GEO DataSets

(Submitter supplied) The amino acid biosynthetic pathway of invasive pathogenic fungi has been studied as a potential antifungal drug target. Studies of the disruption of genes involved in amino acid biosynthesis have demonstrated the importance of this pathway in the virulence of Cryptococcus neoformans. Here, we identified the MET5 (CNL05500) and MET10 (CNG03990) genes in this pathway, both encoding sulfite reductase, which catalyzes the reduction of sulfite to sulfide. more...

Organism:

Cryptococcus neoformans var. neoformans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28805

4 Samples

Download data: TXT

(Submitter supplied) The goal of this study was to position all transcripts extremities in two species of Cryptococcus using TSS-Seq and QuantSeq 3' mRNA-Seq when cells are grown under different conditions. We analysed also the level of expression of each genes in the same condition using the same cell sample. All these data have spiked in using a fixed quantity of S. cereviae cells added just before DNA and RNA extraction.

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL26812 GPL19081

108 Samples

Download data: TXT, WIG

(Submitter supplied) We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL26812 GPL19081

12 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Mating pheromone is the best-known fungal paracrine signal whose essentiality in determining sexual reproduction has been recognized since six decades ago. We demonstrate that the pheromone cannot induce mating response during unisexual mating due to the lack of a compatible receptor from the same unisexual population. We identify a quorum sensing (QS) peptide Qsp1 in place of pheromone as a specific intercellular signal directing Cryptococcus unisexual reproduction. more...

Organism:

Cryptococcus neoformans var. neoformans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22990

64 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans

Type:

Expression profiling by array

Platforms:

GPL4009 GPL15151

20 Samples

Download data: CSV, GPR

(Submitter supplied) Changes in gene expression of the serotype A pre-passage wild-type strain H99W with two mouse-passaged strains were examined during log-phase growth at 37 ºC.

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans

Type:

Expression profiling by array

Platform:

GPL4009

8 Samples

Download data: CSV, TXT

(Submitter supplied) To identify the genome-wide transcriptional changes that occur throughout germination of C. neoformans spores, we conducted a time-course microarray experiment spanning six timepoints to generate a temporal expression pattern for each known gene. Spores were placed in rich medium and allowed to germinate for 10 hours, when they start to replicate as yeast. Each time point was flash frozen in liquid nitrogen and RNA was harvested for each time point at the same time. more...

Organism:

Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

6 Samples

Download data: GPR

(Submitter supplied) To identify genes regulated by the homeodomain transcription factors Sxi1α and Sxi2a (Sex Inducer 1α and 2a) in Cryptococcus neoformans, we carried out a whole-genome expression experiment. We performed two independent whole-genome microarray experiments comparing transcript levels in cells that either possessed or were lacking the Sxi proteins. In the first experiment we compared the expression profile of a haploid sxi1αΔ strain to that of a haploid strain expressing both SXI1α and an inducible copy of SXI2a (Sxi +). more...

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL18646

2 Samples

Download data: GPR, TXT

(Submitter supplied) To identify the genome-wide transcriptional changes that occur throughout sexual developmen of C. neoformans, we conducted a time-course microarray experiment spanning six developmental stages to generate a temporal expression pattern for each known gene. C. neoformans a x α co-cultures were grown under sexual development conditions (V8 agar), and RNA was harvested at 0.5, 6, 12, 24, 48, and 72 hours post-mixing. more...

Organism:

Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

5 Samples

Download data: GPR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans; Cryptococcus neoformans var. grubii H99

Type:

Expression profiling by array

Platforms:

GPL10872 GPL4009

26 Samples

Download data

(Submitter supplied) Ubiquitination is a reversible protein modification involved in various cellular processes in eukaryotic cells. Deubiquitinating enzymes, the proteins responsible for the removal of ubiquitin, act as essential regulators to maintain ubiquitin homeostasis and to exquisitely regulate protein degradation via the ubiquitination pathway. Cryptococcus neoformans is an important basidiomycete pathogen that causes life-threatening meningoencephalitis primarily within the immunocompromised population. more...

