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Items: 4

1.

The effect of the Pld6 and Dnmt3l mutations on DNA methylation and expression of retrotransposons in mouse male germ cells

(Submitter supplied) Mammalian genomes harbor millions of retrotransposon copies, some of which are transpositionally active. In mouse prospermatogonia, PIWI-interacting small RNAs called piRNAs combat retrotransposon activity. The piRNA system guides de novo DNA methylation at retrotransposon promoters, but it remains unclear whether DNA methylation is involved in retrotransposon silencing in prospermatogonia. We performed a genome-wide study of DNA methylation and RNA abundance for retrotransposons in developing mouse male germ cells, using Pld6/Mitopld and Dnmt3l knockout (KO) mice deficient in piRNA biogenesis and de novo DNA methylation, respectively. more...
Organism:
Mus musculus
Type:
Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL18480 GPL17021
37 Samples
Download data: CSV, TXT
Series
Accession:
GSE70891
ID:
200070891
2.

Small RNA sequencing analysis for male germ cells during spermatogenesis

(Submitter supplied) Small RNAs in FACS-purified spermatogonia, spermatocytes (L/Z and P/D) and round spermatids were sequenced to study piRNA populations.
Organism:
Mus musculus
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL18480 GPL17021
13 Samples
Download data: CSV, TXT
Series
Accession:
GSE70890
ID:
200070890
3.

Illumina HiSeq 2500 (Mus musculus)

Platform
Accession:
GPL17021
ID:
100017021
4.

small_RNA_seq: leptotene/zygotene, biological replicate 2, technical replicate 1

Organism:
Mus musculus
Source name:
male germ cells
Platform:
GPL17021
Series:
GSE70890 GSE70891
Download data: CSV, TXT
Sample
Accession:
GSM2080811
ID:
302080811
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