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Items: 3

1.

Scaffold RNA engineering in type V CRISPR-Cas systems: a potent way to enhance gene expression in the yeast Saccharomyces cerevisiae.

(Submitter supplied) New, orthogonal transcription factors in eukaryotic cells have been realized by engineering nuclease-deficient CRISPR-associated proteins and/or their guide RNAs. In this work, we present a new kind of orthogonal activators, in Saccharomyces cerevisiae, made by turning type V CRISPR RNA into a scaffold RNA (ScRNA) able to recruit a variable number of VP64 activation domains. The activator arises from the complex between the synthetic ScRNA and DNase-deficient type V Cas proteins: dCas12e and denAsCas12a. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33987
4 Samples
Download data: XLS
Series
Accession:
GSE249355
ID:
200249355
2.

DNBSEQ-T7 (Saccharomyces cerevisiae)

Organism:
Saccharomyces cerevisiae
3 Series
16 Samples
Download data
Platform
Accession:
GPL33987
ID:
100033987
3.

byMM1736

Organism:
Saccharomyces cerevisiae
Source name:
Control strain, wihtout ScRNA expression cassette, for dCas12e:ScRNA activator
Platform:
GPL33987
Series:
GSE249355
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Sample
Accession:
GSM7937423
ID:
307937423
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Supplemental Content

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