GEO DataSets

(Submitter supplied) This study used yak and cattle-yak testes from different developmental stages as materials to construct a complete translation map of the testes, and integrated transcriptome and translation results to explore gene expression changes during the sexual maturation process of yak testes. This study utilized Ribo seq technology to construct a transcriptome map of yak testicular development, revealing that the expression of genes related to spermatogenesis is specifically translated and regulated at different developmental stages. more...

Organism:

Bos grunniens; Bos grunniens x Bos taurus

Type:

Other

Platforms:

GPL34611 GPL30466

12 Samples

Download data: TXT

(Submitter supplied) The testis is an important reproductive organ in male mammals, serving as the site for spermatogenesis, androgen synthesis, and secretion. Non-coding RNAs (ncRNAs) plays an important regulatory role in various biological processes. However, the regulatory role of ncRNAs on yak testicular development and spermatogenesis is still largely unclear. In this study, the expression profiles of circRNA, miRNA, and mRNA in testicular tissues were compared between 6 months (Y6M), 18 months (Y18M), and 4 years old (Y4Y), with a significant difference in testicular development using RNA-seq. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL30409

18 Samples

Download data: TXT

(Submitter supplied) The epididymis is an important component of the male reproductive system and a crucial site for sperm maturation. During the process of sperm maturation through the epididymis, the effective payload of SncRNA significantly changes, which may be related to epigenetic modifications. Unfortunately, the N6 Methyladenosine (m6A) modification profile of the epididymis has not yet been established. In this study, the MeRIP seq combined with RNA seq method was used to analyze the m6A modification levels in the head, body, and tail of yak epididymis. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL30409

18 Samples

Download data: TXT

(Submitter supplied) This study explores how yaks, an ideal animal model for studying plateau adaptability, adapt to high-altitude environments. The lung is a representative organ of the yak’s adaptation to high-altitude environments. The F1 hybrids of yak and cattle, known as dzho, also exhibit adaptability to plateau conditions. This study constructed a single-cell transcriptome atlas of the lungs in yak, dzho and cattle, containing 51 subtypes. more...

Organism:

Bos grunniens; Bos taurus; Bos grunniens x Bos taurus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32644 GPL26012 GPL30409

9 Samples

Download data: MTX, TSV

(Submitter supplied) Alternative splicing (AS) is strictly regulated during cell differentiation and development. AS events are common in the testis, but the splicing regulation in spermatogenesis is still unclear. In this experiment, the third-generation ONT sequencing was used to sequence the full-length transcriptome of testicular tissue, and 40,038 new transcripts were obtained, and the proportion was almost close to the number of known transcripts identified. more...

Organism:

Bos grunniens x Bos taurus; Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL34048 GPL34047

15 Samples

Download data: FA, TXT

(Submitter supplied) Cattle-yak is the hybrid offspring of yak and cattle. It has obvious heterosis in production performance, but the male sterility of cattle-yak has always been the focus of attention. Studies have shown that non-coding RNA is involved in the regulation of spermatogenesis. We comprehensively compared the testicular transcription profiles of cattle, yak and cattle-yak. More DEGs, DECs and DEMs were found in the intersection of the two comparison groups of cattle and cattle-yak, yak and cattle-yak, with 4,968, 360 and 59, respectively. more...

Organism:

Bos taurus; Bos grunniens x Bos taurus; Bos grunniens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL19172 GPL32499 GPL22950

18 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL26759 GPL22950

12 Samples

Download data

(Submitter supplied) Dermal papilla cells (DPCs) and epidermal hair matrix cells (HMCs) located in hair bulb are the key cell types during the hair follicles (HFs) development. To explore the mRNA and miRNA expression of DPCs and HMCs of yak hair follicle, illustrating the mocular basis of the interaction and cellular communication between DPCs and HMCs during the hair follicle development, the DPCs and HMCs of yak were isolated and cultured, RNA-seq was used to identify the differentially expressed mRNAs and miRNAs between DPCs and HMCs.

Organism:

Bos grunniens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22950

6 Samples

Download data: TXT

(Submitter supplied) Dermal papilla cells (DPCs) and epidermal hair matrix cells (HMCs) located in hair bulb are the key cell types during the hair follicles (HFs) development. To explore the mRNA and miRNA expression of DPCs and HMCs of yak hair follicle, illustrating the mocular basis of the interaction and cellular communication between DPCs and HMCs during the hair follicle development, the DPCs and HMCs of yak were isolated and cultured, RNA-seq was used to identify the differentially expressed mRNAs and miRNAs between DPCs and HMCs.

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26759

6 Samples

Download data: TXT

(Submitter supplied) Testis is the most important male reproductive organ, and the integrity of its physiological function is crucial to the successful production of sperm. In this study, the expression profiles of 11 991 and 8 930 cells in testicular tissue of yak and cattle-yak after sexual maturity were established using Single-cell RNA sequencing. The identification results of cell subpopulations and marker genes were analyzed and their possible mechanisms were predicted.

