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1.

Age-associated chromatin re-organization in progenitor B cells

(Submitter supplied) Three-dimensional (3D) organization of the genome is essential for precise patterns of gene expression required for biological processes, however its role in physiological aging is not known.Here we show that large scale chromatin re-organization distinguishes bone marrow progenitor (pro-) B cells of old mice from that of young mice.These changes result in increased interactions at the compartment level and reduced interactions within topologically associated domains (TADs).One genomic region that transitions from compartment A to B with age contains the gene encoding Ebf1, a key regulator of normal B cell development.Genetically reducing Ebf1 recapitulates some features of old pro-B cells.TADs that are most reduced with age also harbor genes important for function and development of pro-B cells, including the immunoglobulin heavy chain (IgH) gene locus.Weaker intra-TAD interactions at IgH correlate with reduced utilization of distally located variable gene segments in VDJ recombination.Our observations implicate 3D chromatin re-organization as a major driver of pro-B cell phenotypes that impair B lymphopoiesis with age.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other; Genome variation profiling by high throughput sequencing
Platforms:
GPL16417 GPL24247 GPL19057
76 Samples
Download data: BED, BEDPE, TXT
Series
Accession:
GSE214438
ID:
200214438
2.

Illumina NovaSeq 6000 (Mus musculus)

Platform
Accession:
GPL24247
ID:
100024247
3.

ChIP-seq_Young_Brg1_rep2

Organism:
Mus musculus
Source name:
progenitor B cells
Platform:
GPL24247
Series:
GSE214438
Download data: BED, BEDPE
Sample
Accession:
GSM7921749
ID:
307921749
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db=gds|term=GSM7921749[Accession]|query=1|qty=2|blobid=MCID_66983042deb7cc0864a19379|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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