GSM806979[Accession] - GEO DataSets

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Items: 3

Select item 2000325511.

Stage-specific transcriptional changes during zygotic embryogenesis in maritime pine (Pinus pinaster Ait.)

(Submitter supplied) The significant morphological differences observed during embryo development in angiosperms and gymnosperms are expected to be the result of a differential control of gene expression. We used a loblolly pine (Pinus taeda) cDNA microarray to analyze global transcriptional changes along zygotic embryogenesis in maritime pine (Pinus pinaster). A time-course analysis of the data obtained from the five embryo developmental groups used in this study led to the identification of 4,645 genes whose expression varied along P. more...

Organism:: Pinus pinaster; Pinus taeda

Type:: Expression profiling by array

Platform:: GPL11184
30 Samples

Download data: TXT

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Accession:: GSE32551

ID:: 200032551

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000111842.

Loblolly pine 26K cDNA array v2.0, PtGen2

(Submitter supplied) Plasmid DNAs that had been propagated in the EST sequencing pipeline were re-arrayed and consolidated into new plates to using a BioMek 2000 liquid handling robot (Beckman Coulter, Fullerton, CA). Samples were then diluted five-fold in millipure dH20 (mp dH20) and 5mL was transferred to a 96-well plate (ABgene AB-0900, Thermo Fisher Scientific Inc. Waltham, MA) for PCR amplification. PCR reactions were performed in a 100mL volume containing 10 mM Tris (pH 8.8), 3.5 mM MgCl2, 25 mM KCl, 0.25 mM (ea.) dNTP, 0.4 mM pSLFOR2 primer {5’-AAAACGACGGCCAGTGCCAAG-3’}, 0.4mM pSLREV2 primer {5’-GCTTCCGGCTGCTATGTTGTGTGG-3’}, and 1 mL of homemade Taq polymerase. more...