GSM619375[Accession] - GEO DataSets

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Items: 3

Select item 2000252161.

Microarray analysis and scale-free gene networks identify candidate regulators in drought-stressed roots of loblolly pine (P. taeda L.).

(Submitter supplied) Global transcriptional analysis of loblolly pine (Pinus taeda L.) is challenging due to limited molecular tools. PtGen2, a 26,496 feature cDNA microarray, was fabricated and used to assess drought-induced gene expression in loblolly pine propagule roots. Statistical analysis of differential expression and weighted gene correlation network analysis were used to identify drought-responsive genes and further characterize the molecular basis of drought tolerance in loblolly pine.

Organism:: Pinus taeda

Type:: Expression profiling by array

Platform:: GPL11184
144 Samples

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Accession:: GSE25216

ID:: 200025216

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000111842.

Loblolly pine 26K cDNA array v2.0, PtGen2

(Submitter supplied) Plasmid DNAs that had been propagated in the EST sequencing pipeline were re-arrayed and consolidated into new plates to using a BioMek 2000 liquid handling robot (Beckman Coulter, Fullerton, CA). Samples were then diluted five-fold in millipure dH20 (mp dH20) and 5mL was transferred to a 96-well plate (ABgene AB-0900, Thermo Fisher Scientific Inc. Waltham, MA) for PCR amplification. PCR reactions were performed in a 100mL volume containing 10 mM Tris (pH 8.8), 3.5 mM MgCl2, 25 mM KCl, 0.25 mM (ea.) dNTP, 0.4 mM pSLFOR2 primer {5’-AAAACGACGGCCAGTGCCAAG-3’}, 0.4mM pSLREV2 primer {5’-GCTTCCGGCTGCTATGTTGTGTGG-3’}, and 1 mL of homemade Taq polymerase. more...