GEO DataSets

(Submitter supplied) Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) Proteins produced in the salivary glands of arthropod herbivores can function as effectors to modify plant defense responses. To obtain a set of candidate, salivary gland specific genes for the mite herbivore Tetranychus urticae, mites were dissected and the head region was recovered. Specifically, the proterosoma was dissected from 250 mites. This includes the salivary glands, and other nearby tissue (given their small size, exact micro-dissection of salivary glands was not possible). more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21813

4 Samples

Download data: TXT

(Submitter supplied) A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

11 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14176

26 Samples

Download data: TXT

(Submitter supplied) We generated 38-bp Illumina reads from messenger RNA libraries from mites transferred from their preferred laboratory host, bean (Phaseolus vulgaris cv. California Red Kidney), to one of three hosts: bean, Arabidopsis thaliana (Bla-2 accession) and the tomato (Solanum lycopersicum; genotype Heinz 1706). Larvae were carefully collected from bean plants and transferred to the treatment plant. Mites were reared on these plants for ~24 hours, after which mites were collected for mRNA library preparation. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

9 Samples

Download data: TXT

(Submitter supplied) We generated 38-bp Illumina reads from single messenger RNA libraries from three diverse developmental stages of the two-spotted spider mite to capture small RNA diversity across development. Adult, nymphal+larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for small RNA library preparation. Samples were a mix of males and females to capture male and female patterns of small RNA composition and were reared on beans (Phaseolus vulgaris cv California Red Kidney). more...

Organism:

Tetranychus urticae

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14176

3 Samples

Download data: TXT

(Submitter supplied) We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

8 Samples

Download data: TXT

(Submitter supplied) We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14176

6 Samples

Download data: TXT

(Submitter supplied) We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes.

Organism:

Drosophila montana

Type:

Expression profiling by array

Platform:

GPL9293

9 Samples

Download data: TXT

(Submitter supplied) In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt. By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation. Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

12 Samples

Download data: TXT

(Submitter supplied) 250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) The goal of our microarray experiments was twofold: 1) Compare the gene expression profile of acaricide resistant spider mite strains (MAR-AB and MR-VP) with that of a susceptible spider mite strain (London); 2) Study gene expression changes in spider mites from the London strain upon transfer from bean, a suitable host, to tomato, a less favorable host. These gene expression changes upon host change were measured for three timepoints (2 hour on tomato (Tomato-2h), 12 hour on tomato (Tomato-12h) and 5 generations on tomato (Tomato-5G)).

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL15756

23 Samples

Download data: TXT

(Submitter supplied) While pathogen-induced immunity is comparatively well characterized, far less is known about plant defense responses to arthropod herbivores. To date, most molecular-genetic studies of plant-arthropod interactions have focused on insects. However, plant-feeding (phytophagous) mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g., Lepidopteran larvae or aphids). more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL16112

28 Samples

Download data: CEL

(Submitter supplied) Our goal was to investigate the transcriptional response of T. urticae to different Arabidopsis plants with varying levels of indole glucosinolates.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

12 Samples

Download data: TXT

(Submitter supplied) Most northern insect species experience a period of developmental arrest, diapause, which enables them to survive over the winter and postpone reproduction until favorable conditions. We studied the timing of reproductive diapause and its long-term effects on the cold tolerance of Drosophila montana, D. littoralis and D. ezoana females in seasonally varying environmental conditions. At the same time we traced expression levels of 219 genes in D. more...

Organism:

Drosophila virilis; Drosophila montana

Type:

Expression profiling by array

Platform:

GPL14008

23 Samples

Download data: TXT

(Submitter supplied) Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genome-wide transcriptional changes in both spider mite and tomato as a consequence of mite’s adaptation to tomato We used microarray to assess global gene expression in Solanum lycopersicum cv. more...

Organism:

Solanum lycopersicum

Type:

Expression profiling by array

Platform:

GPL16358

20 Samples

Download data: CEL

(Submitter supplied) Genome annotation of the chelicerate Tetranychus urticae revealed the absence of many canonical immunity genes. T. urticae either does not mount an immune response or it induces uncharacterized immune pathways. To disentangle these two hypotheses, we performed transcriptomic analysis of mites injected with bacteria vs mites injected with LB-buffer. Two types of bacteria were injected: E. coli and B. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

26 Samples

Download data: TXT

(Submitter supplied) Here, we examined the transcriptomic changes associated with diapause and CO2 treatment in B. terrestris queens before, during and post diapause and in the short and long term after CO2 treatment

Organism:

Bombus terrestris

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19585 GPL20914

25 Samples

Download data: GFF, TXT

(Submitter supplied) Here, we present the results of an integrative study that leverages naturally segregating variation and a recent, adaptive divergence event affecting seasonal timing to identify developmental mechanisms underlying diapause progression in a tephritid fly, Rhagoletis pomonella. Also called the apple maggot fly, R. pomonella is native to North America, where it infests fruits of native Crataegus (hawthorn) species throughout its range. more...

Organism:

Rhagoletis pomonella

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27760

50 Samples

Download data: TXT