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CHAF1A chromatin assembly factor 1 subunit A [ Homo sapiens (human) ]

Gene ID: 10036, updated on 7-Apr-2024

Summary

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Official Symbol
CHAF1Aprovided by HGNC
Official Full Name
chromatin assembly factor 1 subunit Aprovided by HGNC
Primary source
HGNC:HGNC:1910
See related
Ensembl:ENSG00000167670 MIM:601246; AllianceGenome:HGNC:1910
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CAF1; P150; CAF-1; CAF1B; CAF1P150
Summary
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]
Expression
Broad expression in bone marrow (RPKM 7.4), testis (RPKM 6.1) and 24 other tissues See more
Orthologs
mouse all
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Genomic context

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Location:
19p13.3
Exon count:
18
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (4402640..4450830)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (4386080..4434290)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (4402637..4443394)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4350305-4350806 Neighboring gene eukaryotic translation initiation factor 1 pseudogene 6 Neighboring gene MPN domain containing Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4365919-4366840 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4368989-4369938 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4369939-4370886 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4372431-4373164 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4373165-4373898 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancers GRCh37_chr19:4374633-4375365 and GRCh37_chr19:4375366-4376099 Neighboring gene SH3 domain containing GRB2 like 1, endophilin A2 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4377567-4378299 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13771 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13772 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13773 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:4386482-4386982 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4389401-4390008 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4390009-4390615 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4390616-4391224 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4394991-4395501 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4399905-4400734 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4402397-4403094 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4403095-4403790 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4408505-4409316 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4409317-4410128 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:4412803-4413801 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:4430727-4431490 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4436676-4437176 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4437177-4437677 Neighboring gene Sharpr-MPRA regulatory region 9621 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:4444373-4444541 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4453327-4454026 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9890 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13774 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9891 Neighboring gene microRNA 4746 Neighboring gene UBX domain protein 6 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13775 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13776 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9892 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 9893 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4474209-4475158 Neighboring gene HDGF like 2 Neighboring gene perilipin 4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr19:4534387-4534942 Neighboring gene perilipin 5

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Unorthodox PCNA Binding by Chromatin Assembly Factor 1. Gopinathan Nair A, et al. Int J Mol Sci, 2022 Sep 21. PMID 36232396, Free PMC Article
  2. Detection of CAF-1/p60 in peripheral blood as a potential biomarker of HNSCC tumors. Merolla F, et al. Oral Oncol, 2021 Sep. PMID 34237585
  3. CHAF1A overexpression in human retinoblastoma promotes cell proliferation and suppresses apoptosis. Shen J, et al. J BUON, 2020 Sep-Oct. PMID 33277876
  4. CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression. Zheng L, et al. EBioMedicine, 2018 Dec. PMID 30449701, Free PMC Article
  5. Emerging roles of the histone chaperone CAF-1 in cellular plasticity. Cheloufi S, et al. Curr Opin Genet Dev, 2017 Oct. PMID 28692904, Free PMC Article

See all (137) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
    Title: Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
  2. Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor autophagy that endows a therapeutical vulnerability in diffuse large B cell lymphoma.
    Title: Aberrant SPOP-CHAF1A ubiquitination axis triggers tumor autophagy that endows a therapeutical vulnerability in diffuse large B cell lymphoma.
  3. CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
    Title: CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection.
  4. CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
    Title: CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
  5. Detection of CAF-1/p60 in peripheral blood as a potential biomarker of HNSCC tumors.
    Title: Detection of CAF-1/p60 in peripheral blood as a potential biomarker of HNSCC tumors.
  6. DNA methylation mediated downregulation of histone H3 variant H3.3 affects cell proliferation contributing to the development of HCC.
    Title: DNA methylation mediated downregulation of histone H3 variant H3.3 affects cell proliferation contributing to the development of HCC.
  7. Identification and external validation of a prognostic signature associated with DNA repair genes in gastric cancer.
    Title: Identification and external validation of a prognostic signature associated with DNA repair genes in gastric cancer.
  8. CHAF1A overexpression in human retinoblastoma promotes cell proliferation and suppresses apoptosis.
    Title: CHAF1A overexpression in human retinoblastoma promotes cell proliferation and suppresses apoptosis.
  9. Histone Loaders CAF1 and HIRA Restrict Epstein-Barr Virus B-Cell Lytic Reactivation.
    Title: Histone Loaders CAF1 and HIRA Restrict Epstein-Barr Virus B-Cell Lytic Reactivation.
  10. Role of chromatin assembly factor-1/p60 and poly [ADP-ribose] polymerase 1 in mycosis fungoides.
    Title: Role of chromatin assembly factor-1/p60 and poly [ADP-ribose] polymerase 1 in mycosis fungoides.
Submit: New GeneRIF Correction See all GeneRIFs (35)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of chromatin assembly factor 1, subunit A (CHAF1A) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 is identified to have a physical interaction with chromatin assembly factor 1, subunit A (CHAF1A; CAF1B) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with chromatin assembly factor 1, subunit A (CHAF1A; CAF1B) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC71229

