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Items: 3

1.

Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome

(Submitter supplied) mRNAs of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~100 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2000/3000 (Illumina) in pair-end mode, creating reads with a length of 101 bp. more...
Organism:
Mus musculus; Homo sapiens
Type:
Other
4 related Platforms
31 Samples
Download data: TXT
Series
Accession:
GSE74771
ID:
200074771
2.

Illumina HiSeq 3000 (Homo sapiens)

Platform
Accession:
GPL21290
ID:
100021290
3.

ALYREF-IP_rep2BS

Organism:
Homo sapiens
Source name:
ALYREF-IP human HeLa cells - bisulfite-treated mRNAs
Platform:
GPL21290
Series:
GSE74771
Download data: TXT
Sample
Accession:
GSM2176248
ID:
302176248
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