

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See MSLN in Genome Data Viewer
Location:
16p13.3
Exon count:
18
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 16 NC_000016.10 (760734..768865)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 16 NC_060940.1 (764710..773383)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 16 NC_000016.9 (810734..818865)

Chromosome 16 - NC_000016.10Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6949 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6950 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6951 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6952 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6953 Neighboring gene hydroxyacylglutathione hydrolase like Neighboring gene cytosolic iron-sulfur assembly component 3 Neighboring gene Sharpr-MPRA regulatory region 977 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:790544-791139 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6956 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr16:800614-801181 Neighboring gene MSLN promoter region Neighboring gene mesothelin like Neighboring gene microRNA 662 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:836847-837654 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6957 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6958 Neighboring gene RNA pseudouridine synthase domain containing 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Mesothelin is Commonly Expressed in Pancreatic Adenocarcinoma but Unrelated to Cancer Aggressiveness. Weidemann S, et al. Cancer Invest, 2021 Oct. PMID 34143695
  2. The exosomes derived from CAR-T cell efficiently target mesothelin and reduce triple-negative breast cancer growth. Yang P, et al. Cell Immunol, 2021 Feb. PMID 33373818
  3. Multiple proteases are involved in mesothelin shedding by cancer cells. Liu X, et al. Commun Biol, 2020 Dec 1. PMID 33262421, Free PMC Article
  4. Association of genetic variants in the 3'-untranslated region of the mesothelin (MSLN) gene with ovarian carcinoma. Abou-El-Naga AM, et al. J Biochem Mol Toxicol, 2021 Feb. PMID 32997381
  5. Possible reversibility between epithelioid and sarcomatoid types of mesothelioma is independent of ERC/mesothelin expression. Kojima M, et al. Respir Res, 2020 Jul 16. PMID 32677949, Free PMC Article

See all (171) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mesothelin expression in gynecologic carcinosarcoma: clinicopathological significance and correlation with HER2 expression.
    Title: Mesothelin expression in gynecologic carcinosarcoma: clinicopathological significance and correlation with HER2 expression.
  2. Enhanced Expression of Glycolytic Enzymes and Succinate Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute Myeloid Leukemia.
    Title: Enhanced Expression of Glycolytic Enzymes and Succinate Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute Myeloid Leukemia.
  3. Tumor resistance to anti-mesothelin CAR-T cells caused by binding to shed mesothelin is overcome by targeting a juxtamembrane epitope.
    Title: Tumor resistance to anti-mesothelin CAR-T cells caused by binding to shed mesothelin is overcome by targeting a juxtamembrane epitope.
  4. Novel mesothelin-targeted chimeric antigen receptor-modified UNKT cells are highly effective in inhibiting tumor progression.
    Title: Novel mesothelin-targeted chimeric antigen receptor-modified UNKT cells are highly effective in inhibiting tumor progression.
  5. High mesothelin expression by immunohistochemistry predicts improved survival in pleural mesothelioma.
    Title: High mesothelin expression by immunohistochemistry predicts improved survival in pleural mesothelioma.
  6. Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma.
    Title: Effects of hsa-mir-145-5p on the Regulation of msln Expression in Colorectal Adenocarcinoma.
  7. Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer.
    Title: Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer.
  8. Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.
    Title: Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.
  9. Host Mesothelin Expression Increases Ovarian Cancer Metastasis in the Peritoneal Microenvironment.
    Title: Host Mesothelin Expression Increases Ovarian Cancer Metastasis in the Peritoneal Microenvironment.
  10. Disruption of adenosine 2A receptor improves the anti-tumor function of anti-mesothelin CAR T cells both in vitro and in vivo.
    Title: Disruption of adenosine 2A receptor improves the anti-tumor function of anti-mesothelin CAR T cells both in vitro and in vivo.
Submit: New GeneRIF Correction See all GeneRIFs (149)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell adhesion NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in cell-matrix adhesion IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
is_active_in cell surface IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum lumen TAS
Traceable Author Statement
more info
  
located_in extracellular region TAS
Traceable Author Statement
more info
  
located_in membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in plasma membrane TAS
Traceable Author Statement
more info
  
located_in side of membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mesothelin
Names
CAK1 antigen
megakaryocyte potentiating factor
pre-pro-megakaryocyte-potentiating factor
soluble MPF mesothelin related protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001177355.3 → NP_001170826.1  mesothelin isoform 1 preproprotein

    See identical proteins and their annotated locations for NP_001170826.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).
    Source sequence(s)
    AL031258, AY743922, BC003512, BC009272
    Consensus CDS
    CCDS45370.1
    UniProtKB/Swiss-Prot
    Q13421
    Related
    ENSP00000456008.1, ENST00000563941.5
    Conserved Domains (1) summary
    pfam06060
    Location:1 → 608
    Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

  2. NM_005823.6 → NP_005814.2  mesothelin isoform 1 preproprotein

    See identical proteins and their annotated locations for NP_005814.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Both variants 1 and 3 encode the same isoform isoform (1).
    Source sequence(s)
    AL031258, AY743922, BC003512
    Consensus CDS
    CCDS45370.1
    UniProtKB/Swiss-Prot
    Q13421
    Related
    ENSP00000442965.2, ENST00000545450.7
    Conserved Domains (1) summary
    pfam06060
    Location:1 → 608
    Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

  3. NM_013404.4 → NP_037536.2  mesothelin isoform 2 preproprotein

    See identical proteins and their annotated locations for NP_037536.2

    Status: REVIEWED

    Description
    Transcript Variant: This transcript variant (2) uses an alternate splice site in the coding region, compared to variant 1. Variant 2 encodes isoform 2 which is longer than isoform 1.
    Source sequence(s)
    AL545401, BC003512, BC009272, CB266931, D49441
    Consensus CDS
    CCDS32356.1
    UniProtKB/Swiss-Prot
    D3DU65, Q13421, Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2, Q9UCB2, Q9UK57
    Related
    ENSP00000372313.3, ENST00000382862.7
    Conserved Domains (1) summary
    pfam06060
    Location:1 → 616
    Mesothelin; Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000016.10 Reference GRCh38.p14 Primary Assembly

    Range
    760734..768865
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060940.1 Alternate T2T-CHM13v2.0

    Range
    764710..773383
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q13421.2 GenPept UniProtKB/Swiss-Prot:Q13421

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