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Items: 3

1.

tRNA structure-seq in peptide-rich droplets

(Submitter supplied) Compartmentalization of RNA in biopolymer-rich membraneless organelles is now understood to be pervasive and critical for the function of extant biology, and has been proposed as a prebiotically-plausible way to accumulate RNA. Compartment-RNA interactions that drive encapsulation have the potential to influence RNA structure and function in compartment- and RNA sequence-dependent ways. Herein, we detail Next-Generation Sequencing (NGS) experiments performed for the first time in membraneless compartments called complex coacervates to characterize the fold of many different transfer RNAs (tRNAs) simultaneously under the potentially denaturing conditions of these compartments. more...
Organism:
synthetic construct; Escherichia coli
Type:
Other
Platforms:
GPL32081 GPL32628
60 Samples
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Series
Accession:
GSE225730
ID:
200225730
2.

NextSeq 2000 (Escherichia coli)

Organism:
Escherichia coli
13 Series
539 Samples
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Platform
Accession:
GPL32081
ID:
100032081
3.

NM E. coli tRNAs DMS treated 10mers / 1:2 / 10 replicate 3

Organism:
Escherichia coli
Source name:
E. coli
Platform:
GPL32081
Series:
GSE225730
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Sample
Accession:
GSM7054843
ID:
307054843
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