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Items: 3

1.

ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs

(Submitter supplied) We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage.
Organism:
Mus musculus
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL9250 GPL13112
30 Samples
Download data: WIG
Series
Accession:
GSE35496
ID:
200035496
2.

Illumina HiSeq 2000 (Mus musculus)

Platform
Accession:
GPL13112
ID:
100013112
3.

Brn2 ChIP-Seq Day2 rtta, rep1

Organism:
Mus musculus
Source name:
Day2 differentiating rtta ESCs, Brn2 ChIP
Platform:
GPL13112
Series:
GSE35496
Download data
Sample
Accession:
GSM1050297
ID:
301050297
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