GEO DataSets

(Submitter supplied) The immunomodulatory mycobacterial surface antigen lipoarabinomannan (LAM) with its immunogenic glycan component arabinomannan (AM) facilitates Mycobacterium tuberculosis’ (Mtb) escape from the host’s immune response. Some but not all antibodies against AM can protect against TB. To better understand which of AM’s structures to target, we must first identify the spectrum of its epitopes. Through isolating and characterizing a panel of novel human mAbs with binding to distinct AM OS motifs, we further defined the glycan epitope structures, identified a new epitope, and determined their differences among mycobacterial strains.

Organism:

synthetic construct; Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL34369

11 Samples

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(Submitter supplied) Microarray slides were printed at Engineering Arts LLC (Phoenix, AZ). BSA conjugates were noncontact printed on Schott type E slides (Schott North America, Inc., Elmsford, NY) using an Engineering Arts au301 Rainmaker microarray printer and dispensed at 100 μg/mL in buffer (1:10 phosphate-buffered saline plus 0.005% vol/vol Triton X-100). Antigens were dispensed at 360 pL per spot to produce 140- to 160-m diameter spots in triplicate. more...

Organism:

synthetic construct

2 Series

118 Samples

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