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Items: 3

1.

Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant

(Submitter supplied) We expressed either a wt or a phosphomutant version of ZFP36L1 in IMR90 ER:RAS cells. 7 days upon RAS induction (when the cells reach a fully senescent phenotype) we collected the RNA. ZFP36L1 is a RNA binding protein that binds to AU-rich elements in the 3’UTR of mRNAs and triggers their degradation. Our previous experiments showed that the activity of ZFP36L1 was key in the regulation of the senescent phenotype.By performing RNAseq we have uncovered the effect of expressing a constitutively active mutant of ZFP36L1 within the senescent transcriptome.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
12 Samples
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2.

Illumina HiSeq 2500 (Homo sapiens)

Platform
Accession:
GPL16791
ID:
100016791
3.

ZFP36L1mut +4OHT Rep3

Organism:
Homo sapiens
Source name:
IRM90 ER:RAS
Platform:
GPL16791
Series:
GSE61130
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Sample
Accession:
GSM1498130
ID:
301498130
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