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables chromatin binding TAS
Traceable Author Statement
more info
 PubMed 
enables chromo shadow domain binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables unfolded protein binding TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication-dependent chromatin assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleosome assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleosome assembly IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of CAF-1 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of CAF-1 complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of CAF-1 complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of chromatin IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
chromatin assembly factor 1 subunit A
Names
CAF-1 subunit A
CAF-I 150 kDa subunit
CAF-I p150
CTB-50L17.7
chromatin assembly factor I (150 kDa)
chromatin assembly factor I p150 subunit
hp150

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_005483.3 → NP_005474.2  chromatin assembly factor 1 subunit A

    See identical proteins and their annotated locations for NP_005474.2

    Status: VALIDATED

    Source sequence(s)
    AA576757, AC011498, BC052620, BE796132, BG775588, BM826511, BQ889365, H98924, U20979
    Consensus CDS
    CCDS32875.1
    UniProtKB/Swiss-Prot
    Q13111, Q6NXG5, Q7Z7K3, Q9UJY8
    Related
    ENSP00000301280.4, ENST00000301280.10
    Conserved Domains (4) summary
    pfam11600
    Location:320 → 479
    CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
    pfam12253
    Location:558 → 601
    CAF1A; Chromatin assembly factor 1 subunit A
    pfam15539
    Location:665 → 954
    CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
    pfam15557
    Location:1 → 226
    CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    4402640..4450830
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011527605.3 → XP_011525907.1  chromatin assembly factor 1 subunit A isoform X1

    Conserved Domains (4) summary
    pfam11600
    Location:320 → 479
    CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
    pfam12253
    Location:558 → 601
    CAF1A; Chromatin assembly factor 1 subunit A
    pfam15539
    Location:665 → 943
    CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
    pfam15557
    Location:1 → 226
    CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

  2. XM_011527607.3 → XP_011525909.1  chromatin assembly factor 1 subunit A isoform X2

    Conserved Domains (4) summary
    pfam11600
    Location:382 → 460
    CAF-1_p150; Chromatin assembly factor 1 complex p150 subunit, N-terminal
    pfam12253
    Location:539 → 582
    CAF1A; Chromatin assembly factor 1 subunit A
    pfam15539
    Location:646 → 935
    CAF1-p150_C2; CAF1 complex subunit p150, region binding to CAF1-p60 at C-term
    pfam15557
    Location:1 → 226
    CAF1-p150_N; CAF1 complex subunit p150, region binding to PCNA

  3. XM_047438011.1 → XP_047293967.1  chromatin assembly factor 1 subunit A isoform X3

  4. XM_047438012.1 → XP_047293968.1  chromatin assembly factor 1 subunit A isoform X3

  5. XM_047438013.1 → XP_047293969.1  chromatin assembly factor 1 subunit A isoform X3

RNA

  1. XR_007066505.1 RNA Sequence

  2. XR_936135.3 RNA Sequence

  3. XR_007066507.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    4386080..4434290
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054319464.1 → XP_054175439.1  chromatin assembly factor 1 subunit A isoform X1

  2. XM_054319465.1 → XP_054175440.1  chromatin assembly factor 1 subunit A isoform X4

  3. XM_054319467.1 → XP_054175442.1  chromatin assembly factor 1 subunit A isoform X5

  4. XM_054319466.1 → XP_054175441.1  chromatin assembly factor 1 subunit A isoform X2

  5. XM_054319468.1 → XP_054175443.1  chromatin assembly factor 1 subunit A isoform X3

  6. XM_054319469.1 → XP_054175444.1  chromatin assembly factor 1 subunit A isoform X3

RNA

  1. XR_008485038.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q13111.3 GenPept UniProtKB/Swiss-Prot:Q13111

Gene LinkOut

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