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Items: 3

1.

High-resolution mapping of chromatin packaging in mouse embryonic stem cells and sperm

(Submitter supplied) Mammalian embryonic stem (ES) cells and sperm exhibit unusual chromatin packaging that plays important roles in cellular function. Here, we extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine ES cells and sperm. In ES cells, we recover well-characterized features of chromatin such as promoter nucleosome depletion, and further identify widespread footprints of sequence-specific DNA-binding proteins such as CTCF, which we validate in knockdown studies. more...
Organism:
Mus musculus
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL16417 GPL13112
17 Samples
Download data: BW, TXT
Series
Accession:
GSE58101
ID:
200058101
2.

Illumina MiSeq (Mus musculus)

Platform
Accession:
GPL16417
ID:
100016417
3.

MNase Sperm Pellet 5 Units

Organism:
Mus musculus
Source name:
Sperm, 5 units MNase, pellet
Platform:
GPL16417
Series:
GSE58101
Download data: TXT
Sample
Accession:
GSM1400780
ID:
301400780
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