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Items: 3

1.

En bloc and segmental deletions of human XIST reveal X chromosome inactivation-involving RNA elements

(Submitter supplied) RNA-seqs followed by whole and segmental deletions of XIST genes in human K562 cells. The XIST RNA is a non-coding RNA that induces X chromosome inactivation (XCI). Unlike the mouse Xist RNA, how the human XIST RNA controls XCI in female cells is less well characterized, and the XCI-involving RNA elements remain unclear. To systematically decipher the XCI-involving elements of XIST RNA, ten smaller XIST segments, including repeats A, D, and E; human-specific repeat elements; the promoter; and non-repetitive exons, as well as the entire XIST gene, were homozygously deleted using the Cas9 nuclease and paired guide RNAs at high efficiencies, followed by high-throughput RNA sequencing and fluorescence in-situ hybridization experiments on XIST RNA.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Other
Platforms:
GPL11154 GPL16791
29 Samples
Download data: TXT, VCF
2.

Illumina HiSeq 2500 (Homo sapiens)

Platform
Accession:
GPL16791
ID:
100016791
3.

SD1-14

Organism:
Homo sapiens
Source name:
clone containing segmental deletion of 3' splice site of exon 1
Platform:
GPL16791
Series:
GSE65830
Download data: TXT
Sample
Accession:
GSM3381512
ID:
303381512
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