GSM6422268[Accession] - GEO DataSets

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Items: 4

Select item 2002253141.

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Other

Platforms:: GPL29177 GPL28352
28 Samples

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Series

Accession:: GSE225314

ID:: 200225314

PubMed Full text in PMC Similar studies

Select item 2002101362.

Direct transposition of native DNA for sensitive multimodal single-molecule sequencing I

(Submitter supplied) Concurrent readout of sequence and base modifications from long unamplified DNA templates by PacBio single-molecule sequencing requires large amounts of input material. Here we adapt Tn5 transposition to introduce hairpin oligonucleotides and fragment (tagment) limiting quantities of DNA for generating PacBio-compatible circular molecules. We developed two methods that implement tagmentation and use 90–99% less input than current protocols: (1) single-molecule real-time sequencing by tagmentation (SMRT-Tag), which allows detection of genetic variation and CpG methylation; and (2) single-molecule adenine-methylated oligonucleosome sequencing assay by tagmentation (SAMOSA-Tag), which uses exogenous adenine methylation to add a third channel for probing chromatin accessibility. more...