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MED6 mediator complex subunit 6 [ Homo sapiens (human) ]

Gene ID: 10001, updated on 5-Mar-2024

Summary

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Official Symbol
MED6provided by HGNC
Official Full Name
mediator complex subunit 6provided by HGNC
Primary source
HGNC:HGNC:19970
See related
Ensembl:ENSG00000133997 MIM:602984; AllianceGenome:HGNC:19970
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
ARC33; NY-REN-28
Summary
Enables transcription coactivator activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of mediator complex. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in lymph node (RPKM 6.4), ovary (RPKM 5.9) and 25 other tissues See more
Orthologs
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Genomic context

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See MED6 in Genome Data Viewer
Location:
14q24.2
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 14 NC_000014.9 (70583221..70600655, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 14 NC_060938.1 (64789242..64806677, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 14 NC_000014.8 (71049938..71067372, complement)

Chromosome 14 - NC_000014.9Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105370556 Neighboring gene ADAM metallopeptidase domain 20 Neighboring gene ADAM metallopeptidase domain 20 pseudogene Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr14:71066874-71068073 Neighboring gene double homeobox A pseudogene 12 Neighboring gene keratin 18 pseudogene 7 Neighboring gene RNA, 7SL, cytoplasmic 77, pseudogene Neighboring gene TTC9 divergent transcript Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8655 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr14:71102515-71103714 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8656 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8657 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5887 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5888 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5889 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr14:71120238-71120824 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5890 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8658 Neighboring gene tetratricopeptide repeat domain 9 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8659 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:71133100-71133600 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:71133601-71134101

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription. Sun X, et al. Mol Cell, 1998 Aug. PMID 9734358
  2. The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator. Yang F, et al. Proc Natl Acad Sci U S A, 2004 Feb 24. PMID 14983011, Free PMC Article
  3. Characterization of mediator complexes from HeLa cell nuclear extract. Wang G, et al. Mol Cell Biol, 2001 Jul. PMID 11416138, Free PMC Article
  4. Mediator CDK subunits are platforms for interactions with various chromatin regulatory complexes. Fukasawa R, et al. J Biochem, 2012 Sep. PMID 22668559
  5. Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation. Ding N, et al. Mol Cell, 2008 Aug 8. PMID 18691967, Free PMC Article

See all (80) citations in PubMed

GeneRIFs: Gene References Into Functions

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed
Knockdown of mediator complex subunit 6 (MED6) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat Knockdown of MED6 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed
tat The interaction of HIV-1 Tat with MED21 hypothetically induces the binding of Tat to MED6 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coactivator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transcription coactivator binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in somatic stem cell population maintenance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of core mediator complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mediator complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in membrane HDA PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ubiquitin ligase complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 6
Names
CTD-2540L5.5
activator-recruited cofactor 33 kDa component
renal carcinoma antigen NY-REN-28

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001284209.2 → NP_001271138.1  mediator of RNA polymerase II transcription subunit 6 isoform 1

    See identical proteins and their annotated locations for NP_001271138.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.
    Source sequence(s)
    AK300460, BM999935, DA869309
    Consensus CDS
    CCDS61484.1
    UniProtKB/TrEMBL
    A8K3N2
    Related
    ENSP00000413343.2, ENST00000430055.6
    Conserved Domains (1) summary
    pfam04934
    Location:13 → 146
    Med6; MED6 mediator sub complex component

  2. NM_001284210.2 → NP_001271139.1  mediator of RNA polymerase II transcription subunit 6 isoform 3

    See identical proteins and their annotated locations for NP_001271139.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site and lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AK300460, AK304361, BM999935, DA869309
    Consensus CDS
    CCDS61483.1
    UniProtKB/Swiss-Prot
    O75586
    Related
    ENSP00000394502.2, ENST00000440435.2
    Conserved Domains (1) summary
    pfam04934
    Location:13 → 139
    Med6; MED6 mediator sub complex component

  3. NM_001284211.2 → NP_001271140.1  mediator of RNA polymerase II transcription subunit 6 isoform 4

    See identical proteins and their annotated locations for NP_001271140.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) uses an alternate splice site and differs in the 3' exon structure, which results in a frameshift, compared to variant 1. The encoded isoform (4) is longer and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AK300460, BM802752, BM999935, DA869309, HY064772
    Consensus CDS
    CCDS73649.1
    UniProtKB/TrEMBL
    A0A087WYL7, A8K3N2
    Related
    ENSP00000481920.1, ENST00000615788.4
    Conserved Domains (1) summary
    pfam04934
    Location:13 → 139
    Med6; MED6 mediator sub complex component

  4. NM_005466.4 → NP_005457.2  mediator of RNA polymerase II transcription subunit 6 isoform 2

    See identical proteins and their annotated locations for NP_005457.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AL357153, BC004106, BM999935, DA869309, HY064772
    Consensus CDS
    CCDS9805.1
    UniProtKB/Swiss-Prot
    B4DU17, B4E2P0, O15401, O75586, Q53FE3, Q53HJ3, Q6FHQ4, Q9BTH1, Q9UHL1
    UniProtKB/TrEMBL
    A8K3N2
    Related
    ENSP00000256379.5, ENST00000256379.10
    Conserved Domains (1) summary
    pfam04934
    Location:13 → 140
    Med6; MED6 mediator sub complex component

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000014.9 Reference GRCh38.p14 Primary Assembly

    Range
    70583221..70600655 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060938.1 Alternate T2T-CHM13v2.0

    Range
    64789242..64806677 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O75586.2 GenPept UniProtKB/Swiss-Prot:O75586

Gene LinkOut

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