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans

Type:

Expression profiling by array

Platform:

GPL4009

6 Samples

Download data: GPR, TXT

(Submitter supplied) Ubiquitination is a reversible protein modification involved in various cellular processes in eukaryotic cells. Deubiquitinating enzymes, the proteins responsible for the removal of ubiquitin, act as essential regulators to maintain ubiquitin homeostasis and to exquisitely regulate protein degradation via the ubiquitination pathway. Cryptococcus neoformans is an important basidiomycete pathogen that causes life-threatening meningoencephalitis primarily within the immunocompromised population. more...

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

4 Samples

Download data: GPR, TXT

(Submitter supplied) Changes in gene expression of serotype D strains JEC21 or 24067 (52D) were examined after incubation with mAb 18B7 or the control MOPC-21 for 1, 2 or 4 hours at 37 C.

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platforms:

GPL15151 GPL4009

28 Samples

Download data: CSV, GPR

(Submitter supplied) To understand the relationship between gene expression and capsule formation, H99 cells were cultured overnight at 37ºC in the following eight conditions: low iron medium with or without both 500 mM ethylenediaminetetraacetic acid (EDTA) and 10 mM bathophenanthroline disulfonate (BPDS); phosphate-buffered saline (PBS) with or without 10% v/v fetal bovine serum; Dulbecco's Modified Eagle's Medium (from Sigma) in ambient air or 5% CO2; and Littman's medium with either 0.01 µg/ml or 1 µg/ml thiamine.

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

22 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL14564 GPL14571 GPL4009

54 Samples

Download data: BED, CSV, CUFF, SAM, WIG

(Submitter supplied) Analysis of ccr4 Delta vs. wild type shifted from 30oC to 37oC for 10 minutes To determine the effect of ccr4 deletion on temperature stress-responsive changes in gene expression in C. neoformans

Organism:

Cryptococcus neoformans; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

1 Sample

Download data: TXT

(Submitter supplied) Cryptococcus neoformans is a prevalent human fungal pathogen that must survive within various tissues in order to establish a human infection. We have identified the C. neoformans Rim101 transcription factor, a highly conserved pH-response regulator in many fungal species. The rim101- mutant strain displays growth defects similar to other fungal species in the presence of alkaline pH, increased salt concentrations, and iron limitation. more...

Organism:

Cryptococcus neoformans var. grubii; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

1 Sample

Download data: GPR

(Submitter supplied) Light is a universal environmental signal perceived by many organisms, including the fungi in which light regulates both common and unique biological processes depending on the species. We conducted a whole-genome microarray analysis on the basidiomycete fungus Cryptococcus neoformans to identify light-regulated genes.

Organism:

Cryptococcus neoformans var. neoformans; Cryptococcus neoformans var. neoformans JEC21

Type:

Expression profiling by array

Platform:

GPL8638

6 Samples

Download data: GPR

(Submitter supplied) The Cryptococcus neoformans NRG1 gene was identified using gene microarrays to define putative transcription factor genes regulated by the cyclic AMP (cAMP) signal transduction pathway. Disruption of NRG1 results in delayed capsule formation and mating, two phenotypes that are directly controlled by cAMP signaling. Putative targets of the Nrg1 transcription factor were identified using a second genome microarray to define differences in the transcriptomes of the wild-type and nrg1 mutant strains. more...

Organism:

Cryptococcus neoformans var. grubii H99; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

1 Sample

Download data: GPR

(Submitter supplied) Rho-GDP dissociation inhibitors (RDI) are repressors of Rho-type monomeric GTPases that allow for precise control of their target processes, e.g. cytoskeletal arrangement, vesicle trafficking, and polarized growth. In the human pathogenic yeast Cryptococcus neoformans, maintenance of normal cell morphology is vital for pathogenicity. We identified and deleted the gene encoding an RDI homolog in the human fungal pathogen Cryptococcus neoformans and investigated its impact on pathogenicity in animal models of cryptococcosis. more...

Organism:

Cryptococcus neoformans var. grubii H99; Cryptococcus neoformans var. neoformans

Type:

Expression profiling by array

Platform:

GPL4009

1 Sample

Download data: GPR