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Items: 3

1.

An extension to: Systematic assessment of commercially available low-input miRNA library preparation kits

(Submitter supplied) We compared the performance of two commercial kits (NEBNext and NEXTflex) for miRNA detection sensitivity, reliability, titration response and ability to detect differentially expressed miRNAs at different amounts of synthetic miRNAs. We observed differences in detection sensitivity between the kits, but none were able to detect the full repertoire of expected miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the majority of miRNAs. more...
Organism:
Homo sapiens; synthetic construct
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL19604 GPL16791
42 Samples
Download data: CSV, FASTA
Series
Accession:
GSE141658
ID:
200141658
2.

Illumina HiSeq 2500 (synthetic construct)

Organism:
synthetic construct
57 Series
5350 Samples
Download data
Platform
Accession:
GPL19604
ID:
100019604
3.

NEXTflex C 2

Organism:
synthetic construct
Source name:
Synthetic miRNA
Platform:
GPL19604
Series:
GSE141658
Download data
Sample
Accession:
GSM4210691
ID:
304210691
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