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Items: 4

1.

Arid1a in mouse embryonic cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL21273
149 Samples
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Series
Accession:
GSE140925
ID:
200140925
2.

Identification of the dedifferentiation trajectory of the cells without Arid1a using single cell RNA-seq

(Submitter supplied) The primary mouse hepatocytes were isolated from the liver tissue Arid1af/f mouse. To immortalize mouse primary hepatocytes, the isolated cells were first infected with lentivirus expressing simian virus 40 large-T protein (SV40LT), and then cultured until proliferated cell colonies appear. Cells were further transformed with lentivirus expressing HrasV12D to generate a cell line called AB17, which were cultured in DMEM supplemented with 10% FBS and 100 µg/ml penicillin-streptomycin in a humidified incubator at 37℃ with 5% CO2.To get insight into trajectory of dedifferentiation induced by Arid1a loss, we performed single-cell RNA sequencing (scRNA-seq) on FACS-sorted GFP+ AB17 cells using Fluidigm C1 platform, and obtained the scRNA-seq data from 76 cells with Arid1a knockout induced by Ad-Cre and 73 cells infected with Ad-GFP as control. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21273
149 Samples
Download data: TXT
Series
Accession:
GSE140924
ID:
200140924
3.

HiSeq X Ten (Mus musculus)

Platform
Accession:
GPL21273
ID:
100021273
4.

WL2C5

Organism:
Mus musculus
Source name:
AB17
Platform:
GPL21273
Series:
GSE140924 GSE140925
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Sample
Accession:
GSM4190459
ID:
304190459
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