Organism:

Bos grunniens; Bos grunniens x Bos taurus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32644 GPL30409

2 Samples

Download data: MTX, TSV

(Submitter supplied) This study provides a clear and accurate dynamic transcriptome profile of mRNAs in rumen, reticulum, omasum and abomasum of yaks. The results include high-quality genomic data and help to elucidate the important roles of these mRNAs in regulation of growth, development and metabolism in yaks, and to further understand the molecular mechanisms underlying metabolic regulation of yak stomach tissues. At the same time, it provided a theoretical basis for age-appropriate weaning and supplementary feeding in yaks.

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22950

60 Samples

Download data: XLS

(Submitter supplied) Studying spermatogenesis is the key to understanding the development mechanism of the yak reproductive system. Although N6-methyladenosine (m6A) RNA modification has been reported to regulate spermatogenesis and reproductive function in mammals, the molecular mechanism of m6A in yak testis development and spermatogenesis remains largely unknown. Therefore, we collected testicular tissue from junile and adult yaks, and found that the m6A level significantly increased after sexual maturity in yak. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:

GPL30409

6 Samples

Download data: TXT

(Submitter supplied) A cellular atlas of yak ovary was established successfully, containing oocytes, granulosa cells, stromal cells, endothelial cells, smooth muscle cells, natural killer cells, and macrophages. A cell-to-cell communication network was constructed in yak ovary.

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30409

1 Sample

Download data: MTX, TSV

(Submitter supplied) Hair follicles of the yak are in anagen and catagen , the hair follicles of healthy female yaks around 2 years old are collected for the preparation of single-cell suspension, the cells were sequenced by scRNA-seq on the 10x genome platform. A total of about 12000 single-cell transcriptome information were obtained. According to the reported marker genes, the main cell groups in anagen and catagen of yak were identified. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30409

3 Samples

Download data: MTX, TSV

(Submitter supplied) The Jeryak is the hybrid offspring of yaks and Jersey cattle and exhibit improved milk and meat yields. Biomolecules carried within milk exosomes are important for cell growth, development, immune regulation, and various pathophysiological processes. Previous studies showed that miRNAs regulate mammary gland development, lactation, and milk quality. This study explored the relationship between milk exosomes miRNAs and lactation performance. more...

Organism:

Bos grunniens x Bos taurus; Bos grunniens

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL32644 GPL30409

12 Samples

Download data: XLSX

(Submitter supplied) The scRNA-seq technology was performed to delineate an atlas of molecular signature of various cell types in yak ovary. The cell types were identified according to their specifically expressed genes and the functional enrichment of the genes.

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30409

1 Sample

Download data: MTX, TSV

(Submitter supplied) RNA sequence analysis identified a total of 8473 differentially expressed genes (DEGs) (3580 up-regulated, 4893 down-regulated), and GO annotation and KEGG enrichment of differential genes found that DEGS were mostly related to spermatogenesis and apoptosis. Among them, Spermatogonia stem cell (SSCs) marker genes (ITGA5, Gfra1, CD9, SOHLH1, SALL4, ID4 and FOXO1) were significantly up-regulated, differentiation spermatogenic cell marker genes (KIT, UCHL1, Ccna1, TNP1 and TXNDC2) were significantly down-regulated, and genes involved in apoptosis (Fas, CTSK, CTSB, CTSC, CTSO, caspase 3, caspase6, and caspase) were significantly up-regulated. more...

Organism:

Bos grunniens x Bos taurus; Bos grunniens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32499 GPL22950

6 Samples

Download data: TXT

(Submitter supplied) N6-methyladenosine (m6A) is the most prominent mRNA modification in eukaryotes, and its potential regulatory role has recently been identified in mammals, plants, and yeast. However, how m6A methylation regulates spermatogenesis remains unknown. In this study, cattle-yak testis tissue was used as the experimental material, and the m6A map was generated through preliminary experiments and methylated RNA immunoprecipitation sequencing. more...

Organism:

Bos grunniens

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL30466

6 Samples

Download data: TXT

(Submitter supplied) Dynamic transcriptome profiles in yak mammary gland were evaluated by sampling mammary tissue at -30, -15, 1, 15, 30, 60, 120, 180 and 240 d relative to parturition and using a bovine commercial microarray platform. Several bioinformatics tools were used to analyze the data, both as expression relative to -30 d and as expression relative to the previous time point. There were >6,000 differentially expressed transcript (DEG; FDR＜0.05; P＜0.05) throughout lactation with larger DEG observed at the onset (1 vs. more...

Organism:

Bos taurus; Bos grunniens

Type:

Expression profiling by array

Platform:

GPL11649

36 Samples

Download data: TXT

(Submitter supplied) N6-methyladenosine (m6A) is the most common internal modification of eukaryotic mRNA, and its potential regulatory role has recently been identified in mammals, plants, and yeast. However, little is known about the role of m6A methylation in mammalian sexual maturation. In this study, the testicular tissue of yaks before and after sexual maturation was taken as the research object, and the m6A map of testicular tissue of yaks before and after sexual maturation was obtained through the preliminary experiment and MERIP-seq. more...

Organism:

Bos grunniens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL30466

12 Samples

Download data: TXT