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Items: 3

1.

Distinct 5-methylcytosine profiles in poly(A)RNA from mouse bone marrow derived mast cells

(Submitter supplied) Purpose: To transcriptome widely reveal 5-mC regulation by Tet2, we performed bisulfite-sequencing of mRNAs from Tet2-deficient BMMCs and the control cells.Methods: Mouse BMMCs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse IL-3 and SCF. BMMCs were stained to confirm the surface expression of FcɛRI and c-Kit. Cells with purity >97.5% were used for RNA isolation. Results: We carried out an unbiased global analysis of m5C in poly(A)RNA of mouse bone marrow derived mast cells (BMMCs).We show that there were indeed much more mCs in Tet2-deficient group compared with the control, more than half of which located in genic region. more...
Organism:
Mus musculus
Type:
Methylation profiling by high throughput sequencing
Platform:
GPL21273
8 Samples
Download data: TXT
Series
Accession:
GSE100719
ID:
200100719
2.

HiSeq X Ten (Mus musculus)

Platform
Accession:
GPL21273
ID:
100021273
3.

WT-biological replicate 2-technical replicate 2

Organism:
Mus musculus
Source name:
bone marrow derived mast cell
Platform:
GPL21273
Series:
GSE100719
Download data
Sample
Accession:
GSM2692019
ID:
302692019
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