CYP2B4 cytochrome P450, family 2, subfamily b, polypeptide 4 [Oryctolagus cuniculus (rabbit)] - Gene

Summary

Gene symbol: CYP2B4
Gene description: cytochrome P450, family 2, subfamily b, polypeptide 4
See related: Ensembl:ENSOCUG00000004655
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Oryctolagus cuniculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus
Also known as: CYP2B5; CYPIIB4; CYPIIB5; P450-LM2; P-450 2B5
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Genomic context

Location:: chromosome: Un

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2023_02	current	UM_NZW_1.0 (GCF_009806435.1)	Unplaced Scaffold	NW_026259230.1 (735599..747415, complement)

NW_026259230.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NW_026259230 Unplaced Scaffold Reference UM_NZW_1.0 Primary Assembly

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate not only that binding of b5 and P450 reductase on the proximal surface of P450 2B4 results in electron transfer but also that each redox partner transmits unique structural information to the active site proton delivery network.
Title: Protonation of the Hydroperoxo Intermediate of Cytochrome P450 2B4 Is Slower in the Presence of Cytochrome P450 Reductase Than in the Presence of Cytochrome b5.
the observation of weak and nonspecific interactions between CYP2B4 and cytb5 in micelles suggests that lipid bilayer structures and low curvature membrane surface are preferable for CYP2B4-cytb5 complex formation.
Title: Effects of membrane mimetics on cytochrome P450-cytochrome b5 interactions characterized by NMR spectroscopy.
Removal of cholesterol (an important constituent of ordered domains) led to the relocation of CYP1A2, CYP2B4 and CPR to the disordered regions.
Title: Cytochrome P450 system proteins reside in different regions of the endoplasmic reticulum.
Mutations R73 in P450 2B6 and R73, V216, L219, and F220 in 2B4 are important for catalytic efficiency.
Title: The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes.
Data suggest that the cytb5-cytP4502B4 complex structure will help unravel the mechanism by which cytb5 regulates catalysis by cytP4502B4.
Title: A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data.
Oxidation kinetics of toluene in CYP1A2-CYP2B4 mixtures with and without cytochrome p450 reductase (CPR) are consistent with a model in which CYP1A2 has a higher affinity for CPR binding.
Title: Interactions between cytochromes P450 2B4 (CYP2B4) and 1A2 (CYP1A2) lead to alterations in toluene disposition and P450 uncoupling.
Data suggest rearrangements of secondary structural elements and side chains of CYP2B4 required for ligand binding; studies elucidate structure of CYP2B4 in complex with paroxetine (second-generation antidepressant and CYP2B4 inhibitor).
Title: A structural snapshot of CYP2B4 in complex with paroxetine provides insights into ligand binding and clusters of conformational states.
Two different types of cytochrome b5 orientations are present within a MFO system: the first allowing potential cytochrome b5 electron donation to P450, the second one inducing cytochrome b5 modulation of P450 structural changes.
Title: Mapping of interaction between cytochrome P450 2B4 and cytochrome b5: the first evidence of two mutual orientations.
investigation of metabolism of ticlopidine by CYP2B4: identification of metabolites formed by recombinant CYP2B4
Title: Comparison of in vitro metabolism of ticlopidine by human cytochrome P450 2B6 and rabbit cytochrome P450 2B4.
a structural model of a drug and its metabolites complexed to a cytochrome P450 based on NMR
Title: NMR-derived models of amidopyrine and its metabolites in complexes with rabbit cytochrome P450 2B4 reveal a structural mechanism of sequential N-dealkylation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RefSeq

Function	Evidence Code	Pubs
enables heme binding	IEA Inferred from Electronic Annotation more info	PubMed
enables iron ion binding	IEA Inferred from Electronic Annotation more info	PubMed
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen	IEA Inferred from Electronic Annotation more info	PubMed

Process	Evidence Code	Pubs
involved_in organic acid metabolic process	IEA Inferred from Electronic Annotation more info	PubMed
involved_in xenobiotic metabolic process	IEA Inferred from Electronic Annotation more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info	PubMed
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info	PubMed

General protein information

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Preferred Names: cytochrome P450 2B4

Names: Cytochrome P450 2B5; Cytochrome P450 form HP1; Cytochrome P450 type B2; P450 types B0 and B1; cytochrome P-450 2B5; cytochrome P450 LM2; cytochrome P450 isozyme 2; cytochrome P450 type B0; cytochrome P450 type B1; isozyme 2

NP_001164330.2

EC 1.14.14.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.