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Items: 4

1.

Systematic assessment of next-generation sequencing for quantitative small RNA profiling: a multiple protocol study across multiple laboratories

(Submitter supplied) Small RNA-seq is increasingly being used for profiling of small RNAs. Quantitative characteristics of long RNA-seq have been extensively described, but small RNA-seq involves fundamentally different methods for library preparation, with distinct protocols and technical variations that have not been fully and systematically studied. We report here the results of a study using common references (synthetic RNA pools of defined composition, as well as plasma-derived RNA) to evaluate the accuracy, reproducibility and bias of small RNA-seq library preparation for five distinct protocols and across nine different laboratories. more...
Organism:
synthetic construct; Homo sapiens
Type:
Non-coding RNA profiling by high throughput sequencing
7 related Platforms
149 Samples
Download data: TXT
Series
Accession:
GSE94586
ID:
200094586
2.

Systematic assessment of next-generation sequencing for quantitative small RNA profiling: synthetic ratiometric pools

(Submitter supplied) Small RNA-seq is increasingly being used for profiling of small RNAs. Quantitative characteristics of long RNA-seq have been extensively described, but small RNA-seq involves fundamentally different methods for library preparation, with distinct protocols and technical variations that have not been fully and systematically studied. Using common sets of reference samples, we evaluated the accuracy, reproducibility and bias of small RNA-seq library preparation for five distinct protocols and across nine different laboratories. more...
Organism:
synthetic construct
Type:
Non-coding RNA profiling by high throughput sequencing
4 related Platforms
50 Samples
Download data: TXT
Series
Accession:
GSE94585
ID:
200094585
3.

Illumina HiSeq 2500 (synthetic construct)

Organism:
synthetic construct
54 Series
5348 Samples
Download data
Platform
Accession:
GPL19604
ID:
100019604
4.

4N_Xu.Lab5.SynthB

Organism:
synthetic construct
Source name:
Synthetic smallRNA; Ratiometric Pool B
Platform:
GPL19604
Series:
GSE94585 GSE94586
Download data: TXT
Sample
Accession:
GSM2478914
ID:
302478914
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