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Items: 3

1.

Single cell chromatin immunocleavage sequencing (scChIC-Seq) to profile histone modification

(Submitter supplied) We report a single-cell chromatin immunocleavage sequencing (scChIC-seq) methodology for analyzing histone modifications, which involves targeting of the micrococcal nuclease (MNase) by tethering it to an antibody and selective PCR amplification of cleaved target sites. We show that the protocol reliably detects the H3K4me3 and H3K27me3 target sites in single human white blood cells (WBC), resulting data for successful identification of unique blood cell types based on clustering analysis. more...
Organism:
Homo sapiens; Mus musculus
Type:
Genome binding/occupancy profiling by high throughput sequencing; Other
Platforms:
GPL13112 GPL21290 GPL21493
425 Samples
Download data: BED, BEDGRAPH, TXT
Series
Accession:
GSE105012
ID:
200105012
2.

Illumina HiSeq 3000 (Mus musculus)

Platform
Accession:
GPL21493
ID:
100021493
3.

3T3 fixed frozen 3000 cells IgG Ab + PA-MNase, 400 mM salt wash buffer

Organism:
Mus musculus
Source name:
NIH/3T3 tet-on 3G cells
Platform:
GPL21493
Series:
GSE105012
Download data: BED, BEDGRAPH
Sample
Accession:
GSM3475874
ID:
303475874
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Supplemental Content

db=gds|term=GSM3475874[Accession]|query=1|qty=3|blobid=MCID_66221899e288b445756e9f57|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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