GEO DataSets

(Submitter supplied) The antifungal compound fludioxonil targets the fungal specific group III hybrid histidin kinaseTcsC of A. fumigatus, which in turn activate the High Osmolarity Glycerol (HOG) pathway and leads to drastic swelling of the fungal cells. We investigated the different responses of the AfS35 (wild type), the completely resistant mutant strain DtcsC and the partial resistant mutant strain Dskn7 to fludioxonil.

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

36 Samples

Download data: XLS

(Submitter supplied) The transcription factor CrzA influences cell wall organization in the pathogenic fungus Aspergillus fumigatus, and also binds to the promoter regions of chitin synthase genes upon exposure to the antifungal drug caspofungin. To gain an overview of the genes directly regulated by CrzA, the CrzA binding sites were determined genome-wide by ChIP-seq

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

4 Samples

Download data: BW

(Submitter supplied) Microarray analysis was used to identify the osmotic stress-responsive genes dependent on HogA and AtfA in the filamentous fungus Aspergillus nidulans. In order to identify such genes, we conducted the several types of experiment. One was a comparison between wild type with and without osmotic shock (Exp.6). Others were comparison between wild type with osmotic shock and each mutant (hogA, Exp.7; atfA, Exp.8) with osmotic shock. more...

Organism:

Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL7364

6 Samples

Download data: GPR

(Submitter supplied) Microarray analysis was used to identify the fludioxonil-responsive genes dependent on SskA, SrrA, HogA, and AtfA in the filamentous fungus Aspergillus nidulans. In order to identify such genes, we conducted the several types of experiment. One was a comparison between wild type treated with fludioxonil and without the treatment (Exp.1). Others were comparison between wild type treated with fludioxonil and each mutant (sskA, Exp.2; srrA, Exp.3; hogA, Exp.4; atfA, Exp.5) treated with fludioxonil. more...

Organism:

Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL7364

10 Samples

Download data: GPR

(Submitter supplied) Comparison of Candida albicans SC5314 treated with fludioxonil for 30 min and untreated controls under hyphae-inducing conditions

Organism:

Candida albicans SC5314; Candida albicans

Type:

Expression profiling by array

Platform:

GPL15859

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Aspergillus fumigatus Af293

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL25869 GPL25907

14 Samples

Download data: TXT, WIG

(Submitter supplied) Determine the transcriptomic response of Aspergillus fumigatus strains to different alleles of atrR

Organism:

Aspergillus fumigatus Af293

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25907

8 Samples

Download data: TXT, WIG

(Submitter supplied) To determine the genomic binding sites for the Aspergillus fumigatus transcription factor AtrR, we utilized three different strains. One was the wild-type AfS35 which contains a wild-type copy of atrR but lacks any HA tag. The other two are both derived from AfS35 but contain a 3X HA tag replacing the stop codon of atrR. These strains are SPF89 and SPF112, respectively. SPF89 uses the wild-type atrR promoter to drive expression of the epitope-tagged allele while SPF112 uses the much strong hspA promoter to drive production of the same protein.

Organism:

Aspergillus fumigatus Af293

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25869

6 Samples

Download data: WIG

(Submitter supplied) Aspergillus fumigatus is a human-pathogenic mold that extracts nutrients from the environment or from host tissues by secreting hydrolytic enzymes. The ability of A. fumigatus to adjust secretion levels in proportion to demand relies on the assistance of the unfolded protein response (UPR), an adaptive stress response pathway that regulates the unique protein-folding environment of the endoplasmic reticulum (ER). more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30331

6 Samples

Download data: XLSX

(Submitter supplied) We use ChIP-seq technique to access RNA polymerase II enrichment in wild type and nsdC A. fumigatus null mutant before and after calcium exposure

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

8 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

26 Samples

Download data: BIGWIG

(Submitter supplied) The Negative cofactor 2 (NCT) complex is an evolutionally conserved heterodimeric transcription factor. In Aspergillus fumigatus, the NCT complex consists of two subunits NctA and NctB. Through a genome-wide screening of a transcription factor null mutant strains, we found that loss of the NCT complex leads to a multi-drug resistance phenotype including the azoles (itraconazole, voriconazole and posaconazole) as well as the salvage therapeutic amphotericin B, and terbinafine. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23160

18 Samples

Download data: TXT

(Submitter supplied) The Negative cofactor 2 (NCT) complex is an evolutionally conserved heterodimeric transcription factor. In Aspergillus fumigatus, the NCT complex consists of two subunits NctA and NctB. Through a genome-wide screening of a transcription factor null mutant strains, we found that loss of the NCT complex leads to a multi-drug resistance phenotype including the azoles (itraconazole, voriconazole and posaconazole) as well as the salvage therapeutic amphotericin B, and terbinafine. more...

Organism:

Aspergillus fumigatus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23160

8 Samples

Download data: BIGWIG

(Submitter supplied) Amphotericin B (AMB) is the most widely used polyene antifungal drug for the treatment of systemic fungal infections including invasive aspergillosis. We aimed to understand molecular targets of AMB in Aspergillus fumigatus (Afu) by genomic approaches. Keywords: Aspergillus fumigatus treated with amphotericin B for 24 hours

Organism:

Aspergillus fumigatus

Type:

Expression profiling by array

Platform:

GPL6430

2 Samples

Download data: TXT

(Submitter supplied) The genome of the osmophilic Aspergillus wentii, unlike that of the osmotolerant Aspergillus nidulans, contains only the gfdA but not the gfdB glycerol 3-phosphate dehydrogenase gene. Here, we studied transcriptomic changes of A. nidulans (reference strain and DgfdB gene deletion mutant) and A. wentii (reference strain and An-gfdB expressing mutant) elicited by high osmolarity. A. nidulans showed canonic hyperosmotic stress response characterized by upregulation of trehalose and glycerol metabolism genes (including gfdB) as well as genes of the high-osmolarity glycerol (HOG) map kinase pathway. more...

Organism:

Aspergillus nidulans; Aspergillus wentii

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL34198 GPL28598

48 Samples

Download data: XLSX

(Submitter supplied) Pathogens have to cope with oxidative, iron- and carbon-limitation stresses in the human body. To understand how combined iron-carbon limitation alters oxidative stress response, Aspergillus fumigatus was cultured in glucose-peptone or peptone containing media supplemented or not with deferiprone as iron chelator. Changes in the transcriptome in these cultures were recorded after H2O2 treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. more...

Organism:

Aspergillus fumigatus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23268

24 Samples

Download data: XLSX